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  • ‹ Back to Parallel RNA/DNA/protein
  • NucleoBond RNA/DNA
  • NucleoSpin RNA/Protein
  • NucleoSpin TriPrep
Overviews Parallel RNA/Protein purification
Use the dropdown list to select the nucleic acid purification kit for your application Product finder
Isolate gDNA in parallel Adapt this kit to purify DNA in parallel
Home › Products › Nucleic acid purification › RNA purification kits › Parallel RNA/DNA/protein › NucleoSpin RNA/Protein

RNA purification kits

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Overviews Parallel RNA/Protein purification
Use the dropdown list to select the nucleic acid purification kit for your application Product finder
Isolate gDNA in parallel Adapt this kit to purify DNA in parallel

NucleoSpin RNA/Protein—isolation and purification of RNA and protein from ONE lysate

Co-purification of total RNA and protein from cells and tissue

NucleoSpin RNA/Protein is a complete mini kit with recombinant DNase and shredders for rapid, high-yield isolation and purification of total RNA and protein from cells and tissue. This kit allows simultaneous isolation of RNA and protein from a single lysate in one working procedure, from a wide variety of starting materials. The simple, fast, and environmentally friendly column-based procedure does not use phenol, chloroform, or acetone.

NucleoSpin RNA/Protein is a complete mini kit with recombinant DNase and shredders for rapid, high-yield isolation and purification of total RNA and protein from cells and tissue. This kit allows simultaneous isolation of RNA and protein from a single lysate in one working procedure, from a wide variety of starting materials. The simple, fast, and environmentally friendly column-based procedure does not use phenol, chloroform, or acetone.

This method is very useful for researchers interested in gene regulation mechanisms, such as knockdown of mRNA expression by siRNAs and its resulting influence on protein expression. Such studies are often complicated by small sample sizes and the need to use differing and often incompatible techniques to obtain RNA and protein. Dividing a sample into several parts for independent purification procedures is difficult, especially for small samples (e.g., biopsy material). Moreover, the isolation of RNA and protein from different sample aliquots usually necessitates proof of sample equality because the analysis might be mistrusted if the experimental samples used for nucleic acid and protein purification are not absolutely identical. NucleoSpin RNA/Protein resolves these issues by accommodating small sample sizes and providing compatible techniques for RNA and protein isolation.

Samples are lysed by incubation in a buffer containing large amounts of chaotropic salts. This not only destroys the cell structure of the sample, but simultaneously inactivates enzymes such as RNases, phosphatases, and proteases, and thus prevents RNAs and proteins from being degraded. The buffer dissolves even sparingly soluble proteins virtually quantitatively, and at the same time creates appropriate binding conditions for RNA adsorption to the silica membrane of RNA/Protein Columns. Thus, RNA and protein from the same sample can be separated by simply centrifuging the lysate through the NucleoSpin RNA/Protein spin column—the RNA is bound to the membrane while the protein is resolved in the column flowthrough.

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Cat. # Product Size Price License Quantity Details
740933.50 NucleoSpin® RNA/Protein 50 Preps $362.00

NucleoSpin RNA/Protein allows for the concurrent isolation of RNA and total cellular protein in separate fractions from undivided samples in one working procedure. The ability to isolate RNA and protein directly from the same sample without splitting minimizes the likelihood of experimental artifacts and is especially valuable for unique, small, and precious samples. Isolated RNA is suitable for all common downstream applications and is of identical high quality as RNA isolated with the well-proven NucleoSpin RNA kits. Isolated protein is immediately suitable for SDS-PAGE and Western blot analysis.

Cat. # 740933.50 includes sufficient quantities of reagents and materials for processing of 50 samples. 

