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Home › Products › Antibodies and ELISA › Primary antibodies and ELISAs by research area › Stem cell research antibodies › Neural progenitor cell antibodies

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Neural progenitor cell antibodies: Bf1, Crx, Emx1, Irx3, L7/Pcp2, Otp, Otx2, Rx, and Six3

Neural progenitor cells express characteristic protein biomarkers, and antibodies to these biomarkers allow analysis of cell differentiation during embryonic development and organogenesis. These antibodies are of particular use for studies in the field of regenerative medicine, may be used as neurogenesis differentiation markers with ES or iPS cells, and are suitable for immunohistochemistry on fixed tissue and Western blotting.

Neural progenitor cells express characteristic protein biomarkers, and antibodies to these biomarkers allow analysis of cell differentiation during embryonic development and organogenesis. These antibodies are of particular use for studies in the field of regenerative medicine, may be used as neurogenesis differentiation markers with ES or iPS cells, and are suitable for immunohistochemistry on fixed tissue and Western blotting.

L7 antibodies allow detection of Purkinje cells, which are the output neurons of the cerebellar cortex. Otp antibodies can be used to identify differentiating hypothalamic neuroendocrine cells. Antibodies to Emx1 allow detection of this cerebral cortical marker during early embryogenesis. Six3 antibodies have been used to study generation of rostral hypothalamic-like progenitors from mouse ES cell-derived neuroectodermal cells. Otx2 antibodies have been used to study differentiation of retinal progenitors in embryonic neural retina and induced ES cells in culture. The transcription factor detected by anti-Brain Factor 1 (Bf1) antibodies localizes to cerebral nerve precursor cells in a rostral region called the telencephalon. Rx antibodies detect a transcription factor critical for protein expression in retinal progenitor cells. Antibodies to Iroquois Homeobox Protein 3 (Irx3) can be used to study early development of the central nervous system and the caudal nerves. Detection of the photoreceptor-specific transcription factor Cone-rod homeobox protein (Crx) with anti-Crx antibodies enables study of the development and differentiation of retinal photoreceptor cells and the pineal gland.

These polyclonal antibodies were developed in the laboratory of Yoshiki Sasai, MD, PhD, Center for Developmental Biology (RIKEN).

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Cat. # Product Size License Quantity Details
M231 Anti-Human Crx, Polyclonal 0.2 mg USD $489.00

Antibody to neural progenitor cells; detects neurogenesis biomarkers. Use for embryogenesis and stem cell research. This is a rabbit polyclonal antibody raised against two peptide fragments of Human Crx (Cone-rod homeobox protein; a transcription factor that is specifically expressed in photoreceptors and pinealocytes and is important for development and functional homeostasis of cone cells and rod cells forming the retinal cells) conjugated with KLH.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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Alignment of peptide sequences from several Crx homologs

Alignment of peptide sequences from several Crx homologs
Alignment of peptide sequences from several Crx homologs.
M230 Anti-Mouse Irx3, Polyclonal 0.2 mg USD $495.00

Antibody to neural progenitor cells; detects neurogenesis biomarkers. Use for embryogenesis and stem cell research. This is a rabbit polyclonal antibody raised against C-terminal region peptide of mouse Irx3 (Iroquois related homeobox 3) [CSALE VEKKL LKTAF QPVPR RPQNH LDAAL VLSAL SSS] conjugated with KLH. Irx 3 is a transcription factor that is localized in the caudal nerve and expressed in the neural plate during the early development of the central nervous system.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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Alignment of the C-terminal amino acid sequences of several Irx3 homologs

Alignment of the C-terminal amino acid sequences of several Irx3 homologs
Alignment of the C-terminal amino acid sequences of several Irx3 homologs.

