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NucleoSpin Microbial DNA product page NucleoSpin Microbial DNA product page
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Product overview

Genomic DNA from hard-to-lyse bacteria, yeast, and other fungi

The NucleoSpin Microbial DNA kit is designed for efficient isolation of genomic DNA from challenging microbial samples that are typically hard to lyse. DNA can be isolated from a wide variety of microorganisms such as gram-negative and gram-positive bacteria as well as yeast, e.g., Escherichia coli, Bacillus subtilis, Corynebacterium glutamicum, and Saccharomyces cerevisiae.

The kit utilizes NucleoSpin Bead Tubes Type B (glass beads; included in the kit) in combination with Liquid Proteinase K, and a common disruption device e.g., swing mill, in order to lyse different sample materials with one procedure. The procedure is fast, simple and reliably high DNA yields from a wide range of microbial samples can be obtained. Additional NucleoSpin Bead Tubes can be ordered separately.

  • Suitable for a wide variety of starting materials
  • NucleoSpin Bead Tubes are included for efficient lysis—compatible with many disruption devices
  • Fast and easy procedure—Liquid Proteinase K included; no additional enzyme required
At-a-glance Homogenization Application data Microorganisms tested Protocol

At-a-glance  

Technology Silica membrane technology
Format Mini spin columns
Sample material <40 mg wet weight cell pellet
Fragment size 200 bp–approx. 50 kbp
Typical yield Depends on sample type and disruption. Approx. 5–25 µg (30 mg wet weight cell pellet)
A260/A280 1.6–2.0
Elution volume 100–200 µl
Preparation time 35 min/prep
Binding capacity 60 µg

Homogenization  

NucleoSpin Bead Tube Type B

NucleoSpin Bead Tubes Type B. The NucleoSpin Microbial DNA kit utilizes NucleoSpin Bead Tubes Type B (40–400 µm glass beads; included in the kit) in combination with Liquid Proteinase K, and a MN Bead Tube Holder (Cat. No. 740469, sold separately) on a Vortex-Genie 2 or with a mixer mill (Retsch) in order to lyse different sample materials with one procedure.

Application data  

Efficient DNA recovery from different microorganisms.

Figure 1. Efficient DNA recovery from different microorganisms. DNA was isolated with the NucleoSpin Microbial DNA kit and NucleoSpin Bead Tubes Type B (included in the kit) or NucleoSpin Bead Tubes Type C. 100 ng DNA per prep was analyzed by agarose gel electrophoresis showing high molecular DNA without RNA contamination or DNA degradation.

  1. Escherichia coli, NucleoSpin Bead Tubes Type B 
  2. Vibrio fischerii, NucleoSpin Bead Tubes Type B 
  3. Bacillus subtilis, NucleoSpin Bead Tubes Type B 
  4. Corynebacterium glutamicum, NucleoSpin Bead Tubes Type B 
  5. Saccharomyces cerevisiae, NucleoSpin Bead Tubes Type C

Comparison of preparation times

Figure 2. Comparison of preparation times. The prep time of NucleoSpin Microbial DNA kit was compared with competitor products by processing Bacillus subtilis, Escherichia coli, and Saccharomyces cerevisiae. The NucleoSpin Microbial DNA kit shows shorter prep times in all three cases.

Microorganisms tested  

DNA has been successfully isolated from the microorganisms listed below.

Microorganisms Tested by
Acinetobacter Customer
Aspergillus spec. MN
Bacillus subtilis Customer
Clostridium ljungdahlii Customer
Corynebacterium glutamicum MN
Escherichia coli MN
Eurotium spec. Customer
Klebsiella pneumoniae Customer
Microbacterium spec. Customer
Pichia pastoris Customer
Pseudomonas aeruginosa Customer
Rhizopus spec. MN
Saccharomyces cerevisiae MN
Staphylococcus pneumoniae Customer
Streptococcus pneumoniae
Customer
Trametes spec. Customer
Vibrio fischerii MN

Protocol  

Workflow

Related Products

Cat. # Product Size Price License Quantity Details
740469 MN Bead Tube Holder Each USD $34.70

Blue rubber foam adapter for processing NucleoSpin Bead Tubes with a vortex apparatus (e.g., Vortex Genie 2). NucleoSpin Bead Tubes are 2-ml screw cap plastic tubes containing different types of beads (glass, ceramic, steel, or corundum). They are designed for the homogenization of hard-to-lyse biological samples (e.g., soil, stool, insect, and some microbial samples) prior to purification of nucleic acids.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740469: MN Bead Tube Holder

740469: MN Bead Tube Holder
740812.50 NucleoSpin® Bead Tubes Type B 50 Preps USD $78.60

Pack of fifty 2-ml tubes containing glass beads for sample homogenization. Use in combination with our MN Bead Tube Holder (Cat. # 740469) and NucleoSpin Microbial DNA miniprep kits (e.g., Cat. # 740235.50) for isolation of total DNA from bacterial samples.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740812.50: NucleoSpin Bead Tubes Type B

740812.50: NucleoSpin Bead Tubes Type B

Required Products

Cat. # Product Size Price License Quantity Details
740235.50 NucleoSpin® Microbial DNA 50 Preps USD $198.00

Miniprep kit (50 preps) designed for rapid purification of highly pure genomic DNA from microorganisms (gram-negative and gram-positive bacteria, yeast, and fungi). The NucleoSpin Microbial DNA kit utilizes NucleoSpin Bead Tubes Type B (glass beads; included in the kit) in combination with Liquid Proteinase K and a common disruption device, e.g., a swing mill, to lyse different starting materials using a single procedure. The procedure is fast, simple, and provides reliably high DNA yields from a wide range of microbial samples. NucleoSpin Bead Tubes Type B can also be ordered separately.

Documents You May Also Like Image Data

Back

740235.50: NucleoSpin Microbial DNA

740235.50: NucleoSpin Microbial DNA
740235.50 NucleoSpin® Microbial DNA 50 Preps USD $198.00

Miniprep kit (50 preps) designed for rapid purification of highly pure genomic DNA from microorganisms (gram-negative and gram-positive bacteria, yeast, and fungi). The NucleoSpin Microbial DNA kit utilizes NucleoSpin Bead Tubes Type B (glass beads; included in the kit) in combination with Liquid Proteinase K and a common disruption device, e.g., a swing mill, to lyse different starting materials using a single procedure. The procedure is fast, simple, and provides reliably high DNA yields from a wide range of microbial samples. NucleoSpin Bead Tubes Type B can also be ordered separately.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

Back

740235.50: NucleoSpin Microbial DNA

740235.50: NucleoSpin Microbial DNA
740235.10 NucleoSpin® Microbial DNA 10 Preps USD $47.20

Mini prep kit (10 preps) designed for rapid purification of highly pure genomic DNA from microorganisms (gram-negative and gram-positive bacteria, yeast, and fungi). The NucleoSpin Microbial DNA kit utilizes NucleoSpin Bead Tubes Type B (glass beads; included in the kit) in combination with Liquid Proteinase K, and a common disruption device e.g., swing mill, in order to lyse different sample materials with one procedure. The procedure is fast, simple and reliably high DNA yields from a wide range of microbial samples can be obtained. NucleoSpin Bead Tubes Type B can also be ordered separately.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

Back

740235.10: NucleoSpin Microbial DNA

740235.10: NucleoSpin Microbial DNA

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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