GMP-140 (P-selectin) EIA Kit
The GMP-140 (P-selectin) EIA Kit is a 96-well in vitro enzyme immunoassay kit for quantitative determination of human GMP-140 in plasma, serum, cultured cell extracts, cell culture supernatants and other biological fluids. It is a solid phase sandwich EIA that utilizes two mouse monoclonal GMP-140 antibodies (one of which is coated on the plate, and the other is POD-labeled) for detection of human GMP-140 using a two-step incubation method.
The GMP-140 (P-selectin) EIA Kit is a 96-well in vitro enzyme immunoassay kit for quantitative determination of human GMP-140 in plasma, serum, cultured cell extracts, cell culture supernatants and other biological fluids. It is a solid phase sandwich EIA that utilizes two mouse monoclonal GMP-140 antibodies (one of which is coated on the plate, and the other is POD-labeled) for detection of human GMP-140 using a two-step incubation method.
In the first step, samples are incubated in the antibody-coated microtiter plate. During the second step, the plate is washed and incubated with the POD-labeled GMP-140 antibody. A substrate is added, and the reaction between POD and the substrate (H2O2, TMBZ) results in a color development. The amount of sample GMP-140 is determined by measuring absorbance using an EIA plate reader. Accurate GMP-140 sample concentrations can be determined by comparing their specific absorbances with the absorbance obtained for the Standard plotted on a standard curve.
Granule Membrane Protein 140 (GMP-140, P-selectin, CD62 or PADGEM) is a 140 kDa integral membrane adhesion glycoprotein categorized functionally as a member of the LEC-CAM (lectin-epidermal growth factor-complement binding cell adhesion molecules) family or the selectin family. In non-activated conditions, GMP-140 is present in the alpha-granules of platelets or in endothelial cell Weibel Palade bodies. However, when activated by stimulators, such as thrombin or by inflammatory or hemostatic mediators during intravascular inflammation, GMP-140 is rapidly secreted to the plasma membrane to promote adhesion of cells to vascular endothelia or to neutrophils and monocytes at the site of tissue injury. Previous studies suggested that GMP-140 is indeed a useful marker of platelet and endothelium activation in experimental inflammation models, and potentially in clinical situations. Recent data also suggest that free GMP-140 molecules present in the circulation may serve to maintain the non-adhesiveness of neutrophils and prevent the development of inflammatory responses.
Overview
- Sandwich ELISA: quantitative determination of human GMP-140 in plasma, serum, cultured cell extracts, cell culture supernatants and other biological fluids
- Sensitive: detect as little as 20 ng/ml
More Information
Applications
- Quantitative Measurement of Human GMP-140
Components
| Antibody-coated microtiter plate | 1 plate (8 well x 12 strips) |
| Antibody-POD conjugate (lyophilized) | 1 vial (for 1 x 11 ml) |
| Standard (lyophilized) | 1 vial (for 1 x 1 ml) |
| Sample diluent | 2 vials (2 x 11 ml) |
| Substrate solution | 1 vial (1 x 12 ml) |
Storage
2–8°C
Performance characteristics
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Range of assay: 20–640 ng/ml
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Assay sensitivity: 20 ng/ml
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Assay duration: 3.5 hours
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Specificity: Human GMP-140. No reactivity with platelet Gp Ib, platelet Gp IIb, platelet Gp IIIa, von Willebrand factor, or platelet thrombospondin.
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Species cross-reactivity: No reactivity with mouse GMP-140. Applications using other sources have not been tested.
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Test specimen type: Plasma, culture supernatants, cell extracts, or other biological fluids
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Sample volume per well: 100 µl
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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