BspQ I

BspQ I creates linearized, scarless templates from plasmid DNA suitable for use in in vitro transcription. As a type IIS restriction enzyme that cleaves DNA outside its recognition site, the use of BspQ I to linearize plasmid DNA ensures that the 3′ end of the in vitro transcription template does not contain extra base pairs from the restriction site.

BspQ I creates linearized, scarless templates from plasmid DNA suitable for in in vitro transcription. As a type IIS restriction enzyme that cleaves DNA outside its recognition site, the use of BspQ I to linearize plasmid DNA ensures that the 3′ end of the in vitro transcription template does not contain extra base pairs from the restriction site. This keeps the template poly(A) sequence intact and promotes successful translation after mRNA synthesis.

BspQ I, HQ (high quality) has been manufactured with stricter quality standards and is supplied in a higher concentration and volume scaling up your processes. It is free from human- or animal-derived materials, as well as free from β-lactam compounds in the final formulation.

Do you want to explore OEM options? Contact OEM Services & Support at oem@takarabio.com.

For more information on the differences between our research, HQ, and GMP grade enzymes, please refer to the HQ-grade reagents page.

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Cat. #	Product	Size	Price	Quantity
1227A	BspQ I	500 U	USD $96.00
BspQ I is a type IIS restriction enzyme that creates linearized, scarless templates from plasmid DNA that are suitable for in vitro transcription. BspQ I (10 U/μl) and 10X BspQ I Buffer are included. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back BspQ I Restriction Site BspQ I cuts outside of its recognition site, which keeps the poly(A) tail sequence intact. Back 1227A: BspQ I
1227B	BspQ I	2,500 U	USD $429.00
BspQ I is a type IIS restriction enzyme that creates linearized, scarless templates from plasmid DNA that are suitable for in vitro transcription. BspQ I (10 U/μl) and 10X BspQ I Buffer are included. Cat. # 1227B contains 5 of Cat. # 1227A. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 1227B: BspQ I Back BspQ I Restriction Site BspQ I cuts outside of its recognition site, which keeps the poly(A) tail sequence intact.
1228A	BspQ I, HQ	20,000 U	Inquire for Quotation	*
BspQ I, HQ is a type IIS restriction enzyme that creates linearized, scarless plasmid DNA templates suitable for in vitro transcription. BspQ I, HQ (20 U/μl) and 10X BspQ I Buffer are included. BspQ I, HQ (high quality) has equivalent enzyme performance as BspQ I but has been produced with stricter quality standards and is supplied in a higher volume. It is intended for use in the preparation of active pharmaceutical ingredients (APIs) for non-clinical trials, as well as for the process development of drug manufacturing under good manufacturing practice (GMP) regulations Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components

*You must be logged in to a Purchasing Account in order to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@takarabio.com.

Overview

Perfect for preparing scarless in-vitro transcription templates from plasmid DNA

Cuts outside recognition site keeping the poly(A) tail sequence intact, which promotes successful translation downstream of the in vitro transcription
Linearizes plasmid DNA in one hour
Comes in an HQ version to support a smooth transition from RUO to GMP grade RNA manufacturing

More Information

Applications

Linearization of plasmid DNA for use as a template for in vitro transcription.

Source

Escherichia coli carrying the plasmid containing the gene for BspQ I.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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BspQ I

Notice to purchaser

BspQ I Restriction Site

1227A: BspQ I

Notice to purchaser

1227B: BspQ I

BspQ I Restriction Site

Notice to purchaser

Overview

Perfect for preparing scarless in-vitro transcription templates from plasmid DNA

More Information

Applications

Source

Additional product information