mBanana fluorescent protein

mBanana yellow fluorescent protein vectors

mBanana is a fruit fluorescent protein which was developed in Dr. Roger Tsien’s lab (Shaner et al, 2004; Wang et al, 2004; Shu et al, 2006) by directed mutagenesis of mRFP1, a monomeric mutant of DsRed (Campbell et al, 2002). Fruit fluorescent proteins such as mBanana have demonstrated stable expression and perform successfully in numerous fusion applications. We offer monoclonal and polyclonal antibodies to detect mBanana.

Cat. #	Product	Size	Price	License	Quantity	Details
632528	pmBanana Vector	20 ug	Inquire for Quotation		*
pmBanana encodes mBanana, a mutant derived from the tetrameric Discosoma sp. red fluorescent protein, DsRed. In this vector, the mBanana coding sequence is flanked by MCS regions, making it easy to excise the gene for use in other cloning applications. pmBanana is primarily intended to serve as a source of mBanana cDNA. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back 632528: pmBanana Vector Back Basic fluorescent protein vector map Basic fluorescent protein vector map. Use this bacterial expression vector as a source of the fluorescent protein gene. Note: There is a stop codon at the 5' end of the 3' MCS. The 3' MCS should not be used for cloning.

*You must be logged in to a Purchasing Account in order to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@takarabio.com.

Overview

Bright yellow fluorescent protein
mBanana excitation and emission maxima are 540 and 553 nm, respectively

More Information

Applications

Fusions
Protein localization
General reporter for mammalian cells
Monitoring transfection efficiencies

References

Shaner, N. C. et al. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature Biotechnol. 22(12):1567–1572 (2004).

Wang, L. et al. Evolution of new nonantibody proteins via iterative somatic hypermutation. Proc. Nat. Acad. Sci. USA. 101(48):16745–16749 (2004).

Shu, X. et al. Novel Chromophores and Buried Charges Control Color in mFruits. Biochemistry 45(32):9639–9647 (2006).

Campbell, R. E. et al. A monomeric red fluorescent protein. Proc. Nat. Acad. Sci. USA. 99(12):7877–7882 (2002).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Fluorescent protein related links

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