|Technology||Silica membrane technology|
|Format||Mini spin columns|
|Sample material||<40 mg wet weight cell pellet|
|Fragment size||200 bp–approx. 50 kbp|
|Typical yield||Depends on sample type and disruption. Approx. 5–25 µg (30 mg wet weight cell pellet)|
|Elution volume||100–200 µl|
|Preparation time||35 min/prep|
|Binding capacity||60 µg|
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- Plasmid purification
- Genomic DNA purification
- DNA/RNA cleanup and extraction
- RNA purification
- Parallel DNA, RNA & protein
- Hard-to-lyse samples
Genomic DNA from hard-to-lyse bacteria, yeast, and other fungi
The NucleoSpin Microbial DNA kit is designed for efficient isolation of genomic DNA from challenging microbial samples that are typically hard to lyse. DNA can be isolated from a wide variety of microorganisms such as gram-negative and gram-positive bacteria as well as yeast, e.g., Escherichia coli, Bacillus subtilis, Corynebacterium glutamicum, and Saccharomyces cerevisiae.
The kit utilizes NucleoSpin Bead Tubes Type B (glass beads; included in the kit) in combination with Liquid Proteinase K, and a common disruption device e.g., swing mill, in order to lyse different sample materials with one procedure. The procedure is fast, simple and reliably high DNA yields from a wide range of microbial samples can be obtained. Additional NucleoSpin Bead Tubes can be ordered separately.
- Suitable for a wide variety of starting materials
- NucleoSpin Bead Tubes are included for efficient lysis—compatible with many disruption devices
- Fast and easy procedure—Liquid Proteinase K included; no additional enzyme required
DNA has been successfully isolated from the microorganisms listed below.
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