High-throughput isolation of cell-free DNA from plasma

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Genomic DNA purification

Product overview

The NucleoMag cfDNA kit is designed for the efficient isolation of circulating cell-free DNA (cfDNA) from human blood plasma. Fragmented DNA as small as 50 bp can be purified at high yield due to a reversible adsorption of nucleic acids to paramagnetic beads under specialized buffer conditions. The yield can vary depending on the sample and is typically in the range of 0.1 to 100 ng DNA per ml of plasma. The purification procedure takes less than an hour for 24 preps.

Consistent cfDNA recovery from 1–10 ml of plasma
Efficient purification of fragmented DNA as small as 50 bp
No PCR inhibition
Manual or high-throughput automated isolation of circulating cell-free DNA from human plasma using EDTA or Cell-Free DNA BCT tubes
Typical downstream applications: NGS, qPCR, ddPCR

At-a-glance Higher yield Highly consistent Expected yield Protocol schematic

At-a-glance

Technology	Magnetic bead technology
Format	Highly reactive superparamagnetic beads
Processing	Manual or automated
Starting material	1–10 ml human EDTA/Cell-Free DNA BCT plasma
Fragment size	>50 bp
Typical yield	Depends on sample source, storage, and quality
A₂₆₀/A₂₈₀	1.6–1.9
Elution volume	50-200 µl
Preparation time	55 min/24 preps (2 ml; excluding lysis)*
Binding capacity	0.3 µg/µl beads

* Using a KingFisher Flex

Higher yield

Highly consistent

Consistent recovery of cfDNA regardless of plasma volume.

Expected yield

DNA yield ranges from 0.1 to 100 ng DNA per ml plasma.

