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  • Takara IVTpro mRNA Synthesis Systems
  • RNA modification
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Overview mRNA synthesis learning center
5′-capping information
Home › Products › mRNA and cDNA synthesis › In vitro transcription › Takara IVTpro mRNA Synthesis Systems

mRNA and cDNA synthesis

  • In vitro transcription
    • Takara IVTpro mRNA Synthesis Systems
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  • mRNA and cDNA synthesis accessories
    • RNase inhibitors
      • Oxidation-resistant recombinant RNase inhibitor
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    • Oligo modification
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    • ExpressHyb Hybridization Solution
    • RNase-Free Water
    • DNA Fragmentation Kit
    • Lyophilized master mix
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Overview mRNA synthesis learning center
5′-capping information

Takara IVTpro mRNA Synthesis Systems

The Takara IVTpro mRNA Synthesis Systems (Cat. # 6141, 6148 & 6131) offer a complete solution for synthesizing high-quality single-stranded mRNA from your gene of interest, with a cap structure (using CleanCap Reagent AG from TriLink Biotechnology or similar, not included) and poly(A) sequence. They contain:

  • Cloning Kit for mRNA Template (Cat. # 6143 or 6133)—enables easy cloning of a template DNA into a pre-designed In-Fusion cloning-ready plasmid system for in vitro transcription with the Takara IVTpro T7 mRNA Synthesis Kit. The plasmid supplied with Cat. # 6143 contains a Hind III site directly after the poly(A) sequence, while Cat. # 6133 contains a BspQ I site directly after the poly(A) sequence.  
  • Takara IVTpro T7 mRNA Synthesis Kit (Cat. # 6144 or 6134)—includes a highly efficient T7 RNA polymerase mix optimized for preparing high yields of mRNA through in vitro transcription. The kit also allows integration with capping systems (not included) and RNA modification with modified NTPs. The Enzyme Mix provided as part of Cat. # 6134 contains PrimeCap T7 RNA Polymerase (low dsRNA) for reduced dsRNA production. 
Cat. # Product Size Price License Quantity Details
6141 Takara IVTpro™ mRNA Synthesis System 1 Set USD $437.00

Takara IVTpro mRNA Synthesis System includes all components necessary from DNA template preparation to high-yield in vitro transcription reaction. Easily scale up to 10-fold (200 μl reaction volume) without affecting the expected mRNA yield. This kit includes the Cloning Kit for mRNA Template (Cat. # 6143) and Takara IVTpro mRNA Synthesis Kit (Cat. # 6144), which are also sold separately.

Notice to purchaser

This product is for in vitro diagnostic use. Takara products may not be resold or transferred, modified for resale or transfer, or used to manufacture commercial products without written approval from Takara Bio Inc. If you require licenses for other use, please contact us by phone at +81 77 .565. 6976 or from our website at https://www.takara-bio.co.jp/inquiry/ivd/. Your use of this product is also subject to compliance with any applicable licensing requirements described on the product web page. It is your responsibility to review, understand and adhere to any restrictions imposed by such statements. All trademarks are the property of their respective owners. Certain trademarks may not be registered in all jurisdictions.

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6141: Takara IVTpro mRNA Synthesis System

6141: Takara IVTpro mRNA Synthesis System

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Affect of CleanCap Reagent AG (3’ OMe) on RNA yield and the expression of FLuc mRNA

Affect of CleanCap Reagent AG (3’ OMe) on RNA yield and the expression of FLuc mRNA

Addition of the CleanCap Reagent AG (3’ OMe) in IVT reaction has no significant effect on mRNA yield (Panel A). HEK293T cells transfected with mRNA containing a cap structure show significatly higher protein expression compared to cells transfected with mRNA without a cap (Panel B).

Back

Increasing IVT reaction volume has no effect on RNA yield or quality.

Increasing IVT reaction volume has no effect on RNA yield or quality.

The mRNA yield was proportional to the reaction volume up to 200 µl with no significant change in mRNA concentration (Panel A). Scaling up of the IVT reaction volume does not affect the quality of RNA product (Panel B).

Back

Effect of different RNA purification methods on RNA yield and protein expression.

Effect of different RNA purification methods on RNA yield and protein expression.

Purification of RNA product using LiCl was comparable to purification with NucleoSpin column when two elutions were performed (Panel A). HEK293T cells transfected with mRNA purified using LiCl shows protein expression comparable to mRNA purified using NucleoSpin columns (Panel B).

