- cDNA synthesis kits
- Reverse transcriptases
- RACE kits
- Purified cDNA & genomic DNA
- Purified total RNA and mRNA
- cDNA synthesis accessories
RNA LA PCR Kit
The RNA LA PCR Kit (AMV) Ver. 1 is a two-step RT-PCR kit designed for cDNA synthesis and amplification from longer templates with high accuracy. The kit uses AMV RT XL as the reverse transcriptase, allowing for the efficient synthesis of first-strand cDNA up to 12 kb in length. AMV RT XL also has better thermostability than other reverse transcriptases and retains its activity over a wider range of reaction temperatures. For cDNA amplification, the kit uses Takara LA Taq polymerase, which can generate amplicons up to 40 kb in size and at 6.5X better fidelity than Taq polymerase. Both the reverse transcription and amplification reactions can be performed in a single tube. Reaction components for 3'-RACE are also supplied in the RNA LA PCR Kit.
- Enables first-strand cDNA synthesis from templates up to 12 kb in length
- Two-step RT-PCR using AMV RT XL and reverse transcriptase
- Amplification of longer cDNA templates following reverse transcription using Takara LA Taq
- AMV Reverse Transcriptase XL* (5 U/µl; 50 µl)
- RNase Inhibitor (40 U/µl; 60 µl)
- Random 9-mers (50 pmol/µl; 50 µl)
- Oligo(dT)20-M4 Adaptor Primer (2.5 pmol/µl; 50 µl)
- RNase-Free dH2O (DEPC-treated) (1 ml)
- Takara LA Taq (5 U/µl; 25 µl)
- M13 Primer M4 (20 pmol/µl; 50 µl)
- 10X RNA PCR Buffer (120 µl)
- 10X LA Buffer II (without Mg2+) (500 µl)
- dNTP Mixture (10 mM each; 150 µl)
- MgCl2 (25 mM; 1 ml)
- Control Primer R-1 (20 pmol/µl; 25 µl)
- Control Primer R-2 (20 pmol/µl; 25 µl)
- Positive Control RNA (2 x 105 copies/µl; 25 µl)
*Manufactured by Life Science Co.
Barnes, W. M. PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates. Proc. Natl. Acad. Sci. U. S. A. 91, 2216–20 (1994).
Cheng, S., Chang, S. Y., Gravitt, P. & Respess, R. Long PCR. Nature 369, 684–5 (1994).
Cheng, S., Higuchi, R. & Stoneking, M. Complete mitochondrial genome amplification. Nat. Genet. 7, 350–1 (1994).
Frohman, M. A., Dush, M. K. & Martin, G. R. Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc. Natl. Acad. Sci. U. S. A. 85, 8998–9002 (1988).
Gyllensten, U. B. & Erlich, H. A. Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus. Proc. Natl. Acad. Sci. U. S. A. 85, 7652–6 (1988).
Kawasaki, E. S. et al. Diagnosis of chronic myeloid and acute lymphocytic leukemias by detection of leukemia-specific mRNA sequences amplified in vitro. Proc. Natl. Acad. Sci. U. S. A. 85, 5698–702 (1988).
Lawyer, F. C. et al. Isolation, characterization, and expression in Escherichia coli of the DNA polymerase gene from Thermus aquaticus. J. Biol. Chem. 264, 6427–37 (1989).
Lynas, C., Cook, S. D., Laycock, K. A., Bradfield, J. W. & Maitland, N. J. Detection of latent virus mRNA in tissues using the polymerase chain reaction. J. Pathol. 157, 285–9 (1989).
Saiki, R. K. et al. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239, 487–91 (1988).
Scharf, S. J., Horn, G. T. & Erlich, H. A. Direct cloning and sequence analysis of enzymatically amplified genomic sequences. Science 233, 1076–8 (1986).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2019 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.