Takara IVTpro mRNA Synthesis System

Takara IVTpro mRNA Synthesis System offers a complete solution for synthesizing high-quality single-stranded mRNA from your gene of interest, with a cap structure (using CleanCap Reagent AG from TriLink Biotechnology or similar, not included) and poly(A) sequence. The Takara IVTpro mRNA Synthesis System (Cat. # 6141) comprises:

Cloning Kit for mRNA Template (Cat. # 6143)—enables easy cloning of a template DNA into a pre-designed In-Fusion cloning-ready plasmid system for in vitro transcription with the Takara IVTpro T7 mRNA Synthesis Kit.
Takara IVTpro T7 mRNA Synthesis Kit (Cat. # 6144)—includes a highly-efficient T7 RNA polymerase optimized for preparing high yields of mRNA through in vitro transcription. The kit also allows integration with capping systems (not included) and RNA modification with modified NTPs.

Cat. #	Product	Size
6141	Takara IVTpro™ mRNA Synthesis System	1 Set	USD $398.00
Takara IVTpro mRNA Synthesis System includes all components necessary from DNA template preparation to high-yield in vitro transcription reaction. Easily scale up to 10-fold (200 μl reaction volume) without affecting the expected mRNA yield. This kit includes the Cloning Kit for mRNA Template (Cat. # 6143) and Takara IVTpro mRNA Synthesis Kit (Cat. # 6144), which are also sold separately. Notice to purchaser This product is for in vitro diagnostic use. Takara products may not be resold or transferred, modified for resale or transfer, or used to manufacture commercial products without written approval from Takara Bio Inc. If you require licenses for other use, please contact us by phone at +81 77 .565. 6976 or from our website at https://www.takara-bio.co.jp/inquiry/ivd/. Your use of this product is also subject to compliance with any applicable licensing requirements described on the product web page. It is your responsibility to review, understand and adhere to any restrictions imposed by such statements. All trademarks are the property of their respective owners. Certain trademarks may not be registered in all jurisdictions. Documents Components Image Data Back Affect of CleanCap Reagent AG (3’ OMe) on RNA yield and the expression of FLuc mRNA Addition of the CleanCap Reagent AG (3’ OMe) in IVT reaction has no significant effect on mRNA yield (Panel A). HEK293T cells transfected with mRNA containing a cap structure show significatly higher protein expression compared to cells transfected with mRNA without a cap (Panel B). Back Increasing IVT reaction volume has no effect on RNA yield or quality. The mRNA yield was proportional to the reaction volume up to 200 µl with no significant change in mRNA concentration (Panel A). Scaling up of the IVT reaction volume does not affect the quality of RNA product (Panel B). Back Effect of different RNA purification methods on RNA yield and protein expression. Purification of RNA product using LiCl was comparable to purification with NucleoSpin column when two elutions were performed (Panel A). HEK293T cells transfected with mRNA purified using LiCl shows protein expression comparable to mRNA purified using NucleoSpin columns (Panel B). Back 6141: Takara IVTpro mRNA Synthesis System
6143	Cloning Kit for mRNA Template	10 Rxns	USD $179.00
Cloning Kit for mRNA Template enables easy cloning of a template DNA into a pre-linearized plasmid (included in the kit) for downstream in vitro transcription reactions. The pre-linearized plasmid contains the T7 promoter, transcription initiation sequence (AGG), 5'- and 3'-UTRs (untranslated region), and poly(A) sequence. The kit also includes an In-Fusion Snap Assembly Master Mix for seamless cloning of the target DNA into the pre-linearized vector and a FLuc Control Fragment, consisting of 15 bp 3' and 5' In-Fusion sequences and Fluc CDS (1,683 bp). Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 6143: Cloning Kit for mRNA Template
6144	Takara IVTpro™ T7 mRNA Synthesis Kit	20 Rxns	USD $248.00
Takara IVTpro mRNA Synthesis Kit enables the production of large amounts of mRNA through in vitro transcription using an optimized, highly efficient T7 RNA polymerase. The kit includes separate NTPs for easy optimization or replacement with modified nucleosides, such as pseudouridine. The kit synthesizes up to 200 μg or more of mRNA per 20 μl reaction, includes DNase I to digest the template DNA after the in vitro transcription reaction and a lithium chloride (LiCl) solution to purify the synthesized mRNA for downstream applications. The kit also includes a Positive Control Template (FLuc) plasmid containing the T7 promoter, transcription initiation sequence (AGG), 5'- and 3'-UTRs, FLuc CDS, and poly(A) sequence. Easily scale up to 10-fold (200 μl reaction volume) without affecting the expected mRNA yield. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Affect of CleanCap Reagent AG (3’ OMe) on RNA yield and the expression of FLuc mRNA Addition of the CleanCap Reagent AG (3’ OMe) in IVT reaction has no significant effect on mRNA yield (Panel A). HEK293T cells transfected with mRNA containing a cap structure show significatly higher protein expression compared to cells transfected with mRNA without a cap (Panel B). Back Increasing IVT reaction volume has no effect on RNA yield or quality. The mRNA yield was proportional to the reaction volume up to 200 µl with no significant change in mRNA concentration (Panel A). Scaling up of the IVT reaction volume does not affect the quality of RNA product (Panel B). Back Effect of different RNA purification methods on RNA yield and protein expression. Purification of RNA product using LiCl was comparable to purification with NucleoSpin column when two elutions were performed (Panel A). HEK293T cells transfected with mRNA purified using LiCl shows protein expression comparable to mRNA purified using NucleoSpin columns (Panel B). Back 6144: Takara IVTpro T7 mRNA Synthesis Kit