Back

Evaluation of the performance of IVT using different template lengths (0.7 Kb–12 Kb)

 Evaluation of the performance of IVT using different template lengths (0.7 Kb–12 Kb)

The IVT reaction was performed using various sizes of DNA Templates with CleanCap Reagent AG (3' OMe) and N1-methyl pseudo UTP. Each resulting mRNA (200 ng) was denatured at 65° C for 10 min, followed by analysis using 1.2% formaldehyde gel electrophoresis.

6148 Takara IVTpro™ mRNA Synthesis System (BspQ Ⅰ) 1 Set USD $437.00

The Takara IVTpro mRNA Synthesis System (BspQ I) includes all the components necessary to produce high-quality in vitro-transcribed mRNA, from DNA template preparation to the in vitro transcription reaction. This system is supplied with a T7 cloning vector containing a BspQ I restriction site for producing scarless templates for in vitro transcription. 

Cat. # 6148 includes the Cloning Kit for mRNA Template (BspQ I) (Cat. # 6133) and Takara IVTpro mRNA Synthesis Kit (Cat. # 6144), both of which are also sold separately. 

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents
6131 Takara IVTpro™ mRNA Synthesis System (low dsRNA) 1 Set USD $481.00

The Takara IVTpro mRNA Synthesis System (low dsRNA) includes all the components necessary to produce high-quality in vitro-transcribed mRNA, from DNA template preparation to the in vitro transcription reaction. This system is supplied with Enzyme Mix containing PrimeCap T7 RNA Polymerase (low dsRNA) for reduced dsRNA production. 

Cat. # 6131 includes the Cloning Kit for mRNA Template (BspQ I) (Cat. # 6133) and Takara IVTpro mRNA Synthesis Kit (low dsRNA) (Cat. # 6134), both of which are also sold separately. 

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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6131: Takara IVTpro mRNA Synthesis System (low dsRNA)

6131: Takara IVTpro mRNA Synthesis System (low dsRNA)
6144 Takara IVTpro™ T7 mRNA Synthesis Kit 20 Rxns USD $273.00

Takara IVTpro mRNA Synthesis Kit enables the production of large amounts of mRNA through in vitro transcription using an optimized, highly efficient T7 RNA polymerase. The kit includes separate NTPs for easy optimization or replacement with modified nucleosides, such as pseudouridine. The kit synthesizes up to 200 μg or more of mRNA per 20 μl reaction, includes DNase I to digest the template DNA after the in vitro transcription reaction and a lithium chloride (LiCl) solution to purify the synthesized mRNA for downstream applications. 

The kit also includes a Positive Control Template (FLuc) plasmid containing the T7 promoter, transcription initiation sequence (AGG), 5'- and 3'-UTRs, FLuc CDS, and poly(A) sequence.

Easily scale up to 10-fold (200 μl reaction volume) without affecting the expected mRNA yield.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Affect of CleanCap Reagent AG (3’ OMe) on RNA yield and the expression of FLuc mRNA

Affect of CleanCap Reagent AG (3’ OMe) on RNA yield and the expression of FLuc mRNA

Addition of the CleanCap Reagent AG (3’ OMe) in IVT reaction has no significant effect on mRNA yield (Panel A). HEK293T cells transfected with mRNA containing a cap structure show significatly higher protein expression compared to cells transfected with mRNA without a cap (Panel B).

Back

Increasing IVT reaction volume has no effect on RNA yield or quality.

Increasing IVT reaction volume has no effect on RNA yield or quality.

The mRNA yield was proportional to the reaction volume up to 200 µl with no significant change in mRNA concentration (Panel A). Scaling up of the IVT reaction volume does not affect the quality of RNA product (Panel B).

Back

Effect of different RNA purification methods on RNA yield and protein expression.

Effect of different RNA purification methods on RNA yield and protein expression.

Purification of RNA product using LiCl was comparable to purification with NucleoSpin column when two elutions were performed (Panel A). HEK293T cells transfected with mRNA purified using LiCl shows protein expression comparable to mRNA purified using NucleoSpin columns (Panel B).

