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Home › Products › Antibodies and ELISA › Primary antibodies and ELISAs by research area › Signal transduction › GMP-140

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GMP-140 (P-selectin) EIA Kit

The GMP-140 (P-selectin) EIA Kit is a 96-well in vitro enzyme immunoassay kit for quantitative determination of human GMP-140 in plasma, serum, cultured cell extracts, cell culture supernatants and other biological fluids. It is a solid phase sandwich EIA that utilizes two mouse monoclonal GMP-140 antibodies (one of which is coated on the plate, and the other is POD-labeled) for detection of human GMP-140 using a two-step incubation method.

The GMP-140 (P-selectin) EIA Kit is a 96-well in vitro enzyme immunoassay kit for quantitative determination of human GMP-140 in plasma, serum, cultured cell extracts, cell culture supernatants and other biological fluids. It is a solid phase sandwich EIA that utilizes two mouse monoclonal GMP-140 antibodies (one of which is coated on the plate, and the other is POD-labeled) for detection of human GMP-140 using a two-step incubation method.

In the first step, samples are incubated in the antibody-coated microtiter plate. During the second step, the plate is washed and incubated with the POD-labeled GMP-140 antibody. A substrate is added, and the reaction between POD and the substrate (H2O2, TMBZ) results in a color development. The amount of sample GMP-140 is determined by measuring absorbance using an EIA plate reader. Accurate GMP-140 sample concentrations can be determined by comparing their specific absorbances with the absorbance obtained for the Standard plotted on a standard curve.

Granule Membrane Protein 140 (GMP-140, P-selectin, CD62 or PADGEM) is a 140 kDa integral membrane adhesion glycoprotein categorized functionally as a member of the LEC-CAM (lectin-epidermal growth factor-complement binding cell adhesion molecules) family or the selectin family. In non-activated conditions, GMP-140 is present in the alpha-granules of platelets or in endothelial cell Weibel Palade bodies. However, when activated by stimulators, such as thrombin or by inflammatory or hemostatic mediators during intravascular inflammation, GMP-140 is rapidly secreted to the plasma membrane to promote adhesion of cells to vascular endothelia or to neutrophils and monocytes at the site of tissue injury. Previous studies suggested that GMP-140 is indeed a useful marker of platelet and endothelium activation in experimental inflammation models, and potentially in clinical situations. Recent data also suggest that free GMP-140 molecules present in the circulation may serve to maintain the non-adhesiveness of neutrophils and prevent the development of inflammatory responses.

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Cat. # Product Size License Quantity Details
M063 Monoclonal Anti-Human Platelet GMP-140 (P-selectin/CD62) (Clone PL7-6) 0.1 mg USD $545.00

This clone was raised against activated human platelet GMP-140.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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M063: Anti-Human Platelet GMP-140 (CD62), Monoclonal (Clone PL7-6)

M063: Anti-Human Platelet GMP-140 (CD62), Monoclonal (Clone PL7-6)
M062 Monoclonal Anti-Human Platelet GMP-140 (P-selectin/CD62) (Clone WGA-1) 0.1 mg USD $545.00

This clone was raised against activated human platelet GMP-140.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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M062: Anti-Human Platelet GMP-140 (CD62), Monoclonal (Clone WGA-1)

M062: Anti-Human Platelet GMP-140 (CD62), Monoclonal (Clone WGA-1)

Overview

  • Sandwich ELISA: quantitative determination of human GMP-140 in plasma, serum, cultured cell extracts, cell culture supernatants and other biological fluids
  • Sensitive: detect as little as 20 ng/ml

More Information

Applications

  • Quantitative Measurement of Human GMP-140

Components

Antibody-coated microtiter plate 1 plate (8 well x 12 strips)
Antibody-POD conjugate (lyophilized) 1 vial (for 1 x 11 ml)
Standard (lyophilized) 1 vial (for 1 x 1 ml)
Sample diluent 2 vials (2 x 11 ml)
Substrate solution 1 vial (1 x 12 ml)

 

Performance characteristics

  • Range of assay: 20–640 ng/ml

  • Assay sensitivity: 20 ng/ml

  • Assay duration: 3.5 hours

  • Specificity: Human GMP-140. No reactivity with platelet Gp Ib, platelet Gp IIb, platelet Gp IIIa, von Willebrand factor, or platelet thrombospondin.

  • Species cross-reactivity: No reactivity with mouse GMP-140. Applications using other sources have not been tested.

  • Test specimen type: Plasma, culture supernatants, cell extracts, or other biological fluids

  • Sample volume per well: 100 µl

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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