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Home › Products › Gene function › Fluorescent proteins › Fluorescent protein plasmids › Red fluorescent proteins › DsRed2 fluorescent protein

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DsRed2 fluorescent protein

Vectors for expression of the DsRed2 fluorescent protein

DsRed2 retains the benefits typical of red fluorescent proteins, such as a high signal-to-noise ratio and distinct spectral properties for use in multicolor labeling experiments. DsRed2 is a variant of Discosoma sp. red fluorescent protein (drFP583) (Matz et al. 1999) with faster maturation and lower non-specific aggregation.

DsRed2 retains the benefits typical of red fluorescent proteins, such as a high signal-to-noise ratio and distinct spectral properties for use in multicolor labeling experiments. DsRed2 is a variant of Discosoma sp. red fluorescent protein (drFP583) (Matz et al. 1999) with faster maturation and lower non-specific aggregation. The DsRed2 sequence contains a series of silent base pair changes corresponding to human codon usage preferences for high expression in mammalian cells (Haas, Park, and Seed 1996). You may also be interested in DsRed-Express2, an alternative DsRed version with mutations that enhance its solubility, reduce its green emission, and accelerate its maturation.

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Cat. # Product Size Price License Quantity Details
632404 pDsRed2 Vector 20 ug USD $615.00

pDsRed2 encodes DsRed2, a variant of the Discosoma sp. red fluorescent protein (DsRed). DsRed2 contains six amino acid substitutions that accelerate chromophore maturation and decrease protein aggregation. In addition, the coding sequence for DsRed2 contains a series of silent base-pair changes for higher expression in mammalian cells. In pDsRed2, the DsRed2 coding sequence is flanked by two separate MCS regions at the 5' and 3' ends, making it possible to excise and shuttle the DsRed2 coding region into other expression vectors. pDsRed2 is intended to serve as a source of the DsRed2 cDNA; It can also be used in bacteria to produce DsRed2 protein.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1

Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1

Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2,  and HcRed1.

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Basic fluorescent protein vector map

Basic fluorescent protein vector map

Basic fluorescent protein vector map. Use this bacterial expression vector as a source of the fluorescent protein gene. Note: There is a stop codon at the 5' end of the 3' MCS. The 3' MCS should not be used for cloning.

632405 pDsRed2-1 Vector 20 ug USD $615.00

pDsRed2-1 encodes DsRed2, a variant of the Discosoma sp. red fluorescent protein (DsRed). DsRed2 contains six amino acid substitutions that accelerate chromophore maturation and decrease protein aggregation. In addition, the coding sequence for DsRed2 contains a series of silent base-pair changes for higher expression in mammalian cells. pDsRed2-1 can be used as a reporter to monitor the activity of promoters and promoter/enhancer combinations cloned into the multiple cloning site (MCS) upstream of the promoterless DsRed2 gene. Without the addition of a functional promoter, this vector will not express DsRed2.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Vector map for promoterless fluorescent protein vectors AcGFP1-1, DsRed2-1, DsRed-Express-1, HcRed1-1 and ZsGreen1-1

Vector map for promoterless fluorescent protein vectors AcGFP1-1, DsRed2-1, DsRed-Express-1, HcRed1-1 and ZsGreen1-1

Vector map for promoterless fluorescent protein vectors pAcGFP1-1, pDsRed2-1, pDsRed-Express-1, pHcRed1-1 and pZsGreen1-1.

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Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1

Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1

Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2,  and HcRed1.

632406 pDsRed2-N1 Vector 20 ug USD $615.00

pDsRed2-N1 encodes DsRed2, a variant of Discosoma sp. red fluorescent protein, (DsRed). DsRed2 contains six amino acid substitutions that accelerate chromophore maturation and decrease protein aggregation. In addition, the coding sequence for DsRed2 is human codon-optimized for higher expression in mammalian cells. pDsRed2-N1 allows genes to be cloned into the multiple cloning site (MCS) upstream of the DsRed2 coding sequence to be expressed as fusions to the N-terminus of DsRed2. The unmodified vector will express DsRed2 in mammalian cells.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1

Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2, and HcRed1

Excitation and emission spectra of reef coral fluorescent proteins AmCyan1, ZsGreen1, ZsYellow1, DsRed2, AsRed2,  and HcRed1.

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Schematic for fluorescent fusion protein vectors

Schematic for fluorescent fusion protein vectors

Schematic for fluorescent fusion protein vectors. Panel A. N-terminal fluorescent protein (FP) vector. Panel B. C-terminal fluorescent protein vector.