Back

6144: Takara IVTpro T7 mRNA Synthesis Kit

6144: Takara IVTpro T7 mRNA Synthesis Kit

Back

Evaluation of the performance of IVT using different template lengths (0.7 Kb–12 Kb)

 Evaluation of the performance of IVT using different template lengths (0.7 Kb–12 Kb)

The IVT reaction was performed using various sizes of DNA Templates with CleanCap Reagent AG (3' OMe) and N1-methyl pseudo UTP. Each resulting mRNA (200 ng) was denatured at 65° C for 10 min, followed by analysis using 1.2% formaldehyde gel electrophoresis.

6134 Takara IVTpro™ T7 mRNA Synthesis Kit (low dsRNA) 20 Rxns USD $300.00

The Takara IVTpro mRNA Synthesis Kit enables the production of large amounts of mRNA through in vitro transcription, synthesizing up to 200 μg or more of mRNA per 20 μl reaction.

The kit includes a highly efficient T7 RNA polymerase, separate NTPs for easy optimization or replacement with modified nucleosides, DNase I to digest the template DNA after the in vitro transcription reaction, a lithium chloride (LiCl) solution to purify the synthesized mRNA for downstream applications, and a Positive Control Template (FLuc) plasmid containing the T7 promoter, transcription initiation sequence (AGG), 5'- and 3'-UTRs, FLuc CDS, and poly(A) sequence.

Cat. # 6134 has an Enzyme Mix containing PrimeCap T7 RNA Polymerase (low dsRNA) for reduced dsRNA production.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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6134: Takara IVTpro mRNA Synthesis System (low dsRNA)

6134: Takara IVTpro mRNA Synthesis System (low dsRNA)
6143 Cloning Kit for mRNA Template 10 Rxns USD $196.00

Cloning Kit for mRNA Template enables easy cloning of a template DNA into a pre-linearized plasmid (included in the kit) for downstream in vitro transcription reactions. The pre-linearized plasmid contains the T7 promoter, transcription initiation sequence (AGG), 5'- and 3'-UTRs (untranslated region), and poly(A) sequence. The kit also includes an In-Fusion Snap Assembly Master Mix for seamless cloning of the target DNA into the pre-linearized vector and a FLuc Control Fragment, consisting of 15 bp 3' and 5' In-Fusion sequences and Fluc CDS (1,683 bp).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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6143: Cloning Kit for mRNA Template

6143: Cloning Kit for mRNA Template
6133 Cloning Kit for mRNA Template (BspQ I) 10 Rxns USD $196.00

Cloning Kit for mRNA Template enables easy cloning of a template DNA into a pre-linearized plasmid (included in the kit) for downstream in vitro transcription reactions. The kit includes an In-Fusion Snap Assembly Master Mix for seamless cloning of the target DNA into the pre-linearized vector and a FLuc Control Fragment, consisting of 15 bp 3' and 5' In-Fusion sequences and Fluc CDS (1,683 bp).

The pre-linearized plasmid that is provided as part of Cat. # 6144 contains a BspQ I digestion site immediately following the poly(A) sequence. This plasmid can be purchased separately (Cat. #6146).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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6133: Takara IVTpro mRNA Synthesis System (low dsRNA)

6133: Takara IVTpro mRNA Synthesis System (low dsRNA)

Overview

Takara IVTpro mRNA Synthesis Systems accelerate RNA-focused research with the following benefits:

  • Streamlined workflow—complete in vitro transcription workflow, from template preparation to mRNA purification, with a single kit
  • Ultra-high yield—generate up to 200 µg of mRNA per reaction with a cap structure and poly(A) sequence
  • Flexibility—incorporate a wide variety of capping systems, including CleanCap or ARCA and/or a modified nucleoside such as pseudouridine

More Information

Applications

  • Construction of plasmid DNA for in vitro transcription template
  • Capped mRNA synthesis using TriLink's CleanCap Reagent AG, or other cap analogs such as Anti-Reverse Cap Analog (ARCA)*
  • Synthesis of long non-coding RNA or siRNA precursors for non-coding RNA research
  • Synthesis of RNA standard template for RT-qPCR
  • RNA probe synthesis for hybridization

A different template sequence design is required when using ARCA.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


Takara IVTpro mRNA Synthesis Systems
Takara IVTpro mRNA Synthesis Systems include all the components necessary to produce high-quality in vitro-transcribed mRNA, from DNA template preparation to the in vitro transcription reaction.

