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- CRISPR/Cas9 knockins
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- CRISPR/Cas9 delivery methods
- Cre recombinase
CRISPR/Cas9 delivery methods
For gene editing in mammalian cells we typically recommend delivery of a ribonuclear protein (RNP) complex consisting of Cas9 protein and a single guide RNA (sgRNA). RNPs remain in the cell for a short time and the dose is minimal, leading to lower toxicity and reduced editing at off-target sites compared to other methods.
RNPs can be delivered using either electroporation of recombinant Cas9 protein along with in vitro transcribed guide RNA or using cell-derived nanovesicles called gesicles. We also provide kits for delivery using lentivirus, AAV, and plasmid. See the links below for articles and tech notes related to delivery of CRISPR/Cas9 elements.
Delivery of RNPs using electroporation
Delivery of RNPs using gesicles
CRISPR-mediated editing using virus or plasmid
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