High-throughput total RNA isolation & purification—NucleoSpin 96 RNA
NucleoSpin 96 RNA is designed for automated isolation and purification of total RNA from animal or human cell cultures and tissue. With the NucleoSpin 96 RNA method, cells are lysed in the presence of chaotropic salts. The lysis buffer immediately inactivates RNases, which are present in virtually any biological material, and creates appropriate conditions for RNA to bind to the silica membrane. Genomic DNA is removed by performing on-column DNA digestion using the rDNase provided in the kit...
NucleoSpin 96 RNA is designed for automated isolation and purification of total RNA from animal or human cell cultures and tissue. With the NucleoSpin 96 RNA method, cells are lysed in the presence of chaotropic salts. The lysis buffer immediately inactivates RNases, which are present in virtually any biological material, and creates appropriate conditions for RNA to bind to the silica membrane. Genomic DNA is removed by performing on-column DNA digestion using the rDNase provided in the kit. Ready-to-run protocols are available and can be customized to fit your needs. NucleoSpin 96 RNA provides consistent RNA quality, reproducible yields of RNA, and high-throughput procedures free of cross-contamination without the use of organic extraction (e.g., Trizol). These kits are powerful tools for applications such as microarray analysis in respect to gene expression profiling, and drug discovery.
The kits allow isolation and purification of total RNA from up to 107 cells or up to 30 mg of tissue (centrifuge processing). Contaminating DNA, which is also bound to the silica membrane, is removed by on-column DNA digestion using DNase I (DNase I is supplied with the kits). Salts, metabolites, and macromolecular cellular components are removed by simple washing steps with three different buffers. Pure RNA is finally eluted under low ionic strength conditions with RNase-free water (pH 8.0–8.5).
Overview
- High-throughput total RNA isolation and purification: 96-well plates
- Fast and convenient sample processing with rDNase included in the kits
- Reproducible yields for a wide variety of samples
- Suitable for manual and automated processing, under vacuum or centrifugation
- Innovative MN wash plate minimizes risk of cross-contamination
- No organic extraction (e.g., Trizol) or precipitation
More Information
| Technology | Silica membrane |
| Format | 96-well plates |
| Processing | Manual or automated, vacuum or centrifugation |
| Starting material | <107 cultured cells 30 mg tissue |
| Fragment size | 200 bp–20 kb |
| Typical yield | <100 µg |
| Elution volume | 50–130 µl |
| Preparation time | 70 min/plate |
Applications
Manual or automated isolation and purification of total RNA from cultured cells and tissue.
Application notes
The NucleoSpin 8/96 RNA kits are compatible with many major robotic systems. Application notes are available for:
- Eppendorf epMotion 5075
- CyBi-RoboSpense
- X-Tractor Gene
- AVISO Theonyx
- Hamilton MICROLAB STAR
- Tecan Freedom
- Tecan Genesis / Te-Mo 96
- MWG RoboSeq 4204
Additional product information
Please see the product’s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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The better way to isolate RNA
Critical factors when purifying RNA are the complete removal of contaminating DNA (gDNA) and the immediate prevention of degradation of RNA. The NucleoSpin RNA Plus kit introduces the NucleoSpin gDNA Removal Column, a spin column which quickly and completely removes genomic DNA contamination without the need for DNase digestion. To maintain RNA integrity, cells and tissues are first lysed by incubation in a chaotropic ion lysis buffer solution, which immediately inactivates RNases.
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