|Format||Mini spin columns|
|Sample material||1–25 mg tissue; 102–107 cells|
|Fragment size||200 bp to >30 kbp|
|Typial yield||20–35 μg|
|Binding capacity||60 μg|
|Typical Ratio A260/A280||1.7–1.9|
|Elution volume||60–100 μl|
|Preparation time||~20 min/prep (excluding lysis)|
- Product finder
- Plasmid purification
- Genomic DNA purification
- DNA/RNA cleanup and extraction
- RNA purification
- Parallel DNA, RNA & protein
- Hard-to-lyse samples
- High DNA recovery and purity
- Reliable DNA purification for reproducible results
- Over 16 optimized support protocols for a wide variety of starting materials
- Also available for QIAcube (contact technical support)
NucleoSpin Tissue kits are designed for the rapid purification of highly pure genomic DNA from tissue samples, mouse tails, bacteria, yeast, forensic samples (hair, dried blood spots, buccal swabs, cigarette filters, etc.), and clinical samples (stool, urine).
At a glance
NucleoSpin Tissue shows proven reliability in DNA purification from different sample materials. The following table presents a selection of peer-reviewed publications citing NucleoSpin Tissue.
|Sample material||DNA application||Publication|
|Dried blood spots on newborn screening cards||DNA virus detection by PCR||C. S. Gibson et al., BMJ 332 (2006)|
|Buccal swabs||PCR of Y-chromosomal STR loci||H. Rodig et al., Int J Legal Med. 121 (2007)|
|Mice ear markings||PCR of Car9 and Car2 gene targets||P. Pan et al., J. Physiol. 571 (2006)|
|Cells (ciliate Oxytricha trifallax)||PCR||M. Nowacki et al., Nature 451 (2007)|
|FFPE (formaline-fixed paraffin-embedded) tissue||Determination of the methylation status of a promoter||R. Schneider-Stock et al., J. Clin. Oncol. 21 (2003)|
|Ants (ethanol preserved)||PCR, target: mitochondrial DNA, cytochrome oxidase||R. Savolainen and K. Vepsäläinen, PNAS 100 (2003)|
|Rat tails and embryonic tissue||Genotyping to distinguish between wild type and aralar-deficient animals||B. Pardo et al., J. Biol. Chem. 281 (2006)|
|Microdissected frozen and/or paraffin-embedded tissue||Mutation analysis using PCR and automated sequencing||V. Máximo et al., British Journal of Cancer 92 (2005)|
|Malignant melanoma||Array—CGH and mutation analysis||G. Jönsson et al., Oncogene 26 (2007)|
|Wasp leg, small pieces (1 mm long)||PCR, a 658-bp target, near the 5' terminus of the CO1 gene||M. A. Smith et al., PNAS 105 (2008)|
|Cells, parental and lentivirally transduced||PCR, Target: HSV-TkEGFP||R. Uch et al., Cancer Gene Therapy 10, 2003|
Data: aged samples
Data: clinical samples
This gel shows PCR results of DNA purified from urine, liquor, feces, mother's milk, and plasma with NucleoSpin Tissue. CMV can be detected in mother's milk as well as in several samples from the child (marked with arrows). The other samples do not carry the virus.
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