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Hygromycin DsRed-Monomer and AcGFP1 expression vectors

Hygromycin DsRed-Monomer and AcGFP1 expression vectors

Hygromycin DsRed-Monomer and AcGFP1 expression vectors. Vector maps of our DsRed-Monomer and pAcGFP1 Hyg-N1 (Panel A) and Hyg-C1 (Panel B) mammalian expression vectors for fusion applications. Each vector contains a hygromycin selection cassette. These vectors can be used together with our standard N1/C1 vectors containing the neomycin selection cassette to establish double stable cell lines expressing two different Living Colors proteins.

632407 pDsRed2-C1 Vector 20 ug USD $615.00

pDsRed2-C1 encodes DsRed2, a variant of the Discosoma sp. red fluorescent protein (DsRed) that has been engineered for faster maturation and lower, non-specific aggregation. DsRed2 contains six amino acid substitutions that accelerate chromophore formation and decrease protein aggregation. In addition, the coding sequence for DsRed2 is human codon-optimized for high expression in mammalian cells. pDsRed2-C1 allows genes cloned into the multiple cloning site (MCS) downstream of the DsRed2 coding sequence to be expressed as fusions to the C-terminus of DsRed2 in mammalian cells.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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632407: pDsRed2-C1 Vector

632407: pDsRed2-C1 Vector

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Schematic for fluorescent fusion protein vectors

Schematic for fluorescent fusion protein vectors

Schematic for fluorescent fusion protein vectors. Panel A. N-terminal fluorescent protein (FP) vector. Panel B. C-terminal fluorescent protein vector.

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Hygromycin DsRed-Monomer and AcGFP1 expression vectors

Hygromycin DsRed-Monomer and AcGFP1 expression vectors

Hygromycin DsRed-Monomer and AcGFP1 expression vectors. Vector maps of our DsRed-Monomer and pAcGFP1 Hyg-N1 (Panel A) and Hyg-C1 (Panel B) mammalian expression vectors for fusion applications. Each vector contains a hygromycin selection cassette. These vectors can be used together with our standard N1/C1 vectors containing the neomycin selection cassette to establish double stable cell lines expressing two different Living Colors proteins.

632408 pDsRed2-Nuc Vector 20 ug USD $615.00

pDsRed2-Nuc encodes Discosoma sp. red fluorescent protein (DsRed2) fused with three copies of the nuclear localization signal (NLS) of the SV40 T-antigen. The NLS sequences are fused to the 3'-end of DsRed2. DsRed2 is a human codon-optimized variant of wild-type DsRed that has been engineered for faster maturation and lower non-specific aggregation. pDsRed2-Nuc is designed for fluorescent labeling of the nucleus in living cells.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications. AcGFP1 and DsRed2 protein fusions were transiently transfected and visualized by fluorescence microscopy. Panel A. pAcGFP1-Mito (mitochondria) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Panel B. pAcGFP1-Golgi (Golgi apparatus) and pDsRed2-Nuc (nucleus) in HEK 293 cells.

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Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors.

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Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.

632409 pDsRed2-ER Vector 20 ug USD $615.00

pDsRed2-ER is a mammalian expression vector designed to label the endoplasmic reticulum in living cells. The vector encodes a fusion consisting of Discosoma sp. red fluorescent protein (DsRed2); the endoplasmic reticulum (ER) targeting sequence of calreticulin, fused to the 5' end of DsRed2; and the ER retention sequence, KDEL, fused to the 3' end of DsRed2. DsRed2 is a human codon-optimized variant of wild-type DsRed that has been engineered for faster maturation and lower non-specific aggregation.

pDsRed2-ER can be introduced into mammalian cells using any standard transfection method. If required, stable transformants can be selected using G418.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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632409: pDsRed2-ER Vector

632409: pDsRed2-ER Vector

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AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications. AcGFP1 and DsRed2 protein fusions were transiently transfected and visualized by fluorescence microscopy. Panel A. pAcGFP1-Mito (mitochondria) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Panel B. pAcGFP1-Golgi (Golgi apparatus) and pDsRed2-Nuc (nucleus) in HEK 293 cells.

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Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors.

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Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.

632418 pDsRed2-Peroxi Vector 20 ug USD $615.00

pDsRed2-Peroxi encodes a fusion of Discosoma sp. red fluorescent protein (DsRed2) and the peroxisomal targeting signal 1 (PTS1). The PTS1 sequence is fused to the 3'-end of DsRed2 and encodes the tripeptide SKL, which targets the fusion protein to the matrix of peroxisomes. DsRed2 is human codon-optimized for high expression in mammalian cells. pDsRed2-Peroxi is designed for fluorescent labeling of peroxisomes.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications. AcGFP1 and DsRed2 protein fusions were transiently transfected and visualized by fluorescence microscopy. Panel A. pAcGFP1-Mito (mitochondria) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Panel B. pAcGFP1-Golgi (Golgi apparatus) and pDsRed2-Nuc (nucleus) in HEK 293 cells.