Overview

The Takara IVTpro mRNA Synthesis System accelerates RNA-focused research with the following benefits:

Streamlined workflow—complete in vitro transcription workflow, from template preparation to mRNA purification, with a single kit
Ultra-high yield—generate up to 200 µg of mRNA per reaction with a cap structure and poly(A) sequence
Flexibility—incorporate a wide variety of capping systems, including CleanCap or ARCA and/or a modified nucleoside such as pseudouridine

More Information

Applications

Construction of plasmid DNA for in vitro transcription template
Capped mRNA synthesis using TriLink's CleanCap Reagent AG, or other cap analogs such as Anti-Reverse Cap Analog (ARCA)*
Synthesis of long non-coding RNA or siRNA precursors for non-coding RNA research
Synthesis of RNA standard template for RT-qPCR
RNA probe synthesis for hybridization

*A different template sequence design is required when using ARCA.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

mRNA synthesis learning center

Explore Takara IVTpro products and learn how our technology can support your RNA research. This learning center includes vector information, different 5′-capping compatibility, a link to download a flyer, and more.

Learn more

T7 RNA Polymerase

The enhanced activity of T7 RNA Polymerase ver.2.0 enables the production of large quantities of high-quality RNA with high processivity and transcription frequency.

Inorganic Pyrophosphatase

Use our inorganic pyrophosphatase to improve the RNA yield in in vitro transcription reactions.

Vaccinia Capping Enzyme

Vaccinia Capping Enzyme adds Cap-0 structures to the 5′ ends of uncapped, in vitro-transcribed RNA.

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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2023 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

Takara IVTpro mRNA Synthesis System

Notice to purchaser

Affect of CleanCap Reagent AG (3’ OMe) on RNA yield and the expression of FLuc mRNA

Increasing IVT reaction volume has no effect on RNA yield or quality.

Effect of different RNA purification methods on RNA yield and protein expression.

6141: Takara IVTpro mRNA Synthesis System

Notice to purchaser

6143: Cloning Kit for mRNA Template

Notice to purchaser

Affect of CleanCap Reagent AG (3’ OMe) on RNA yield and the expression of FLuc mRNA

Increasing IVT reaction volume has no effect on RNA yield or quality.

Effect of different RNA purification methods on RNA yield and protein expression.

6144: Takara IVTpro T7 mRNA Synthesis Kit

Overview

More Information

Applications

Additional product information