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  • ‹ Back to Bone research
  • Bone specific alkaline phosphatase
  • Collagen type 2
  • Dentin matrix protein 1
  • Osteocalcin (bovine)
  • Osteocalcin (human)
  • Osteocalcin (mouse)
  • Osteocalcin (pig)
  • Osteocalcin (rat)
  • Osteocalcin carboxylated Gla-OC
  • Osteocalcin undercarboxylated Glu-OC
  • Osteonectin
  • Procollagen type I C-peptide
  • Tartrate-resistant acid phosphatase (TRACP)
Home › Products › Antibodies and ELISA › Primary antibodies and ELISAs by research area › Bone research › Osteocalcin (rat)

Primary antibodies and ELISAs by research area

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Osteocalcin (rat) detection

Osteocalcin, which contains 2–3 gamma-carboxyglutamate (Gla) residues and has a molecular weight of approximately 5.9 kDa, is known as a vitamin K-dependent, calcium-binding, non-collagenous protein. Produced only by osteoblasts, osteocalcin has been used as a unique marker of bone formation and resorption. The rat osteocalcin protein consists of 50 amino acids, while human, bovine, rabbit, and other species express an osteocalcin protein containing 49 amino acids.

Osteocalcin, which contains 2–3 gamma-carboxyglutamate (Gla) residues and has a molecular weight of approximately 5.9 kDa, is known as a vitamin K-dependent, calcium-binding, non-collagenous protein. Produced only by osteoblasts, osteocalcin has been used as a unique marker of bone formation and resorption. The rat osteocalcin protein consists of 50 amino acids, while human, bovine, rabbit, and other species express an osteocalcin protein containing 49 amino acids. Osteocalcin is also a hormone that plays a role in insulin regulation and male fertility. Meanwhile, the undercarboxylated form of osteocalcin (ucOC) is responsible for glucose metabolism. The Gla-osteocalcin and Glu-osteocalcin ELISA kits enable researchers to study the roles of both carboxylated and undercarboxylated osteocalcin in metabolic disease, male fertility, and bone formation and resorption.

Bone formation and resorption require the action of osteoblasts, which produce osteocalcin exclusively. Osteocalcin is typically produced with all three of its glutamate residues carboxylated (Gla-OC), affording the protein the ability to form a calcium pocket and bind bone matrix. The carboxylated glutamate residues are at positions 17, 21, and 24 of the protein. During bone metabolism, osteocalcin is released from bone matrix through the action of various enzymes, including one produced by osteoclasts. Most of the three glutamate residues are decarboxylated on osteocalcin (Glu-OC) when it is released into bloodstream from bone. Thus, osteocalcin is present in blood in both its Gla and Glu forms.

Alternate names: bone gamma-carboxyglutamic acid-containing protein, BGLAP

ELISA kits for osteocalcin (rat) detection

The Rat Gla-Osteocalcin High Sensitive EIA Kit (Cat. # MK126) is a 96-well, sandwich-type EIA that takes advantage of a C-terminal rat osteocalcin monoclonal antibody to capture osteocalcin (OC) in rat biological samples. This antibody has minimal cross-reactivity to bovine, human, and rabbit osteocalcin. An enzyme-labeled antibody (Gla-OC4-30) specific to Gla-OC is used as the detection antibody, allowing this kit to detect Gla-osteocalcin with very high sensitivity. As such, this EIA kit is sensitive enough to pick up even minute traces of rat Gla-osteocalcin in cell supernatants supplemented with fetal calf serum.

The Rat Glu-Osteocalcin High Sensitive EIA Kit (Cat. # MK146) is a 96-well, sandwich-type EIA that uses a C-terminal rat osteocalcin monoclonal antibody to capture osteocalcin (OC). This kit's detection antibody specifically recognizes the glutamate residues present at positions 21 and 24 of the protein, making highly sensitive yet reproducible measurements of low-level Glu-OC possible. Moreover, because both the Rat Gla-Osteocalcin High Sensitive EIA Kit (Cat. # MK126) and the Rat Glu-Osteocalcin High Sensitive EIA Kit (Cat. # MK146) contain the same capture antibody, both kits may be used for Gla/Glu detection (Cat. # MK147), making simultaneous monitoring of bone formation and resorption possible.

Antibodies for osteocalcin (rat) detection

Monoclonal Anti-Rat Osteocalcin (Clone D-8G, Clone 6-7H, Clone 9-12H) antibodies selectively detect rat osteocalcin. Cat. # M185 and Cat. # M186 may be used for osteocalcin immunohistochemistry (IHC) with formalin-fixed and paraffin-embedded tissues, while Cat. # M186 and Cat. # M187 may be used for Western blot (WB) analysis.