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Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors.

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Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.

632419 pDsRed2-Bid Vector 20 ug USD $615.00

pDsRed2-Bid encodes a fusion of Discosoma sp. red fluorescent protein (DsRed2) and Bid, a member of the Bcl-2 family. In healthy, non-apoptotic cells, Bid resides in the cytosol as soluble protein. Upon induction of apoptosis, Bid translocates to mitochondria. In cells expressing the Bid-DsRed2 fusion, the translocation can be detected by fluorescence microscopy.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Monitoring Bid activation with the pDsRed2-Bid Vector

Monitoring Bid activation with the pDsRed2-Bid Vector

Monitoring Bid activation with the pDsRed2-Bid Vector. 3T3 cells were transiently transfected with pDsRed2-Bid (Panels A & B). Approximately 24 hr later, cells were incubated with 700 nM staurosporine, an apoptosis inducing agent, at 37°C for 3 hr. Cells were then fixed and examined with a Zeiss Axioskop equipped with the appropriate light filters. In untreated cells (Panel A), the Bid reporter (Bid-DsRed2) distributes uniformly in the cytosol, as expected. Following induction (Panel B), the reporter translocates to the surface of mitochondria, forming intensely fluorescent clusters—clear evidence that the Bid pathway has been activated.

632420 pIRES2-DsRed2 Vector 20 ug USD $615.00

Bicistronic expression vector containing DsRed2 as a marker for transfection efficiency. A gene of interest is inserted into the MCS located upstream of the encephalomyocarditis virus (EMCV) internal ribosomal entry site (IRES). The IRES sequence allows both the gene of interest and DsRed2 to be translated simultaneously from the same mRNA transcript. DsRed2 is a human codon-optimized variant of wild-type DsRed that has been engineered for faster maturation and lower non-specific aggregation.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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632420: pIRES2-DsRed2 Vector

632420: pIRES2-DsRed2 Vector

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Map of the fluorescent pIRES2 bicistronic expression vectors

Map of the fluorescent pIRES2 bicistronic expression vectors
Map of the fluorescent pIRES2 bicistronic expression vectors.

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Expression of two proteins from a single mRNA transcript

Expression of two proteins from a single mRNA transcript
Expression of two proteins from a single mRNA transcript. A fluorescent protein is translated from an internal ribosome entry site (IRES).
632421 pDsRed2-Mito Vector 20 ug USD $615.00

pDsRed2-Mito encodes a fusion of Discosoma sp. red fluorescent protein (DsRed2) and a mitochondrial targeting sequence of human cytochrome c oxidase subunit VIII (Mito). The targeting sequence is fused to the 5'-end of DsRed2, which is human codon-optimized for high expression in mammalian cells. pDsRed2-Mito is designed for fluorescent labeling of mitochondria.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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632421: pDsRed2-Mito Vector

632421: pDsRed2-Mito Vector

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AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications

AcGFP1 is ideal for multicolor and fluorescence microscopy applications. AcGFP1 and DsRed2 protein fusions were transiently transfected and visualized by fluorescence microscopy. Panel A. pAcGFP1-Mito (mitochondria) and pDsRed2-Nuc (nucleus) in HEK 293 cells. Panel B. pAcGFP1-Golgi (Golgi apparatus) and pDsRed2-Nuc (nucleus) in HEK 293 cells.

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Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors

Organelles targeted by the subcellular localization vectors.

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Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure

Subcellular localization vectors encode fusions of fluorescent proteins with localization signals or subcellular structural proteins, which target the fluorescent protein to a specific organelle or subcellular structure. The vectors are available in a variety of organelle- and cytoskeleton-targeted color variants.

mCherry antibody, TdTomato antibody, DsRed antibody Red fluorescent protein antibodies
Selection guides Fluorescent protein quick guide

Overview

  • DsRed2 excitation and emission maxima are 563 and 582 nm, respectively
  • High solubility and low aggregation
  • DsRed-Express2 is an alternative DsRed version with mutations that enhance its solubility, reduce its green emission, and accelerate its maturation

More Information

Applications

  • Monitoring transfection efficiency
  • Protein localization studies
  • Multicolor experiments with AcGFP1 or other fluorescent proteins
References

Haas, J., Park, E.-C. & Seed, B. Codon usage limitation in the expression of HIV-1 envelope glycoprotein. Curr. Biol. 6, 315–324 (1996).

Matz, M. V et al. Fluorescent proteins from nonbioluminescent Anthozoa species. Nat. Biotechnol. 17, 969–973 (1999).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

 


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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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