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  • Bone specific alkaline phosphatase
  • Collagen type 2
  • Dentin matrix protein 1
  • Osteocalcin (bovine)
  • Osteocalcin (human)
  • Osteocalcin (mouse)
  • Osteocalcin (pig)
  • Osteocalcin (rat)
  • Osteocalcin carboxylated Gla-OC
  • Osteocalcin undercarboxylated Glu-OC
  • Osteonectin
  • Procollagen type I C-peptide
  • Tartrate-resistant acid phosphatase (TRACP)
Home › Products › Antibodies and ELISA › Primary antibodies and ELISAs by research area › Bone research › Osteocalcin (human)

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Human osteocalcin detection

Osteocalcin, also known as bone gamma-carboxyglutamate protein, is a vitamin K- and D-dependent, calcium-binding, non-collagenous protein. It has a molecular weight of approximately 5.9 kDa and contains 2–3 gamma-carboxyglutamate residues (Gla). Osteocalcin is produced only by osteoblasts and their dental counterpart, odontoblasts, and is an indicator of bone metabolism. The carboxylated form of osteocalcin is an indicator of bone formation, while its undercarboxylated counterpart is involved in glucose metabolism. Osteocalcin also acts as a hormone that regulates glucose and fat deposition as well as male fertility.

Osteocalcin, also known as bone gamma-carboxyglutamate protein, is a vitamin K- and D-dependent, calcium-binding, non-collagenous protein. It has a molecular weight of approximately 5.9 kDa and contains 2–3 gamma-carboxyglutamate residues (Gla). Osteocalcin is produced only by osteoblasts and their dental counterpart, odontoblasts, and is an indicator of bone metabolism. The carboxylated form of osteocalcin is an indicator of bone formation, while its undercarboxylated counterpart is involved in glucose metabolism. Osteocalcin also acts as a hormone that regulates glucose and fat deposition as well as male fertility.

Alternate names: bone gamma-carboxyglutamic acid-containing protein, BGLAP.

ELISA kits for osteocalcin detection

The Human Gla-Osteocalcin High Sensitive EIA Kit is designed to specifically differentiate human from bovine osteocalcin using a human osteocalcin-specific coated plate. This coated plate contains a monoclonal antibody that distinguishes human from bovine osteocalcin via their amino acid differences at positions 3 and 4 in the N-terminus. Using a human antigen-specific monoclonal antibody for capture greatly improves experimental linearity with human blood samples.

The specificity of this human osteocalcin ELISA also enables direct assay of human osteocalcin in osteoclast culture supernatant, differentiated bone marrow osteoblasts, and mesenchymal stem cells cultured in medium containing FBS. The detection antibody in this kit specifically recognizes the osteogenic marker Gla at position 17, allowing direct measurement of Gla-type (active type) osteocalcin in bone (primarily hydroxyapatite) matrix.

Antibodies for osteocalcin detection

Monoclonal Anti-Human Undercarboxylated Osteocalcin (Clone GluOC4-5; Cat. # M171) was raised against human osteocalcin peptide and specifically recognizes human osteocalcin with decarboxylated glutamic acid residues. This antibody does not recognize Gla-type osteocalcin. Monoclonal Anti-Human Osteocalcin (Clone 5-12H; Cat. # M184) was obtained by fusing mouse myeloma cell-line P3U1 with C57BL6 mouse spleen cells following immunization with keyhole limpet hemocyanin (KLH) conjugated to human osteocalcin peptide (amino acid residues 1–6). This monoclonal antibody was harvested from severe combined immunodeficiency (SCID) mouse ascitic fluid, and is useful for detection of full-length human osteocalcin.

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Cat. # Product Size Price License Quantity Details
MK128 Human Gla-Osteocalcin High Sensitive EIA Kit 96 Rxns USD $1002.00

This kit is designed to precisely differentiate bovine and human osteocalcins, using a capture antibody-coated plate with a human osteocalcin-specific monoclonal antibody that recognizes the distinct difference in the amino acids at positions 3 and 4 from the N-terminus. This makes possible differential assays of human and bovine osteocalcins. Use of human antigen-specific antibody as a capture monoclonal antibody provides improved linearity when assaying human blood samples. This specificity also enables direct assay of human osteocalcin in the culture supernatant of osteoblasts or differentiated osteoblasts from bone marrow or mesenchymal stem cells cultured in a bovine serum-containing medium, which has been difficult to achieve using the conventional Gla-OC EIA Kit (Cat. #MK111).

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Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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MK128: Human Gla-Osteocalcin High Sensitive EIA Kit

MK128: Human Gla-Osteocalcin High Sensitive EIA Kit
M184 Monoclonal Anti-Human Osteocalcin (Clone 5-12H) 0.1 mg USD $401.00

This monoclonal antibody was obtained by fusing the mouse myeloma cell-line P3U1 with spleen cells of C57BL6 mouse after immunization with KLH-conjugated peptide from human osteocalcin sequence (amino acids 1-6 YLYQWL). The monoclonal antibody was harvested from scid mouse ascitic fluid.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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M171 Monoclonal Anti-Human Undercarboxylated Osteocalcin (Clone GluOC4-5) 0.1 mg USD $401.00

This clone was raised against human osteocalcin peptide. It specifically recognizes human osteocalcin with decarboxylated glutamic acid residues, and does not recognize Gla-type osteocalcin.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Overview

Antigen/peptide Cat. # Application Species (Clone) and source Cross‑reactivity
Osteocalcin with Gamma-carboxyglutamate at position 17 (active type) MK128 ELISA Human Capture:
(n/a)
MoAb

Detect: HRP
(n/a)
MoAb
Does not cross‑react with bovine osteocalcin
Osteocalcin
(14-30) conjugated to KLH
M171 IHC, WB Human (GluOC4-5)
BALB/c Mouse IgG1 MoAb
Epitope: human osteocalcin with decarboxylated Glu-21 and Glu-24

Cross-reacts with bovine, rabbit, rat, dog, guinea pig, porcine, sheep, and goat osteocalcin with decarboxylated Glu-21 and Glu-24

Does not react with Gla‑type osteocalcin
Osteocalcin
(1-6) conjugated to KLH
M184 IHC, WB Human (5-12H)
C57BL6 Mouse IgG2b MoAb
Epitope: human osteocalcin at residues 1-6

Does not react with bovine, rat, guinea pig, porcine, horse, pigeon, or goose osteocalcin

More Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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