Cat. # Product Size Price License Quantity Details
6141 Takara IVTpro™ mRNA Synthesis System 1 Set USD $437.00

Takara IVTpro mRNA Synthesis System includes all components necessary from DNA template preparation to high-yield in vitro transcription reaction. Easily scale up to 10-fold (200 μl reaction volume) without affecting the expected mRNA yield. This kit includes the Cloning Kit for mRNA Template (Cat. # 6143) and Takara IVTpro mRNA Synthesis Kit (Cat. # 6144), which are also sold separately.

Notice to purchaser

This product is for in vitro diagnostic use. Takara products may not be resold or transferred, modified for resale or transfer, or used to manufacture commercial products without written approval from Takara Bio Inc. If you require licenses for other use, please contact us by phone at +81 77 .565. 6976 or from our website at https://www.takara-bio.co.jp/inquiry/ivd/. Your use of this product is also subject to compliance with any applicable licensing requirements described on the product web page. It is your responsibility to review, understand and adhere to any restrictions imposed by such statements. All trademarks are the property of their respective owners. Certain trademarks may not be registered in all jurisdictions.

Documents Components Image Data

Back

6141: Takara IVTpro mRNA Synthesis System

6141: Takara IVTpro mRNA Synthesis System

Back

Affect of CleanCap Reagent AG (3’ OMe) on RNA yield and the expression of FLuc mRNA

Affect of CleanCap Reagent AG (3’ OMe) on RNA yield and the expression of FLuc mRNA

Addition of the CleanCap Reagent AG (3’ OMe) in IVT reaction has no significant effect on mRNA yield (Panel A). HEK293T cells transfected with mRNA containing a cap structure show significatly higher protein expression compared to cells transfected with mRNA without a cap (Panel B).

Back

Increasing IVT reaction volume has no effect on RNA yield or quality.

Increasing IVT reaction volume has no effect on RNA yield or quality.

The mRNA yield was proportional to the reaction volume up to 200 µl with no significant change in mRNA concentration (Panel A). Scaling up of the IVT reaction volume does not affect the quality of RNA product (Panel B).

Back

Effect of different RNA purification methods on RNA yield and protein expression.

Effect of different RNA purification methods on RNA yield and protein expression.

Purification of RNA product using LiCl was comparable to purification with NucleoSpin column when two elutions were performed (Panel A). HEK293T cells transfected with mRNA purified using LiCl shows protein expression comparable to mRNA purified using NucleoSpin columns (Panel B).

Back

Evaluation of the performance of IVT using different template lengths (0.7 Kb–12 Kb)

 Evaluation of the performance of IVT using different template lengths (0.7 Kb–12 Kb)

The IVT reaction was performed using various sizes of DNA Templates with CleanCap Reagent AG (3' OMe) and N1-methyl pseudo UTP. Each resulting mRNA (200 ng) was denatured at 65° C for 10 min, followed by analysis using 1.2% formaldehyde gel electrophoresis.

6148 Takara IVTpro™ mRNA Synthesis System (BspQ Ⅰ) 1 Set USD $437.00

The Takara IVTpro mRNA Synthesis System (BspQ I) includes all the components necessary to produce high-quality in vitro-transcribed mRNA, from DNA template preparation to the in vitro transcription reaction. This system is supplied with a T7 cloning vector containing a BspQ I restriction site for producing scarless templates for in vitro transcription. 

Cat. # 6148 includes the Cloning Kit for mRNA Template (BspQ I) (Cat. # 6133) and Takara IVTpro mRNA Synthesis Kit (Cat. # 6144), both of which are also sold separately. 

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents
6131 Takara IVTpro™ mRNA Synthesis System (low dsRNA) 1 Set USD $481.00

The Takara IVTpro mRNA Synthesis System (low dsRNA) includes all the components necessary to produce high-quality in vitro-transcribed mRNA, from DNA template preparation to the in vitro transcription reaction. This system is supplied with Enzyme Mix containing PrimeCap T7 RNA Polymerase (low dsRNA) for reduced dsRNA production. 

Cat. # 6131 includes the Cloning Kit for mRNA Template (BspQ I) (Cat. # 6133) and Takara IVTpro mRNA Synthesis Kit (low dsRNA) (Cat. # 6134), both of which are also sold separately. 

