Peptide Coating Kit
Generally, low molecular weight peptides and synthetic peptides do not spontaneously attach to 96-well microtiter ELISA plates. The Peptide Coating Kit is designed to allow optimal peptide plate coating onto 96-well plates. The Peptide Coating Kit also includes reaction buffer, 96-well microplates, and blocking solution designed specifically for subsequent ELISA.
The free carboxyl groups of peptides used for plate coating can be covalently linked via reaction with the water-soluble peptide coupling reagent l-ethyl-3-(3-(dimethylamino)-propy1)carbodiimide (EDC). These peptides can subsequently be linked to amino groups that are attached to the surface of microtiter plate wells. The end result is a population of peptides directly coating the surface of the microtiter plate via peptide bonds.
- Detection of antigen via ELISA
- Detection of antibody via competitive ELISA
|Reaction plate||96-well (8 well × 12 strips) × 5 plates|
|Coupling reagent||1 vial (for 5 ml × 1)|
|Reaction buffer||1 vial (50 ml × 1)|
|Blocking solution||2 vials (50 ml × 2)|
Nakajima, N. & Ikada, Y. Mechanism of amide formation by carbodiimide for bioconjugation in aqueous media. Bioconjug. Chem. 6, 123–130 (1995).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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