Antibodies to detect GFP, ZsGreen1, AcGFP1, or EGFP

Monoclonal and polyclonal antibodies to detect green fluorescent proteins.

We offer a wide selection of antibodies to detect green fluorescent proteins including monoclonal and polyclonal antibodies. For more info about which fluorescent protein(s) can be detected with each antibody, please refer to the table in the overview section below.

Cat. #	Product	Size	Quantity
632598	Living Colors® ZsGreen Monoclonal Antibody	100 uL	*
The Living Colors ZsGreen Monoclonal Antibody was raised in mouse against recombinant full-length Zoanthus sp. green fluorescent protein (ZsGreen), and Protein A-purified. In Western blot applications, it specifically recognizes ZsGreen and does not cross-react with AcGFP1, AmCyan, ZsYellow, DsRed-Express, mCherry, tdTomato, Dendra2, or DsRed2. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back 632598: Living Colors ZsGreen Monoclonal Antibody
632592	Living Colors® Full-Length GFP Polyclonal Antibody	100 uL	*
The Living Colors Full-Length GFP Polyclonal Antibody was raised in a rabbit against recombinant full-length Aequorea coerulescens green fluorescent protein (rAcGFP1). This antibody recognizes native and denatured forms of AcGFP1 and GFP, as well as N- or C-terminal fusion proteins of AcGFP1 expressed in mammalian cells. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 632592: Living Colors Full-Length GFP Polyclonal Antibody Back The new Full-Length GFP Polyclonal Antibody provides equivalent performance to our previous Full-Length A.v. Polyclonal Antibody The new Full-Length GFP Polyclonal Antibody provides equivalent performance to our previous Full-Length A.v. Polyclonal Antibody. The Full-Length GFP Polyclonal Antibody detects AcGFP1, wild type GFP, and GFPuv. Based on their homology to GFP, we also expect this antibody to detect EGFP, EYFP, and ECFP. Lanes 1. 5 ng rAcGFP1. Lanes 2. 2 ng rAcGFP1. Lanes 3.0.5 ng rAcGFP1. Lanes 4.0.2 ng rAcGFP1. Lanes 5.0.1 ng rAcGFP1.
632569	Living Colors® EGFP Monoclonal Antibody	100 uL	*
A monoclonal antibody produced by hybridoma cells raised against full-length enhanced green fluorescent protein (EGFP). This antibody recognizes native and denatured forms of EGFP, destabilized EGFP variants, ECFP, EYFP, EBFP, wild-type GFP, GFPuv, and AcGFP in bacterial and mammalian cell lysates. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Living Colors Fluorescent Protein antibody sources and dilutions for western blot, immunoprecipitation (IP) and immunocytochemistry (IC) Living Colors Fluorescent Protein antibody sources and dilutions for western blot, immunoprecipitation (IP) and immunocytochemistry (IC). Back The EGFP Monoclonal Antibody, for the detection of enhanced GFP The EGFP Monoclonal Antibody, for the detection of enhanced GFP. Western blot using the Living Colors EGFP Monoclonal Antibody at a 1:8,000 dilution. Lane 1. 50 ng recombinant EGFP. Lanes 2–3. Lysates from HeLa cells expressing either a negative control (Lane 2), an EGFP fusion protein (Lane 3).
632474	Living Colors® Full-Length ZsGreen Polyclonal Antibody	100 uL	*
The Living Colors Full-Length ZsGreen Polyclonal Antibody was raised in a rabbit against recombinant full-length Zoanthus sp. green fluorescent protein (ZsGreen). This antibody recognizes native and denatured forms of ZsGreen as well as fusion proteins containing ZsGreen in bacterial and mammalian cell lysates. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Living Colors Fluorescent Protein antibody sources and dilutions for western blot, immunoprecipitation (IP) and immunocytochemistry (IC) Living Colors Fluorescent Protein antibody sources and dilutions for western blot, immunoprecipitation (IP) and immunocytochemistry (IC). Back 632474: Living Colors Full-Length ZsGreen Polyclonal Antibody
632381	Living Colors® A.v. Monoclonal Antibody (JL-8)	200 uL	*
A monoclonal antibody produced by hybridoma cells against full-length Aequorea victoria green fluorescent protein (GFP). This antibody recognizes native and denatured forms of wild-type GFP, GFPuv, AcGFP, EGFP, destabilized EGFP variants, EBFP, EYFP, ECFP, AcGFP, and both N- and C-terminal fusion proteins containing these GFP variants in bacterial and mammalian cell lysates. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Living Colors Fluorescent Protein antibody sources and dilutions for western blot, immunoprecipitation (IP) and immunocytochemistry (IC) Living Colors Fluorescent Protein antibody sources and dilutions for western blot, immunoprecipitation (IP) and immunocytochemistry (IC).

Overview

Living Colors antibodies are specific for individual green fluorescent proteins, including:

- Aequorea victoria GFP variants: EGFP, wild-type GFP, GFPuv
- Aequorea coerulescens GFP variant: AcGFP1
- Zoanthus sp. variant: ZsGreen1
Used for immunoprecipitation and immunolabeling applications in numerous studies

Fluorescent protein	Recommended antibody	Cat. #s.	Proven uses*
AcGFP1	A.v. Monoclonal Antibody (JL-8)	632381	WB, IP, IL
AcGFP1	Full Length GFP Polyclonal Antibody	632592	WB, IP, IL
EGFP	EGFP Monoclonal Antibody	632569	WB, IP, IL
EGFP	Full-Length GFP Polyclonal Antibody	632592	WB, IP, IL
GFP & GFPuv	Full Length GFP Polyclonal Antibody	632592	WB, IL
ZsGreen1	Full-Length ZsGreen Monoclonal Antibody	632598	WB
ZsGreen1	Full Length ZsGreen1 Polyclonal Antibody	632474	WB, IP, IL

*WB = Western Blot, IP = immunoprecipitation, IL = immunolabeling

More Information

Applications

Western blot
Immunoprecipitation
Immunocytochemistry
Immunohistochemistry

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Fluorescent protein related links

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Antibodies and ELISA

Antibodies to detect GFP, ZsGreen1, AcGFP1, or EGFP

Notice to purchaser

632598: Living Colors ZsGreen Monoclonal Antibody

Notice to purchaser

632592: Living Colors Full-Length GFP Polyclonal Antibody

The new Full-Length GFP Polyclonal Antibody provides equivalent performance to our previous Full-Length A.v. Polyclonal Antibody

Notice to purchaser

Living Colors Fluorescent Protein antibody sources and dilutions for western blot, immunoprecipitation (IP) and immunocytochemistry (IC)

The EGFP Monoclonal Antibody, for the detection of enhanced GFP

Notice to purchaser

Living Colors Fluorescent Protein antibody sources and dilutions for western blot, immunoprecipitation (IP) and immunocytochemistry (IC)

632474: Living Colors Full-Length ZsGreen Polyclonal Antibody

Notice to purchaser

Living Colors Fluorescent Protein antibody sources and dilutions for western blot, immunoprecipitation (IP) and immunocytochemistry (IC)

Overview

More Information

Applications

Additional product information

Fluorescent protein related links