High-throughput total RNA isolation from blood—NucleoSpin 8/96 RNA Blood
The NucleoSpin 8/96 RNA Blood kits combine the stringency of guanidine-isothiocyanate lysis with effective digestion of genomic DNA by rDNase treatment and the speed of silica membrane technology, allowing purification of total RNA from up to 400 µl of whole blood. These kits offer consistent RNA quality, reproducible yields of RNA, and high-throughput procedures free of cross-contamination.
The NucleoSpin 8/96 RNA Blood kits combine the stringency of guanidine-isothiocyanate lysis with effective digestion of genomic DNA by rDNase treatment and the speed of silica membrane technology, allowing purification of total RNA from up to 400 µl of whole blood. These kits offer consistent RNA quality, reproducible yields of RNA, and high-throughput procedures free of cross-contamination.
In the NucleoSpin 8/96 RNA Blood procedure, blood cells are directly lysed by incubation in a solution containing large amounts of chaotropic ions. This lysis buffer immediately inactivates RNases and creates appropriate binding conditions which favor adsorption of RNA to the silica membrane of the provided binding strips/plates. Contaminating DNA, which is also bound to the silica membrane, is efficiently removed by on-column DNA digestion (a 15-min digestion at room temperature is sufficient, due to the highly specific activity of the rDNase). Salts, metabolites, and macromolecular cellular components are removed by simple washing steps with three different buffers. Pure RNA is eluted under low ionic strength conditions with RNase-free water.
The NucleoSpin 8/96 RNA Blood kits provide a powerful tool for diverse research applications, including gene expression profiling, drug discovery, and development of novel molecular diagnostics.
Overview
- Simple, direct total blood lysis at room temperature
- Compatible with common blood collection tubes and anticoagulants (e.g., EDTA, citrate, or heparin)
- Suitable for fresh and frozen blood samples
- rDNase included for efficient removal of genomic DNA
- Suitable for manual and automated processing, under vacuum or centrifugation
- Innovative MN Wash Plate minimizes risk of cross-contamination
More Information
| NucleoSpin 8 RNA Blood | NucleoSpin 96 RNA Blood | |
| Technology | Silica membrane | |
| Format | 8-well strips | 96-well plates |
| Processing | Manual or automated, vacuum or centrifugation | |
| Starting material | <400 µl whole blood (fresh or frozen) | |
| Fragment size | >200 nt | |
| Typical yield | 1–8 µg (400 µl blood)* | |
| Typical RNA quality (A260/A280) | 1.9–2.1 | |
| Elution volume | 50–130 µl | |
| Preparation time | 60 min/6 strips | 100 min/plate |
| Binding capacity | 100 µg | |
*RNA yield strongly depends on the leukocyte number in each individual blood sample.
Applications
- Manual or automated isolation of total RNA from fresh or frozen whole blood stabilized with anticoagulants (e.g., EDTA, citrate, or heparin)
- Typical downstream applications: qRT-PCR, next-generation sequencing, blotting, array technology
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2024 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.
