Antibodies to detect orange fluorescent proteins: mOrange2

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Living Colors Fluorescent Protein antibody sources and dilutions for western blot, immunoprecipitation (IP) and immunocytochemistry (IC)

Living Colors Fluorescent Protein antibody sources and dilutions for western blot, immunoprecipitation (IP) and immunocytochemistry (IC).

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The mCherry monoclonal antibody detects PAmCherry in the lysate of mammalian cells

The mCherry monoclonal antibody detects PAmCherry-N1 in the lysate of mammalian cells. HEK 293 cells were transiently transfected with pPAmCherry-N1. Cell lysates (corresponding to 30,000 cells) were prepared from HEK 293 cells transiently expressing PAmCherry-N1 (Lane 1) or a negative control (untransfected cells; Lane 2). Both lysates and a positive control (5 ng recombinant mCherry; Lane 3) were separated by SDS-PAGE and analyzed by Western blot using the mCherry monoclonal antibody at the recommended dilution of 1:1000.

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The mCherry Monoclonal Antibody differentiates red fluorescent proteins

The mCherry Monoclonal Antibody differentiates red fluorescent proteins. HEK293 cells were transiently transfected with vectors encoding the following fluorescent proteins, and then analyzed by Western blot with the mCherry Monoclonal Antibody (0.5 mg/ml; 1:1000 dilution). Panel A. The mCherry Monoclonal Antibody recognizes mCherry but not tdTomato, DsRed-Express2, or DsRed-Monomer. Lane 1: control (untransfected cells), Lane 2: mCherry, Lane 3: tdTomato, Lane 4: DsRed-Monomer, Lane 5: DsRed-Express2. Panel B. The mCherry Monoclonal Antibody differentiates some Fruit Fluorescent Proteins. It detects mCherry, mOrange, mPlum, and mStrawberry, but not mRaspberry or tdTomato. mBanana is only faintly detected. Lane 1: control (untransfected cells), Lane 2: mCherry, Lane 3: mBanana, Lane 4: mOrange, Lane 5: mPlum, Lane 6: mRaspberry, Lane 7: mStrawberry, Lane 8: tdTomato. Exposure times, 15 sec and 5 min, respectively. Lanes M, marker.

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632543: Living Colors mCherry Monoclonal Antibody