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Cat. # Product Size License Quantity Details
MK147 Gla/Glu Osteocalcin High Sensitive EIA Set (Rat) Each USD $1245.00

Gla/Glu Osteocalcin High Sensitive EIA Set (Rat; Cat. # MK147) is a combination of Rat Gla-Osteocalcin High Sensitive EIA Kit (Cat.# MK126) and Rat Glu-Osteocalcin High Sensitive EIA Kit (Cat.# MK146). Since both kits have the same capture antibody, they can be used together for simultaneous Gla/Glu detection, thereby making simultaneous monitoring of bone formation and resorption possible.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents
MK146 Rat Glu-Osteocalcin High Sensitive EIA Kit 96 Rxns USD $817.00

Rat Glu-Osteocalcin High Sensitive EIA Kit is a sandwich-type EIA kit in which rat osteocalcin C terminal region recognition specific antibody is the capture antibody on a solid plate and a monoclonal antibody that is specific to the Glu residues that straddle positions 21 and 24 of osteocalcin is arranged as the detection antibody. This product makes high-sensitivity measurement of minor antigens and maintenance of stable reproducibility possible. The use of a 96-well plate makes it possible to assay many sample treatments. Moreover, as it has the same capture antibody as the Rat Gla-Osteocalcin High Sensitive EIA Kit (Cat. #MK126), the kits may be used together for simultaneous Gla/Glu detection, thereby making simultaneous monitoring of bone formation and resorption possible.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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MK146: Rat Glu-Osteocalcin High Sensitive EIA Kit

MK146: Rat Glu-Osteocalcin High Sensitive EIA Kit
MK126 Rat Gla-Osteocalcin High Sensitive EIA Kit 96 Rxns USD $817.00

Rat Gla-Osteocalcin High Sensitive EIA Kit is a sandwich-type EIA kit which uses a rat osteocalcin C-terminus-specific antibody as capture-antibody on a solid-phase plate. This antibody has a minimal cross reactivity with bovine, human and rabbit osteocalcin. An enzyme-labeled antibody (GlaOC4-30) specific to Gla-OC is used as the detection antibody, allowing this kit to detect Gla-osteocalcin with a very high sensitivity. As such, this EIA kit is sensitive enough to detect even minute levels of rat osteocalcin produced in supernatants of cells cultured in fetal calf serum-supplemented medium.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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MK126: Rat Gla-Osteocalcin High Sensitive EIA Kit

MK126: Rat Gla-Osteocalcin High Sensitive EIA Kit
M187 Monoclonal Anti-Rat Osteocalcin (Clone 9-12H) 0.1 mg USD $457.00

License Statement

ID Number  
M76 This product is covered by the claims of Japanese Patent No. 5280916.

This monoclonal antibody was obtained by fusing the mouse myeloma cell-line P3U1 with spleen cells from a BALB/c mouse after immunization with a KLH-conjuagated peptide from rat osteocalcin (amino acids 38–50: FQDAYKRIYGTTV). The monoclonal antibody was harvested from ascitic fluid.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components
M186 Monoclonal Anti-Rat Osteocalcin (Clone 6-7H) 0.1 mg USD $457.00

This monoclonal antibody was obtained by fusing the mouse myeloma cell-line P3U1 with spleen cells from a BALB/c mouse after immunization with a KLH-conjugated peptide from rat osteocalcin (amino acids 1–25: YLNNGLGAPAPYPDPLEPHREVCEL). The monoclonal antibody was harvested from ascitic fluid.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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M186: Anti-Rat Osteocalcin, Monoclonal (Clone 6-7H)

M186: Anti-Rat Osteocalcin, Monoclonal (Clone 6-7H)
M185 Monoclonal Anti-Rat Osteocalcin (Clone D-8G) 0.1 mg USD $457.00

This monoclonal antibody was obtained by fusing the mouse myeloma cell line P3U1 with spleen cells from a BALB/c mouse after immunization with a KLH-conjugated peptide from rat osteocalcin (amino acids 1–25: YLNNGLGAPAPYPDPLEPHREVCEL). The monoclonal antibody was harvested from ascitic fluid.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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M185: Anti-Rat Osteocalcin, Monoclonal (Clone D-8G)

M185: Anti-Rat Osteocalcin, Monoclonal (Clone D-8G)

Overview

Antigen/peptide
Cat. #
Application
Species
(Clone) and source
Cross‑reactivity
Gla-Osteocalcin (C-terminus) MK126 ELISA Rat Capture:
(n/a)
MoAb

Detect: HRP
(GlaOC4-30) MoAb
Minimal cross‑reactivity with bovine, human, and rabbit osteocalcin
Glu-Osteocalcin (C-terminus) MK146 ELISA Rat Capture:
(n/a)
MoAb

Detect: HRP
(n/a)
MoAb

Detection antibody epitope: Glu residues that straddle positions 21 and 24 of osteocalcin
Does not cross-react with bovine, human, horse, dog, pig, sheep, goat, rabbit, or guinea pig osteocalcin
Gla/Glu-Osteocalcin
(C-terminus)
MK147 (MK126 +MK146) ELISA Rat See Clone and Source information for MK126 and MK146, above. Minimal cross-reactivity with bovine, human, and rabbit osteocalcin
Osteocalcin
(1–25) conjugated to KLH
M185 IHC Rat (D-8G)
BALB/c Mouse IgG1 MoAb
Cross‑reacts with bovine, pig, goat, sheep, and donkey osteocalcin

Does not react with human osteocalcin
Osteocalcin
(1–25) conjugated to KLH
M186 IHC
WB

Rat (6–7H)
BALB/c Mouse IgG1 MoAb
Cross-reacts with bovine osteocalcin

Does not react with human osteocalcin
Osteocalcin
(38–50) conjugated to KLH
M187 WB Rat (9–12H)
BALB/c Mouse IgG1 MoAb
Cross-reacts with rat osteocalcin

Does not react with human and bovine osteocalcin

More Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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