- Gene editing product finder
- Gene editing tools and information
- CRISPR/Cas9 knockouts
- CRISPR/Cas9 knockins
- Genome-wide screening
- Creating and screening for SNPs
- CRISPR/Cas9 delivery methods
- Cre recombinase
CRISPR/Cas9 delivery methods
For gene editing in mammalian cells we typically recommend delivery of a ribonuclear protein (RNP) complex consisting of Cas9 protein and a single guide RNA (sgRNA). RNPs remain in the cell for a short time and the dose is minimal, leading to lower toxicity and reduced editing at off-target sites compared to other methods.
RNPs can be delivered using either electroporation of recombinant Cas9 protein along with in vitro transcribed guide RNA or using cell-derived nanovesicles called gesicles. We also provide kits for delivery using lentivirus, AAV, and plasmid. See the links below for articles and tech notes related to delivery of CRISPR/Cas9 elements.
Delivery of RNPs using electroporation
Delivery of RNPs using gesicles
CRISPR-mediated editing using virus or plasmid
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2022 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.