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Immunohistochemical Staining with Anti-Mouse Irx3, Polyclonal (Cat

Immunohistochemical Staining with Anti-Mouse Irx3, Polyclonal (Cat
Immunohistochemical Staining with Anti-Mouse Irx3, Polyclonal (Cat. No. M230) and DAPI. Tissue: Embryonic mouse, day 10 (E10). Fixation: 4% paraformaldehyde, 6 h. Permeabilization: 0.3% Triton X-100 in PBS. Blocking: 10% normal Donkey serum in PBS. Blue, DAPI stain; red, anti-Irx3 antibody.
M229 Anti-Human/Mouse Rx, Polyclonal (Guinea Pig) 0.2 mg USD $489.00

Antibody to neural progenitor cells; detects neurogenesis biomarkers. Use for embryogenesis and stem cell research. This is a guinea pig polyclonal antibody raised against three peptide fragments of Rx (Retinal homeobox protein; a homeobox transcription factor that functions in eye development and is expressed early in the eye primordi,) conjugated with KLH.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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Alignment of peptide sequences from several Rx homologs

Alignment of peptide sequences from several Rx homologs
Alignment of peptide sequences from several Rx homologs.

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Immunohistochemical Staining with Anti-Human/Mouse Rx, Polyclonal (Guinea Pig) (Cat

Immunohistochemical Staining with Anti-Human/Mouse Rx, Polyclonal (Guinea Pig) (Cat
Immunohistochemical Staining with Anti-Human/Mouse Rx, Polyclonal (Guinea Pig) (Cat. No. M229) and DAPI. Tissue: Mouse head, sagittal section. Fixation: 4% paraformaldehyde. Permeabilization: 0.3% Triton X-100 in PBS. Blue, DAPI stain; red, anti-Rx antibody.
M228 Anti-Mouse Rx, Polyclonal 0.2 mg USD $489.00

Antibody to neural progenitor cells; detects neurogenesis biomarkers. Use for embryogenesis and stem cell research. This is a rabbit polyclonal antibody raised against three peptide fragments of Rx (Retinal homeobox protein; a homeobox transcription factor that functions in eye development and is expressed early in the eye primordi,) conjugated with KLH.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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Immunohistochemical Staining with Anti-Mouse Rx, Polyclonal (Cat

Immunohistochemical Staining with Anti-Mouse Rx, Polyclonal (Cat
Immunohistochemical Staining with Anti-Mouse Rx, Polyclonal (Cat. No. M228) and DAPI. Tissue: Mouse head, sagittal section. Fixation: 4% paraformaldehyde. Permeabilization: 0.3% Triton X-100 in PBS. Blue, DAPI stain; red, anti-Rx antibody.

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Alignment of peptide sequences from several Rx homologs

Alignment of peptide sequences from several Rx homologs
Alignment of peptide sequences from several Rx homologs.
M227 Anti-Human/Mouse Bf1, Polyclonal 0.2 mg USD $489.00

Antibody to neural progenitor cells; detects neurogenesis biomarkers. Use for embryogenesis and stem cell research. This is a rabbit polyclonal antibody raised against C-terminal region peptide of human/mouse Brain factor 1 (Transcription Factor BF-1/forkhead box protein G1/FOXG1B) [SLPSF TTGLS GGLSD YFTHQ NQGSS SNPLI H] conjugated with bovine thyroglobulin. Brain factor 1 is a transcription factor that is localized in the neural progenitor cells for the cerebrum. It is responsible for the development of the telencephalon.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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Alignment of the C-terminal amino acid sequences of several Bf1 homologs

Alignment of the C-terminal amino acid sequences of several Bf1 homologs
Alignment of the C-terminal amino acid sequences of several Bf1 homologs.

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Immunohistochemical Staining with Anti-Human/Mouse Bf1, Polyclonal (Cat

Immunohistochemical Staining with Anti-Human/Mouse Bf1, Polyclonal (Cat
Immunohistochemical Staining with Anti-Human/Mouse Bf1, Polyclonal (Cat. No. M227) and DAPI. Tissue: Embryonic mouse, day 11 (E11). Fixation: 4% paraformaldehyde. Permeabilization: 0.3% Triton X-100 in PBS. Blue, DAPI stain; red, anti-Bf1 antibody.
M202 Polyclonal Antibody to Human L7/Pcp2 0.2 mg USD $489.00

Antibody to neural progenitor cells; detects neurogenesis biomarkers. Use for embryogenesis and stem cell research. This is a rabbit polyclonal antibody raised against C-terminal region peptide [TALGF RRNSS PQPPT QAP] of human L7/Pcp2 (a marker of the purkinje progenitor cells in brain and nervous system) conjugated with KLH.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components
M198 Polyclonal Antibody to Mouse Otx2 100 uL USD $489.00