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

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6131: Takara IVTpro mRNA Synthesis System (low dsRNA)

6131: Takara IVTpro mRNA Synthesis System (low dsRNA)


Takara IVTpro Synthesis Kits
Takara IVTpro mRNA Synthesis Kits contain all the components necessary for producing of large amounts of mRNA through in vitro transcription, synthesizing up to 200 μg or more of mRNA per 20 μl reaction.

Cat. # Product Size Price License Quantity Details
6144 Takara IVTpro™ T7 mRNA Synthesis Kit 20 Rxns USD $273.00

Takara IVTpro mRNA Synthesis Kit enables the production of large amounts of mRNA through in vitro transcription using an optimized, highly efficient T7 RNA polymerase. The kit includes separate NTPs for easy optimization or replacement with modified nucleosides, such as pseudouridine. The kit synthesizes up to 200 μg or more of mRNA per 20 μl reaction, includes DNase I to digest the template DNA after the in vitro transcription reaction and a lithium chloride (LiCl) solution to purify the synthesized mRNA for downstream applications. 

The kit also includes a Positive Control Template (FLuc) plasmid containing the T7 promoter, transcription initiation sequence (AGG), 5'- and 3'-UTRs, FLuc CDS, and poly(A) sequence.

Easily scale up to 10-fold (200 μl reaction volume) without affecting the expected mRNA yield.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Affect of CleanCap Reagent AG (3’ OMe) on RNA yield and the expression of FLuc mRNA

Affect of CleanCap Reagent AG (3’ OMe) on RNA yield and the expression of FLuc mRNA

Addition of the CleanCap Reagent AG (3’ OMe) in IVT reaction has no significant effect on mRNA yield (Panel A). HEK293T cells transfected with mRNA containing a cap structure show significatly higher protein expression compared to cells transfected with mRNA without a cap (Panel B).

Back

Increasing IVT reaction volume has no effect on RNA yield or quality.

Increasing IVT reaction volume has no effect on RNA yield or quality.

The mRNA yield was proportional to the reaction volume up to 200 µl with no significant change in mRNA concentration (Panel A). Scaling up of the IVT reaction volume does not affect the quality of RNA product (Panel B).

Back

Effect of different RNA purification methods on RNA yield and protein expression.

Effect of different RNA purification methods on RNA yield and protein expression.

Purification of RNA product using LiCl was comparable to purification with NucleoSpin column when two elutions were performed (Panel A). HEK293T cells transfected with mRNA purified using LiCl shows protein expression comparable to mRNA purified using NucleoSpin columns (Panel B).

Back

6144: Takara IVTpro T7 mRNA Synthesis Kit

6144: Takara IVTpro T7 mRNA Synthesis Kit

Back

Evaluation of the performance of IVT using different template lengths (0.7 Kb–12 Kb)

 Evaluation of the performance of IVT using different template lengths (0.7 Kb–12 Kb)

The IVT reaction was performed using various sizes of DNA Templates with CleanCap Reagent AG (3' OMe) and N1-methyl pseudo UTP. Each resulting mRNA (200 ng) was denatured at 65° C for 10 min, followed by analysis using 1.2% formaldehyde gel electrophoresis.

6134 Takara IVTpro™ T7 mRNA Synthesis Kit (low dsRNA) 20 Rxns USD $300.00

The Takara IVTpro mRNA Synthesis Kit enables the production of large amounts of mRNA through in vitro transcription, synthesizing up to 200 μg or more of mRNA per 20 μl reaction.

The kit includes a highly efficient T7 RNA polymerase, separate NTPs for easy optimization or replacement with modified nucleosides, DNase I to digest the template DNA after the in vitro transcription reaction, a lithium chloride (LiCl) solution to purify the synthesized mRNA for downstream applications, and a Positive Control Template (FLuc) plasmid containing the T7 promoter, transcription initiation sequence (AGG), 5'- and 3'-UTRs, FLuc CDS, and poly(A) sequence.

Cat. # 6134 has an Enzyme Mix containing PrimeCap T7 RNA Polymerase (low dsRNA) for reduced dsRNA production.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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6134: Takara IVTpro mRNA Synthesis System (low dsRNA)

6134: Takara IVTpro mRNA Synthesis System (low dsRNA)


Cloning Kits for mRNA Templates
Cloning Kits for mRNA Templates enable easy cloning of a template DNA into a pre-linearized plasmid (included in the kit) for downstream in vitro transcription reactions.

