NucleoSpin RNA Plus XS—fast, efficient RNA isolation from the smallest samples

Rapidly and reliably purify total RNA from tiny samples, including single mammalian cells

The NucleoSpin RNA Plus XS kit is designed to purify RNA from very small amounts of a wide variety of cell and tissue types, as well as from single mammalian cells. This kit includes the NucleoSpin gDNA Removal Column XS, a spin column that quickly and effectively removes genomic DNA without the need for DNase digestion. See our NucleoSpin RNA Plus XS technical note for more information and data.

Cat. #	Product	Size	Price
740990.10	NucleoSpin® RNA Plus XS	10 Preps	USD $110.00
The NucleoSpin RNA Plus XS kit (10 preps) is optimized for efficient isolation of high-quality RNA from extra-small (XS) amounts of cells and tissue. The included NucleoSpin gDNA Removal Column XS provides enzyme-free DNA removal, saving 15 min in prep time compared to standard DNA digestion methods. NucleoSpin RNA Plus XS uses a novel lysis buffer chemistry that eliminates the need for reducing agents such as beta-mercaptoethanol or TCEP, avoiding unpleasant odors and health concerns. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back 740990.10: NucleoSpin RNA Plus XS Back Efficient removal of genomic DNA contamination with NucleoSpin RNA Plus XS Efficient removal of genomic DNA contamination with NucleoSpin RNA Plus XS. Total RNA was purified from the indicated amounts of HeLa cells using NucleoSpin RNA Plus XS or NucleoSpin RNA XS, respectively, by following the standard protocol for each kit (the former employs a gDNA removal column while the latter includes an on-column DNase digestion step). The amount of residual genomic DNA in the purified sample varied depending on the input amount, but for the input range of 10–10,000 cells, the NucleoSpin RNA Plus XS kit provided more efficient removal of genomic DNA than the NucleoSpin RNA XS kit. Back Efficient recovery of total RNA across varying input amounts with NucleoSpin RNA Plus XS Efficient recovery of total RNA across varying input amounts with NucleoSpin RNA Plus XS. Total RNA was purified from the indicated amounts of mouse liver tissue using NucleoSpin RNA Plus XS or kits from Competitor Q/Q+, I, and Z, respectively. Yields of total RNA obtained with NucleoSpin RNA Plus XS were greater than or comparable to yields obtained with each competitor kit for each of the input amounts processed.
740990.250	NucleoSpin® RNA Plus XS	250 Preps	USD $2060.00
The NucleoSpin RNA Plus XS kit (250 preps) is optimized for efficient isolation of high-quality RNA from extra-small (XS) amounts of cells and tissue. The included NucleoSpin gDNA Removal Column XS provides enzyme-free DNA removal, saving 15 min in prep time compared to standard DNA digestion methods. NucleoSpin RNA Plus XS uses a novel lysis buffer chemistry that eliminates the need for reducing agents such as beta-mercaptoethanol or TCEP, avoiding unpleasant odors and health concerns. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back 740990.250: NucleoSpin RNA Plus XS Back Efficient removal of genomic DNA contamination with NucleoSpin RNA Plus XS Efficient removal of genomic DNA contamination with NucleoSpin RNA Plus XS. Total RNA was purified from the indicated amounts of HeLa cells using NucleoSpin RNA Plus XS or NucleoSpin RNA XS, respectively, by following the standard protocol for each kit (the former employs a gDNA removal column while the latter includes an on-column DNase digestion step). The amount of residual genomic DNA in the purified sample varied depending on the input amount, but for the input range of 10–10,000 cells, the NucleoSpin RNA Plus XS kit provided more efficient removal of genomic DNA than the NucleoSpin RNA XS kit. Back Efficient recovery of total RNA across varying input amounts with NucleoSpin RNA Plus XS Efficient recovery of total RNA across varying input amounts with NucleoSpin RNA Plus XS. Total RNA was purified from the indicated amounts of mouse liver tissue using NucleoSpin RNA Plus XS or kits from Competitor Q/Q+, I, and Z, respectively. Yields of total RNA obtained with NucleoSpin RNA Plus XS were greater than or comparable to yields obtained with each competitor kit for each of the input amounts processed.
740990.50	NucleoSpin® RNA Plus XS	50 Preps	USD $466.00
The NucleoSpin RNA Plus XS kit (50 preps) is optimized for efficient isolation of high-quality RNA from extra-small (XS) amounts of cells and tissue. The included NucleoSpin gDNA Removal Column XS provides enzyme-free DNA removal, saving 15 min in prep time compared to standard DNA digestion methods. NucleoSpin RNA Plus XS uses a novel lysis buffer chemistry that eliminates the need for reducing agents such as beta-mercaptoethanol or TCEP, avoiding unpleasant odors and health concerns. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back Efficient removal of genomic DNA contamination with NucleoSpin RNA Plus XS Efficient removal of genomic DNA contamination with NucleoSpin RNA Plus XS. Total RNA was purified from the indicated amounts of HeLa cells using NucleoSpin RNA Plus XS or NucleoSpin RNA XS, respectively, by following the standard protocol for each kit (the former employs a gDNA removal column while the latter includes an on-column DNase digestion step). The amount of residual genomic DNA in the purified sample varied depending on the input amount, but for the input range of 10–10,000 cells, the NucleoSpin RNA Plus XS kit provided more efficient removal of genomic DNA than the NucleoSpin RNA XS kit. Back Efficient recovery of total RNA across varying input amounts with NucleoSpin RNA Plus XS Efficient recovery of total RNA across varying input amounts with NucleoSpin RNA Plus XS. Total RNA was purified from the indicated amounts of mouse liver tissue using NucleoSpin RNA Plus XS or kits from Competitor Q/Q+, I, and Z, respectively. Yields of total RNA obtained with NucleoSpin RNA Plus XS were greater than or comparable to yields obtained with each competitor kit for each of the input amounts processed. Back 740990.50: NucleoSpin RNA Plus XS