Antibody to neural progenitor cells; detects neurogenesis biomarkers. Use for embryogenesis and stem cell research. This is a rabbit polyclonal antibody raised against C-terminal region peptide [CGSYL TPMHH QLPGP GATLS PMGTN] of mouse Otx2 [Orthdenticle homeobox 2; a marker of the most upstream transcription factor that controls the differentiation of the forebrain and the retinal photoreceptor cells (cone cells and rod cells) in embryonic development] conjugated with KLH.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

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Immunohistochemical Staining with Polyclonal Antibody to Mouse Otx2 (Cat

Immunohistochemical Staining with Polyclonal Antibody to Mouse Otx2 (Cat
Immunohistochemical Staining with Polyclonal Antibody to Mouse Otx2 (Cat. No. M198) and DAPI. Tissue: Embryonic mouse, day 10 (E10), forebrain to midbrain. Fixation: 4% paraformaldehyde, 6 h. Permeabilization: 0.3% Triton X-100 in PBS. Blocking: 2% skim milk in PBS. Blue, DAPI stain; red, anti-Otx2 antibody.

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Alignment of the C-terminal amino acid sequences of several Otx2 homologs

Alignment of the C-terminal amino acid sequences of several Otx2 homologs
Alignment of the C-terminal amino acid sequences of several Otx2 homologs.
M196 Polyclonal Antibody to Mouse Emx1 100 uL USD $489.00

Antibody to neural progenitor cells; detects neurogenesis biomarkers. Use for embryogenesis and stem cell research. This is a guinea pig polyclonal antibody raised against C-terminal region peptide [ESEQK KKGSH HINRW RIATK QANGE DIDVT SND] of mouse Emx 1 (Empty spiracles homeobox 1; marker of embryonic development in cerebral cortex neurons) conjugated with KLH.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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Amino acid alignment of the C-terminal regions of several Emx1 homologs

Amino acid alignment of the C-terminal regions of several Emx1 homologs
Amino acid alignment of the C-terminal regions of several Emx1 homologs.

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Immunohistochemical Staining with Polyclonal Antibody to Mouse Emx1 (Cat

Immunohistochemical Staining with Polyclonal Antibody to Mouse Emx1 (Cat
Immunohistochemical Staining with Polyclonal Antibody to Mouse Emx1 (Cat. No. M196). Tissue: Embryonic mouse, 10.5 days (E10.5), sagittal section of telencephalon, dorsal region located at top. Fixation: 4% paraformaldehyde. Permeabilization: 0.3% Triton X-100 in PBS. Blocking: 2% skim milk in PBS.
M195 Polyclonal Antibody to Mouse Otp 100 uL USD $489.00

Antibody to neural progenitor cells; detects neurogenesis biomarkers. Use for embryogenesis and stem cell research. Otp is a guinea pig polyclonal antibody raised against C-terminal region peptide [LRRKA LEHTV SMSFT] of mouse Otp (homeobox protein Orthopedia; a marker of the hindbrain and hypothalamic neurons during embryonic development ) conjugated with KLH.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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Alignment of the C-terminal amino acid sequences of several homologs of Otp

Alignment of the C-terminal amino acid sequences of several homologs of Otp
Alignment of the C-terminal amino acid sequences of several homologs of Otp.

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Immunohistochemical Staining with Polyclonal Antibody to Mouse Otp (Cat

Immunohistochemical Staining with Polyclonal Antibody to Mouse Otp (Cat
Immunohistochemical Staining with Polyclonal Antibody to Mouse Otp (Cat. No. M195) and DAPI. Tissue: Embryonic mouse, 13.5 days (E13.5), hypothalamus, hindbrain, rostral. Fixation: 4% paraformaldehyde, 6 h. Permeabilization: 0.3% Triton X-100 in PBS. Blocking: 2% skim milk in PBS. .
M194 Polyclonal Antibody to Mouse L7/Pcp2 100 uL USD $489.00

Antibody to neural progenitor cells; detects neurogenesis biomarkers. Use for embryogenesis and stem cell research. L7/Pcp2 is a rabbit polyclonal antibody raised against C-terminal region peptide [AALSF RRNSS PQPQT QAP] of mouse L7/Pcp2 (a marker of the purkinje progenitor cells in brain and nervous system) conjugated with KLH.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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Alignment of C-terminal amino acid sequence of mouse, human, bovine, rat, and pig L7/Pcp2 homologs

Alignment of C-terminal amino acid sequence of mouse,  human, bovine, rat, and pig L7/Pcp2 homologs
Alignment of C-terminal amino acid sequence of mouse, human, bovine, rat, and pig L7/Pcp2 homologs.