Cat. # Product Size Price License Quantity Details
6143 Cloning Kit for mRNA Template 10 Rxns USD $196.00

Cloning Kit for mRNA Template enables easy cloning of a template DNA into a pre-linearized plasmid (included in the kit) for downstream in vitro transcription reactions. The pre-linearized plasmid contains the T7 promoter, transcription initiation sequence (AGG), 5'- and 3'-UTRs (untranslated region), and poly(A) sequence. The kit also includes an In-Fusion Snap Assembly Master Mix for seamless cloning of the target DNA into the pre-linearized vector and a FLuc Control Fragment, consisting of 15 bp 3' and 5' In-Fusion sequences and Fluc CDS (1,683 bp).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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6143: Cloning Kit for mRNA Template

6143: Cloning Kit for mRNA Template
6133 Cloning Kit for mRNA Template (BspQ I) 10 Rxns USD $196.00

Cloning Kit for mRNA Template enables easy cloning of a template DNA into a pre-linearized plasmid (included in the kit) for downstream in vitro transcription reactions. The kit includes an In-Fusion Snap Assembly Master Mix for seamless cloning of the target DNA into the pre-linearized vector and a FLuc Control Fragment, consisting of 15 bp 3' and 5' In-Fusion sequences and Fluc CDS (1,683 bp).

The pre-linearized plasmid that is provided as part of Cat. # 6144 contains a BspQ I digestion site immediately following the poly(A) sequence. This plasmid can be purchased separately (Cat. #6146).

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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6133: Takara IVTpro mRNA Synthesis System (low dsRNA)

6133: Takara IVTpro mRNA Synthesis System (low dsRNA)


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mRNA synthesis learning center

Explore Takara IVTpro products and learn how our technology can support your mRNA therapeutic research. This learning center includes vector information, different 5′-capping compatibility, a link to download a flyer, and more.

Learn more

T7 RNA Polymerase

The enhanced activity of T7 RNA Polymerase ver.2.0 enables the production of large quantities of high-quality RNA with high processivity and transcription frequency.

Inorganic Pyrophosphatase

Use our inorganic pyrophosphatase to improve the RNA yield in in vitro transcription reactions.

Vaccinia Capping Enzyme

Vaccinia Capping Enzyme adds Cap-0 structures to the 5′ ends of uncapped, in vitro-transcribed RNA.

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United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2025 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

