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  • ‹ Back to Genomic DNA purification
  • NucleoSpin Tissue
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Purification kits for isolating DNA from cultured cells, blood, tissue, plants and hard to lyse samples such as stool and soil Genomic DNA purification kits
Home › Learning centers › Nucleic acid purification › Genomic DNA purification › NucleoSpin Tissue XS

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Use the dropdown list to select the nucleic acid purification kit for your application Product finder
Purification kits for isolating DNA from cultured cells, blood, tissue, plants and hard to lyse samples such as stool and soil Genomic DNA purification kits
Product overview

Rapid purification of genomic, bacterial, and viral DNA from extra-small samples

  • Highly sensitive DNA isolation from small samples, such as biopsy materials, forensic samples, or FACS-sorted cells
  • Elution in as little as 5 μl, resulting in concentrated DNA
  • Excellent DNA recovery and purity for reliable results
  • DNA is ready to use for multiplex PCR and all common downstream applications
Column design Membrane diameter Murine liver data FFPE tissue data

Column design  

NucleoSpin Tissue XS is designed for DNA isolation from extra-small samples, such as microdissected tissue, small blood samples, or small amounts of cells. Based on reliable silica membrane technology, NucleoSpin Tissue XS overcomes the limitations of conventional kits with an innovative new column design:

A funnel-shaped ring holds a silica membrane with a very small diameter

Figure 1. A funnel-shaped ring holds a silica membrane with a very small diameter. The exceptionally small (2.0 mm) diameter of the NucleoSpin Tissue XS membrane allows efficient elution in just 5–30 μl, resulting in high DNA concentrations and a purification product that is ready for all typical downstream applications. High DNA yields and small elution volumes result in highly concentrated DNA, free of impurities and suitable for sensitive downstream applications.

Membrane diameter  

Elution volume depends on membrane diameter

Figure 2. Elution volume depends on membrane diameter. The extra-small diameter of the NucleoSpin Tissue XS membrane compared to that of competitors allows for efficient elution in just 5–30 μl, yielding a more concentrated eluate. The kit provided by Competitor Q (designed for very small samples) lacks these optimized column features, requiring elution in higher volumes (20–100 μl).

Murine liver data  

Higher DNA concentrations and total yield with NucleoSpin Tissue XS than a leading competitor

Figure 3. Higher DNA concentrations and total yield can be obtained with NucleoSpin Tissue XS than the leading competitor kit. Genomic DNA was purified from small amounts of murine liver tissue using NucleoSpin Tissue XS and a competitor's kit specially designed for very small sample amounts. With both kits, DNA was eluted in 20 μl of elution buffer. The DNA was subsequently subjected to LightCycler analysis. NucleoSpin Tissue XS was superior in both DNA concentration (Panel A) and total yield (Panel B). With NucleoSpin Tissue XS, the DNA concentration can be further increased by using smaller elution volumes down to 5 μl. The smallest default elution volume for the competitor kit is 20 μl.

FFPE tissue data  

DNA purification from microdissected FFPE tissue

Figure 4. DNA purification from microdissected, formalin-fixed, paraffin-embedded (FFPE) tissue. DNA was purified from microdissected FFPE colon cancer tissue with NucleoSpin Tissue XS. Sample sizes were 1 mm2, 0.5 mm2, 0.25 mm2, 0.125 mm2, and 0.065 mm2 of a 4-μm slice. The isolated DNA was used directly for PCR with primers specific to PAH and K-ras. NucleoSpin Tissue XS allowed purification of amplifiable DNA even from very small samples of this FFPE tissue. Data kindly provided by S. Staeck, Dr. D. Kuester, Dr. A. Poehlmann and Prof. R. Schneider-Stock, Department of Pathologie, Molecular Genetics, University of Magdeburg, Germany.

Ordering Information

Cat. # Product Size Price License Quantity Details
740901.250 NucleoSpin® Tissue XS 250 Preps $910.00

NucleoSpin Tissue XS (250) 250 preps for the isolation of genomic DNA from tissue - NucleoSpin Tissue XS Columns, Collection Tubes, buffers, Proteinase K

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes

NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes

NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes. Panel A. The columns are optimized to hold a very small silica membrane, yet fit inside a standard 1.5 ml microfuge tube. Panel B. The exceptionally small (2.0 mm) diameter of the NucleoSpinXS membrane (Column MN) allows efficient elution in only 5–30 μl. It provides a significant advantage over the small-sample column offered by Competitor Q (Column Q; 4.8 mm diameter), which requires an elution volume of at least 10–30 μl.

740901.50 NucleoSpin® Tissue XS 50 Preps $195.00

NucleoSpin Tissue XS (50) 50 preps for the isolation of genomic DNA from tissue - NucleoSpin Tissue XS Columns, Collection Tubes, buffers, Proteinase K

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

Back

NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes

NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes

NucleoSpin XS columns are designed for efficient elution of DNA/RNA in small volumes. Panel A. The columns are optimized to hold a very small silica membrane, yet fit inside a standard 1.5 ml microfuge tube. Panel B. The exceptionally small (2.0 mm) diameter of the NucleoSpinXS membrane (Column MN) allows efficient elution in only 5–30 μl. It provides a significant advantage over the small-sample column offered by Competitor Q (Column Q; 4.8 mm diameter), which requires an elution volume of at least 10–30 μl.

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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Capturem Trypsin for a rapid, efficient mass spectometry workflow at room temperature.

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Capturem Trypsin provides rapid, efficient, and complete digestion of protein samples, allowing an uninterrupted mass spectometry workflow at room temperature for downstream protein analysis. This product utilizes our novel Capturem technology in a spin column format with membrane-immobilized trypsin. Capturem Trypsin Columns may be used to completely digest protein samples in less than a minute with digestion efficiencies (protein coverage) comparable to or better than those obtained using in-solution trypsin digestion.

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