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Immunohistochemical staining with Polyclonal Antibody to Mouse L7/Pcp2Postnatal (Cat

Immunohistochemical staining with Polyclonal Antibody to Mouse L7/Pcp2Postnatal (Cat
Immunohistochemical staining with Polyclonal Antibody to Mouse L7/Pcp2Postnatal (Cat. No. M194). Tissue: 1-day-old mouse cerebellum, sagittal section. Fixation: 4% paraformaldehyde. Permeabilization: 0.3% Triton X-100 in PBS. Blocking: 2% skim milk in PBS.

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Immunohistochemical detection of neural progenitor cells in mouse cerebellum

Immunohistochemical detection of neural progenitor cells in mouse cerebellum

Immunohistochemical detection of neural progenitor cells in mouse cerebellum. A section of formalin-fixed paraffin-embedded mouse cerebellum tissue was stained with an anti-L7/Pcp2 antibody (Cat. # M194). Takara POD Conjugate Anti Rabbit, For Mouse Tissue (Cat. # MK202) was used in place of a traditional secondary antibody, and Takara DAB Substrate (Cat. # MK210) was used for detection. The image shown is 100X magnification.

Overview

Neural progenitor cells: antibody specifications

  Cell type detected Antigen Species Specificity Cat. #
Bf1

Cerebral neural progenitor cells in the telencephalon

C‑terminal peptide of human/mouse Bf1 conjugated with bovine thyroglobulin

Human
Mouse

C‑terminal region of human and mouse Bf1

M227

Crx

Retinal photoreceptor cells (cone and rod cells) during embryonic development

KLH‑conjugated peptide fragments of human Crx

Human

Human Crx

M231

Emx1

Cerebral cortex neurons during embryonic development

KLH‑conjugated peptide of mouse Emx1

Mouse

C‑terminal region of mouse Emx1

M196

Irx3

Neural plate progenitor cells involved in caudal nerve development

KLH‑conjugated peptide from the C‑terminal region of mouse Irx3

Mouse

Mouse Irx3

M230

L7/Pcp2

Purkinje progenitor cells

KLH‑conjugated peptide from the C‑terminal region of L7/Pcp2

Mouse

C‑terminal region of mouse L7/Pcp2

M194

Human

C‑terminal region of mouse L7/Pcp2; Reacts with human & mouse L7/Pcp2

M202

Otp

Hindbrain and hypothalamic neurons during embryonic development

KLH‑conjugated peptide from the C‑terminal region of mouse Otp

Mouse

C‑terminal region of mouse Otp

M195

Otx2

Retinal photoreceptor cells during embryonic development

KLH‑conjugated peptide from the C‑terminal region of Otx2

Mouse

C‑terminal region of mouse Otx2

M198

Human

C‑terminal region of human Otx2

M199

Rx

Retinal progenitor cells

KLH‑conjugated peptide fragments of Rx

Mouse

Mouse Rx

M228

Human
Mouse

Human & mouse Rx

M229

Six3

Rostral brain progenitors; forebrain and retina, early embryonic and CNS development

KLH‑conjugated peptide from the C‑terminal region of mouse Six3

Mouse

C‑terminal region of mouse Six3

M197

More Information

Applications

  •     Immunohistochemical staining on fixed tissue

  •     Western blotting under reducing conditions (Cat. # M196, M197, M228, M229, M230, M231)

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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What does it take to generate good science? Careful planning, dedicated researchers, and the right tools. At Takara Bio, we thoughtfully develop best-in-class products to tackle your most challenging research problems, and have an expert team of technical support professionals to help you along the way, all at superior value.

Explore what makes good science possible

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Clontech, TaKaRa, cellartis

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