Takara Bio

Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Clontech, TaKaRa, cellartis

  • Products
  • COVID-19 research
  • Next-generation sequencing
  • Real-time PCR
  • Stem cell research
  • mRNA and cDNA synthesis
  • PCR
  • Cloning
  • Nucleic acid purification
  • Gene function
  • Protein research
  • Antibodies and ELISA
  • New products
  • Special offers
  • COVID-19 research
  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Viral and host sequencing
  • Vaccine development
  • CRISPR screening
  • Drug discovery
  • Immune profiling
  • Publications
  • Next-generation sequencing
  • Spatial omics
  • RNA-seq
  • DNA-seq
  • Single-cell NGS automation
  • Reproductive health
  • Bioinformatics tools
  • Immune profiling
  • Real-time PCR
  • Great value master mixes
  • Signature enzymes
  • High-throughput real-time PCR solutions
  • Detection assays
  • References, standards, and buffers
  • Stem cell research
  • Media, differentiation kits, and matrices
  • Stem cells and stem cell-derived cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
  • Restriction enzymes
  • Nucleic acid purification
  • Automated platforms
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • Gene function
  • Gene editing
  • Viral transduction
  • Fluorescent proteins
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • Transfection reagents
  • Cell biology assays
  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Antibodies and ELISA
  • Primary antibodies and ELISAs by research area
  • Fluorescent protein antibodies
  • Services & Support
  • OEM
  • Instrument services
  • Gene and cell therapy manufacturing
  • Customer service
  • Sales
  • Shipping & delivery
  • Technical support
  • Feedback
  • Online tools
  • Partnering & Licensing
  • Vector information
  • OEM
  • Portfolio
  • Process
  • Facilities
  • Request samples
  • FAQs
  • Instrument services
  • Apollo services
  • ICELL8 services
  • SmartChip ND system services
  • Gene and cell therapy manufacturing
  • Services
  • Facilities
  • Our process
  • Resources
  • Sales
  • Make an appointment with your sales rep
  • Online tools
  • GoStix Plus FAQs
  • Vector information
  • Vector document overview
  • Vector document finder
  • Learning centers
  • Automation systems
  • Next-generation sequencing
  • Spatial biology
  • Real-time PCR
  • Nucleic acid purification
  • mRNA and cDNA synthesis
  • PCR
  • Cloning
  • Stem cell research
  • Gene function
  • Protein research
  • Antibodies and ELISA
  • Automation systems
  • Shasta Single Cell System introduction
  • SmartChip Real-Time PCR System introduction
  • ICELL8 introduction
  • Next-generation sequencing
  • RNA-seq
  • Technical notes
  • Technology and application overviews
  • FAQs and tips
  • DNA-seq protocols
  • Bioinformatics resources
  • Webinars
  • Real-time PCR
  • Download qPCR resources
  • Overview
  • Reaction size guidelines
  • Guest webinar: extraction-free SARS-CoV-2 detection
  • Technical notes
  • Nucleic acid purification
  • Nucleic acid extraction webinars
  • Product demonstration videos
  • Product finder
  • Plasmid kit selection guide
  • RNA purification kit finder
  • mRNA and cDNA synthesis
  • mRNA synthesis
  • cDNA synthesis
  • PCR
  • Citations
  • PCR selection guide
  • Technical notes
  • FAQ
  • Cloning
  • Automated In-Fusion Cloning
  • In-Fusion Cloning general information
  • Primer design and other tools
  • In‑Fusion Cloning tips and FAQs
  • Applications and technical notes
  • Stem cell research
  • Overview
  • Protocols
  • Technical notes
  • Gene function
  • Gene editing
  • Viral transduction
  • T-cell transduction and culture
  • Inducible systems
  • Cell biology assays
  • Protein research
  • Capturem technology
  • Antibody immunoprecipitation
  • His-tag purification
  • Other tag purification
  • Expression systems
  • APPLICATIONS
  • Molecular diagnostics
  • mRNA and protein therapeutics
  • Pathogen detection
  • Immunotherapy research
  • Cancer research
  • Alzheimer's disease research
  • Reproductive health technologies
  • Infectious diseases
  • Molecular diagnostics
  • Interview: adapting to change with Takara Bio
  • Applications
  • Solutions
  • Partnering
  • Contact us
  • mRNA and protein therapeutics
  • Characterizing the viral genome and host response
  • Identifying and cloning protein targets
  • Expressing and purifying protein targets
  • Immunizing mice and optimizing vaccines
  • Pathogen detection
  • Sample prep
  • Detection methods
  • Identification and characterization
  • SARS-CoV-2
  • Antibiotic-resistant bacteria
  • Food crop pathogens
  • Waterborne disease outbreaks
  • Viral-induced cancer
  • Immunotherapy research
  • T-cell therapy
  • Antibody therapeutics
  • T-cell receptor profiling
  • TBI initiatives in cancer therapy
  • Cancer research
  • Kickstart your cancer research with long-read sequencing
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer transcriptome analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
  • Alzheimer's disease research
  • Antibody engineering
  • Sample prep from FFPE tissue
  • Single-cell sequencing
  • Reproductive health technologies
  • Embgenix FAQs
  • Preimplantation genetic testing
  • ESM partnership program
  • ESM Collection Kit forms
  • Infectious diseases
  • Develop vaccines for HIV
  • About
  • BioView blog
  • That's Good Science!
  • Our brands
  • Our history
  • In the news
  • Events
  • Careers
  • Trademarks
  • License statements
  • Quality and compliance
  • HQ-grade reagents
  • International Contacts by Region
  • Need help?
  • Website FAQs
  • BioView blog
  • Automation
  • Cancer research
  • Career spotlights
  • Current events
  • Customer stories
  • Gene editing
  • Research news
  • Single-cell analysis
  • Stem cell research
  • Tips and troubleshooting
  • Women in STEM
  • That's Good Support!
  • About our blog
  • That's Good Science!
  • SMART-Seq Pro Biomarker Discovery Contest
  • DNA extraction educational activity
  • That's Good Science Podcast
  • Season one
  • Season two
  • Season three
  • Events
  • Biomarker discovery events
  • Calendar
  • Conferences
  • Speak with us
  • Careers
  • Company benefits
  • International Contacts by Region
  • United States and Canada
  • China
  • Japan
  • Korea
  • Europe
  • India
  • Affiliates & distributors
  • Need help?
  • Privacy request
Takara Bio
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