Protocol schematic

Related Products

Cat. #	Product	Size	Price
744550.1	NucleoMag® cfDNA	48 Preps	USD $423.00
The NucleoMag cfDNA kit provides an efficient, robust method for high-throughput isolation of circulating cell-free DNA (cfDNA) from 1–10 ml of human plasma obtained using EDTA or Cell-Free DNA BCT collection tubes. The kit enables recovery of fragmented DNA ≥50 bp in size via reversible adsorption of nucleic acids to paramagnetic beads under optimized buffer conditions, and eluted cfDNA is ready for downstream applications such as qPCR, ddPCR, NGS, etc. NucleoMag cfDNA is compatible with manual or automated processing and enables preparation of 24 samples in less than an hour (excluding cell lysis) on the KingFisher Flex platform. Cat. # 744550.1 includes sufficient quantities of reagents and materials for processing 48 samples. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back The NucleoMag cfDNA procedure The NucleoMag cfDNA procedure. Sample lysis is achieved by incubation of plasma with Liquid Proteinase K and Lysis Buffer MCF1 at 56°C. Buffer MCF2 and paramagnetic NucleoMag P-Beads are then added, enabling optimal binding of nucleic acids. Three washing steps efficiently remove contaminating substances, such as PCR inhibitors, after magnetic separation. Residual ethanol is removed by a drying step, and highly pure DNA is eluted from the paramagnetic beads with 50–200 µl of Elution Buffer MCF5—a slightly alkaline buffer of low ionic strength (5 mM Tris/HCl, pH 8.5). The NucleoMag cfDNA kit can be used either manually or on automated liquid handling platforms or magnetic separators. Back Reliable purification of cfDNA from different volumes of plasma Reliable purification of cfDNA from different volumes of plasma. The NucleoMag cfDNA (formerly known as NucleoMag DNA Plasma) kit was used to isolate cfDNA from 2–10 ml inputs of EDTA-treated human plasma. Analysis of purified cfDNA on the Agilent Bioanalyzer 2100 system using the High Sensitivity DNA Kit yielded a characteristic peak at 170 bp for each input volume. The total yield from each sample was determined using the PicoGreen quantification assay with the Quant-iT PicoGreen kit on a Synergy HT Multi-Detection Microplate Reader (Biotek). The consistent correlation observed between cfDNA yield and input volume demonstrates the reliable performance of the NucleoMag cfDNA kit. Back Efficient cfDNA recovery regardless of input volume Efficient cfDNA recovery regardless of input volume. cfDNA isolated from varying input amounts of EDTA-treated human plasma using the NucleoMag cfDNA kit yielded clear bands upon measurement by capillary electrophoresis with the Agilent Bioanalyzer 2100 system and the High Sensitivity DNA Kit. Even for volumes as low as 2 ml, purified cfDNA was clearly visible. Back Higher, more consistent yields relative to a competitor kit Higher, more consistent yields relative to a competitor kit. Total cfDNA was purified from 2-ml inputs of EDTA-treated human plasma from four challenging donor samples containing low levels of cfDNA (<10 ng cfDNA/ml of plasma) using NucleoMag cfDNA (formerly known as NucleoMag DNA Plasma) and a kit from Competitor T. Total DNA recovery was quantified using the Qubit dsDNA High Sensitivity kit on a Qubit fluorometer. Relative to the kit from Competitor T, NucleoMag cfDNA provided higher yields with lower sample-to-sample variability.
744550.4	NucleoMag® cfDNA	4 x 48 Preps	USD $1530.00
The NucleoMag cfDNA kit provides an efficient, robust method for high-throughput isolation of circulating cell-free DNA (cfDNA) from 1–10 ml of human plasma obtained using methods such as EDTA or Cell-Free DNA BCT collection tubes. The kit enables recovery of fragmented DNA ≥50 bp in size via reversible adsorption of nucleic acids to paramagnetic beads under optimized buffer conditions. The eluted cfDNA is ready for downstream applications such as qPCR, ddPCR, NGS, etc. NucleoMag cfDNA is compatible with manual or automated processing, and enables preparation of 24 samples in less than an hour (excluding cell lysis) on the KingFisher Flex platform. Cat. # 744550.4 includes sufficient quantities of reagents and materials for processing 192 samples. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back The NucleoMag cfDNA procedure The NucleoMag cfDNA procedure. Sample lysis is achieved by incubation of plasma with Liquid Proteinase K and Lysis Buffer MCF1 at 56°C. Buffer MCF2 and paramagnetic NucleoMag P-Beads are then added, enabling optimal binding of nucleic acids. Three washing steps efficiently remove contaminating substances, such as PCR inhibitors, after magnetic separation. Residual ethanol is removed by a drying step, and highly pure DNA is eluted from the paramagnetic beads with 50–200 µl of Elution Buffer MCF5—a slightly alkaline buffer of low ionic strength (5 mM Tris/HCl, pH 8.5). The NucleoMag cfDNA kit can be used either manually or on automated liquid handling platforms or magnetic separators. Back Reliable purification of cfDNA from different volumes of plasma Reliable purification of cfDNA from different volumes of plasma. The NucleoMag cfDNA (formerly known as NucleoMag DNA Plasma) kit was used to isolate cfDNA from 2–10 ml inputs of EDTA-treated human plasma. Analysis of purified cfDNA on the Agilent Bioanalyzer 2100 system using the High Sensitivity DNA Kit yielded a characteristic peak at 170 bp for each input volume. The total yield from each sample was determined using the PicoGreen quantification assay with the Quant-iT PicoGreen kit on a Synergy HT Multi-Detection Microplate Reader (Biotek). The consistent correlation observed between cfDNA yield and input volume demonstrates the reliable performance of the NucleoMag cfDNA kit. Back Efficient cfDNA recovery regardless of input volume Efficient cfDNA recovery regardless of input volume. cfDNA isolated from varying input amounts of EDTA-treated human plasma using the NucleoMag cfDNA kit yielded clear bands upon measurement by capillary electrophoresis with the Agilent Bioanalyzer 2100 system and the High Sensitivity DNA Kit. Even for volumes as low as 2 ml, purified cfDNA was clearly visible. Back Higher, more consistent yields relative to a competitor kit Higher, more consistent yields relative to a competitor kit. Total cfDNA was purified from 2-ml inputs of EDTA-treated human plasma from four challenging donor samples containing low levels of cfDNA (<10 ng cfDNA/ml of plasma) using NucleoMag cfDNA (formerly known as NucleoMag DNA Plasma) and a kit from Competitor T. Total DNA recovery was quantified using the Qubit dsDNA High Sensitivity kit on a Qubit fluorometer. Relative to the kit from Competitor T, NucleoMag cfDNA provided higher yields with lower sample-to-sample variability.
744903	NucleoMag® SEP 24	Each	USD $835.00
The NucleoMag SEP 24 magnetic separator is designed for the rapid manual purification of nucleic acids using the NucleoMag kits. NucleoMag kits employ Macherey-Nagel's magnetic bead-based sample processing technology, and enable rapid purification of high-quality nucleic acids from multiple samples in parallel. The NucleoMag SEP 24 (Cat. # 744903) is used with 24-well plates. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back 744903: NucleoMag SEP 24
744902	NucleoMag® SEP Maxi	Each	USD $306.00
The NucleoMag SEP Maxi magnetic separator is designed for the rapid manual purification of nucleic acids using the NucleoMag kits. NucleoMag kits employ Macherey-Nagel's magnetic bead-based sample processing technology, and enable rapid purification of high-quality nucleic acids from multiple samples in parallel. The NucleoMag SEP Maxi (Cat. # 744902) is used with 50-ml tubes and carries 4 tube positions. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back NucleoMag SEP Maxi: magnetic separator for use with 50-ml tubes NucleoMag SEP Maxi: magnetic separator for use with 50-ml tubes. Back 744902: NucleoMag SEP Maxi

Cell-free DNA-seq library prep in under 2 hours

ThruPLEX Plasma-Seq Kit

Efficient library preparation from as little as 1 ng of cell-free DNA
Excellent complexity and GC representation
Three-step, single-tube protocol can be completed in less than 2 hours
UDIs or dual index options for multiplexing up to 384 samples

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Product overview

At-a-glance

Higher yield

Highly consistent

Expected yield

Protocol schematic

Related Products

Notice to purchaser

The NucleoMag cfDNA procedure

Reliable purification of cfDNA from different volumes of plasma

Efficient cfDNA recovery regardless of input volume

Higher, more consistent yields relative to a competitor kit

Notice to purchaser

The NucleoMag cfDNA procedure

Reliable purification of cfDNA from different volumes of plasma

Efficient cfDNA recovery regardless of input volume

Higher, more consistent yields relative to a competitor kit

Notice to purchaser

744903: NucleoMag SEP 24

Notice to purchaser

NucleoMag SEP Maxi: magnetic separator for use with 50-ml tubes

744902: NucleoMag SEP Maxi

ThruPLEX Plasma-Seq Kit