- cDNA synthesis kits
- Reverse transcriptases
- RACE kits
- Purified cDNA & genomic DNA
- Purified total RNA and mRNA
- cDNA synthesis accessories
RNA LA PCR Kit
The RNA LA PCR Kit (AMV) Ver. 1 is a two-step RT-PCR kit designed for cDNA synthesis and amplification from longer templates with high accuracy. The kit uses AMV RT XL as the reverse transcriptase, allowing for the efficient synthesis of first-strand cDNA up to 12 kb in length. AMV RT XL also has better thermostability than other reverse transcriptases and retains its activity over a wider range of reaction temperatures. For cDNA amplification, the kit uses Takara LA Taq polymerase, which can generate amplicons up to 40 kb in size and at 6.5X better fidelity than Taq polymerase. Both the reverse transcription and amplification reactions can be performed in a single tube. Reaction components for 3'-RACE are also supplied in the RNA LA PCR Kit.
- Enables first-strand cDNA synthesis from templates up to 12 kb in length
- Two-step RT-PCR using AMV RT XL and reverse transcriptase
- Amplification of longer cDNA templates following reverse transcription using Takara LA Taq
- AMV Reverse Transcriptase XL* (5 U/µl; 50 µl)
- RNase Inhibitor (40 U/µl; 60 µl)
- Random 9-mers (50 pmol/µl; 50 µl)
- Oligo(dT)20-M4 Adaptor Primer (2.5 pmol/µl; 50 µl)
- RNase-Free dH2O (DEPC-treated) (1 ml)
- Takara LA Taq (5 U/µl; 25 µl)
- M13 Primer M4 (20 pmol/µl; 50 µl)
- 10X RNA PCR Buffer (120 µl)
- 10X LA Buffer II (without Mg2+) (500 µl)
- dNTP Mixture (10 mM each; 150 µl)
- MgCl2 (25 mM; 1 ml)
- Control Primer R-1 (20 pmol/µl; 25 µl)
- Control Primer R-2 (20 pmol/µl; 25 µl)
- Positive Control RNA (2 x 105 copies/µl; 25 µl)
*Manufactured by Life Science Co.
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Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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