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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NucleoSpin RNA/Protein procedure

NucleoSpin RNA/Protein procedure
NucleoSpin RNA/Protein procedure.
740933.250 NucleoSpin® RNA/Protein 250 Preps $1645.00

NucleoSpin RNA/Protein allows for the concurrent isolation of RNA and total cellular protein in separate fractions from undivided samples in one working procedure. The ability to isolate RNA and protein directly from the same sample without splitting minimizes the likelihood of experimental artifacts and is especially valuable for unique, small, and precious samples. Isolated RNA is suitable for all common downstream applications and is of identical high quality as RNA isolated with the well-proven NucleoSpin RNA kits. Isolated protein is immediately suitable for SDS-PAGE and Western blot analysis.

Cat. # 740933.250 includes sufficient quantities of reagents and materials for processing of 250 samples. 

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

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NucleoSpin RNA/Protein procedure

NucleoSpin RNA/Protein procedure
NucleoSpin RNA/Protein procedure.
740933.10 NucleoSpin® RNA/Protein 10 Preps $75.20

NucleoSpin RNA/Protein allows for the concurrent isolation of RNA and total cellular protein in separate fractions from undivided samples in one working procedure. The ability to isolate RNA and protein directly from the same sample without splitting minimizes the likelihood of experimental artifacts and is especially valuable for unique, small, and precious samples. Isolated RNA is suitable for all common downstream applications and is of identical high quality as RNA isolated with the well-proven NucleoSpin RNA kits. Isolated protein is immediately suitable for SDS-PAGE and Western blot analysis.

Cat. # 740933.10 includes sufficient quantities of reagents and materials for processing of 10 samples. 

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents

Efficient and quick RNA isolation from cells and tissues, including genomic DNA removal column NucleoSpin RNA Plus
Selection guides RNA purification kit selection guide
Overviews Parallel RNA/Protein purification

Overview

  • Complete mini kit with rDNase* and shredders
  • High-quality RNA suitable for downstream applications
  • High protein yield independent of protein size, localization, or modifications
  • Simplifies transcriptional and translational gene expression profiling
  • Easy protein quantification with the Protein Quantification Assay

* The amount of DNA contamination is significantly reduced during on-column rDNase digestion. However, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA/Protein system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1-kb fragment in a 30-cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with:

  • The number of DNA copies per preparation:
    Single copy target < plastidial/mitochondrial target < cells transfected with plasmid
  • Decreasing PCR amplicon size

More Information

Technology Silica membrane
Format Mini spin columns
Starting material <5 x 106 cultured cells
<30 mg human/animal tissue
100 mg plant tissue
Total RNA Total Protein
Fragment size 200 bp–20 kb 15–200 kDa
Typical yield <70 µg <1,200 µg
A260/280 1.90–2.10 Does not apply
Elution volume 40–100 µl 10–100 µl
Preparation time 30 min/6 preps 35 min/6 preps

Applications

  • Rapid isolation and purification of total RNA and protein from cultured cells and tissue
  • Gene expression profiling, siRNA experiments, analysis of transgenic organisms, and drug screening
  • Use for RT-PCR, 5' nuclease assays for probe-based qPCR, blotting, and microarrays, among others

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


Concurrent isolation of RNA and total cellular protein from a single sample

NucleoSpin RNA/Protein application data

This product overview describes the use of the NucleoSpin RNA/Protein kit for the concurrent isolation of RNA and total cellular protein from undivided samples in one working procedure. Protein and RNA are obtained in separate fractions. This kit is especially valuable for unique, small, and precious samples. Isolated RNA is suitable for all common downstream applications and is of comparable high quality to RNA isolated with the proven NucleoSpin RNA kit. Isolated protein is immediately suitable for SDS-PAGE and Western blot analysis.

Learn more Other RNA purification kits

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

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Speed up your mass spec workflow

Capturem Trypsin provides rapid, efficient, and complete digestion of protein samples, allowing an uninterrupted mass spectometry workflow at room temperature for downstream protein analysis. This product utilizes our novel Capturem technology in a spin column format with membrane-immobilized trypsin. Capturem Trypsin Columns may be used to completely digest protein samples in less than a minute with digestion efficiencies (protein coverage) comparable to or better than those obtained using in-solution trypsin digestion.

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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