Overview

Developed for extra small samples and single cell analysis
gDNA removal column eliminates the need for time-consuming DNA digestion
Novel lysis buffer does not contain beta-mercaptoethanol or TCEP

More Information

Technology	Silica membrane
Format	Two columns: XS column for DNA removal XS column for RNA isolation
Starting material	Cultured cells (1–10⁵) Human/animal tissue (<5 mg)
Typical yield	HeLa cells (10⁵): 0.5–2 μg HeLa cells (10¹): 0.05–0.2 ng Mouse liver (0.5 μg): 2.5–8 ng Mouse brain (0.5 μg): 0.1–0.5 ng
Elution volume	5–30 µl
A₂₆₀/A₂₈₀	1.9–2.2
Preparation time	18 min/6 preps
Binding capacity	110 µg

Applications

RNA isolation from small amounts of cultured cells, human/animal tissue
RNA from samples stored in RNAlater
Purified RNA is suitable for:
- Real-time RT-PCR
- Northern blotting
- Primer extension
- Array technology
- RNase protection assay
- RNA-sequencing

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Fast, efficient RNA isolation from extra small samples, including single mammalian cells.

Rapid RNA isolation from extra-small samples, including single cells

NucleoSpin RNA Plus XS provides fast, reliable isolation of high-quality RNA from extra-small (XS) amounts of cells and tissues, including single mammalian cells. This kit utilizes a novel lysis buffer that does not contain ß-mercaptoethanol or TCEP, and it includes gDNA removal columns that eliminate the need for time-consuming DNA digestion. The NucleoSpin RNA Plus XS kit yields highly concentrated RNA (in an elution volume of 5–30 µl) that is ready for all typical downstream applications.

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NucleoSpin RNA Plus XS—fast, efficient RNA isolation from the smallest samples

Notice to purchaser

740990.10: NucleoSpin RNA Plus XS

Efficient removal of genomic DNA contamination with NucleoSpin RNA Plus XS

Efficient recovery of total RNA across varying input amounts with NucleoSpin RNA Plus XS

Notice to purchaser

740990.250: NucleoSpin RNA Plus XS

Efficient removal of genomic DNA contamination with NucleoSpin RNA Plus XS

Efficient recovery of total RNA across varying input amounts with NucleoSpin RNA Plus XS

Notice to purchaser

Efficient removal of genomic DNA contamination with NucleoSpin RNA Plus XS

Efficient recovery of total RNA across varying input amounts with NucleoSpin RNA Plus XS

740990.50: NucleoSpin RNA Plus XS

Overview

More Information

Applications

Additional product information