NucleoBond RNA/DNA—Isolation & purification of RNA & DNA from the same sample
NucleoBond RNA/DNA 80 and 400 kits are designed for the isolation and purification of up to 80 or 400 µg of ultra-pure total RNA, respectively, plus a separate genomic DNA fraction. A special manufacturing process guarantees RNase-free handling. These kits contain NucleoBond AXR columns (anion-exchange columns for the highest purity and yield) and all of the required buffers. Due to the resolving power of NucleoBond anion-exchange resin, contaminants are washed away, and RNA and genomic DNA can be eluted separately.
Overview
- Extra-high purity for up to 400 µg of total RNA via anion-exchange chromatography
- Separate isolation of different RNA species (tRNA, rRNA, mRNA)
- Separate elution of genomic DNA
More Information
NucleoBond RNA/DNA 80 |
NucleoBond RNA/DNA 400 |
|
Technology |
Anion-exchange chromatography |
Anion-exchange chromatography |
Format |
Midi gravity-flow columns |
Maxi gravity-flow columns |
Starting material |
5 x 106 cultured eukaryotic cells |
2 x 107 cultured eukaryotic cells |
Fragment size |
50 bp–300 kb |
50 bp–300 kb |
Typical yield |
70 µg from 5 x 106 cultured cells |
300 µg from 2 x 107 cultured cells |
A260/280 |
1.80–1.95 |
1.80–1.95 |
Preparation time |
1.5–2.5 hr |
1.5–2.5 hr |
Binding capacity |
80 µg |
400 µg |
Applications
- Isolate and purify total RNA and genomic DNA from cultured cells, tissue, and bacteria.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Designed for extra small samples
Purify DNA from small samples such as microdissected tissue, small blood samples, or small amounts of cells. Based on reliable silica-membrane technology, NucleoSpin Tissue XS overcomes the limitations of conventional kits using an innovative new column design with a funnel-shaped ring.
Learn more All genomic DNA kitsTakara Bio USA, Inc.
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