SMARTScribe Reverse Transcriptase

SMARTScribe Reverse Transcriptase is a high-performance enzyme that performs unbiased cDNA synthesis, allowing for amplification and library construction from any RNA transcript. SMARTScribe RT is a modified Moloney Murine Leukemia Virus reverse transcriptase that generates long, full-length cDNA (up to 14.7 kb) while preserving the relative transcript proportions of the original RNA sample. Our proprietary purification process and rigorous quality control standards ensure that virtually all contaminating nucleases have been removed from SMARTScribe RT. It has been specially formulated for use with all of our SMART kits.

Cat. #	Product	Size	Price
639538	SMARTScribe™ Reverse Transcriptase	400 Rxns	USD $815.00
SMARTScribe Reverse Transcriptase is a modified Moloney Murine Leukemia Virus Reverse Transcriptase that synthesizes full-length cDNA from rare or long transcripts. SMARTScribe RT preparations are exceptionally pure, with all contaminating nucleases removed. The enzyme is useful for a wide range of applications, and has been specially formulated for use with all of our SMART Kits. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back SMARTScribe RT exhibits exceptional sensitivity SMARTScribe RT exhibits exceptional sensitivity. RT-PCR assays were performed using SMARTScribe RT and synthetic RNA. The synthetic RNA was serially diluted 10X to obtain 105–110 copies per sample; diluted template was then spiked into 50 ng of total RNA from HeLa cells to increase complexity. First-strand cDNA was synthesized with SMARTScribe RT, then amplified with Advantage® 2 DNA Polymerase Mix in 36 cycles of PCR using primers specific for a 350-bp amplicon. The PCR reactions were then analyzed by agarose gel electrophoresis. SMARTScribe was able to generate single-stranded cDNA from as few as 10 copies of synthetic RNA. Lane M: 100 bp DNA size marker. Back SMARTScribe RT is more sensitive than the competition SMARTScribe RT is more sensitive than the competition. Human Universal Total RNA was serially diluted 10X (from 1 μg to 0.1 pg), then reverse transcribed using either SMARTScribe RT (Panel A), Competitor P (Panel B) or Competitor Q (Panel C). Each RT reaction was performed according to the enzyme manufacturer’s specifications. One microliter of each RT reaction was then used in PCR reactions with primers specific for a 300-bp portion of the 5' end of the beta-actin gene. The samples were analyzed by agarose gel electrophoresis. The amount of template RNA used in each reaction is as follows: Lane 1: 1 μg. Lane 2: 100 ng. Lane 3: 10 ng. Lane 4: 1 ng. Lane 5: 100 pg. Lane 6: 10 pg. Lane 7: 1 pg. Lane 8: 0.1 pg. SMARTScribe RT exhibited superior sensitivity at all of the template concentrations tested. Back Comparison of SMARTScribe RT and SMART MMLV RT Comparison of SMARTScribe RT and SMART MMLV RT. Back SMARTScribe Reverse Transcriptase
639537	SMARTScribe™ Reverse Transcriptase	100 Rxns	USD $227.00
SMARTScribe Reverse Transcriptase is a modified Moloney Murine Leukemia Virus Reverse Transcriptase that synthesizes full-length cDNA from rare or long transcripts. SMARTScribe RT preparations are exceptionally pure, with all contaminating nucleases removed. The enzyme is useful for a wide range of applications, and has been specially formulated for use with all of our SMART Kits. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back SMARTScribe RT exhibits exceptional sensitivity SMARTScribe RT exhibits exceptional sensitivity. RT-PCR assays were performed using SMARTScribe RT and synthetic RNA. The synthetic RNA was serially diluted 10X to obtain 105–110 copies per sample; diluted template was then spiked into 50 ng of total RNA from HeLa cells to increase complexity. First-strand cDNA was synthesized with SMARTScribe RT, then amplified with Advantage® 2 DNA Polymerase Mix in 36 cycles of PCR using primers specific for a 350-bp amplicon. The PCR reactions were then analyzed by agarose gel electrophoresis. SMARTScribe was able to generate single-stranded cDNA from as few as 10 copies of synthetic RNA. Lane M: 100 bp DNA size marker. Back SMARTScribe RT is more sensitive than the competition SMARTScribe RT is more sensitive than the competition. Human Universal Total RNA was serially diluted 10X (from 1 μg to 0.1 pg), then reverse transcribed using either SMARTScribe RT (Panel A), Competitor P (Panel B) or Competitor Q (Panel C). Each RT reaction was performed according to the enzyme manufacturer’s specifications. One microliter of each RT reaction was then used in PCR reactions with primers specific for a 300-bp portion of the 5' end of the beta-actin gene. The samples were analyzed by agarose gel electrophoresis. The amount of template RNA used in each reaction is as follows: Lane 1: 1 μg. Lane 2: 100 ng. Lane 3: 10 ng. Lane 4: 1 ng. Lane 5: 100 pg. Lane 6: 10 pg. Lane 7: 1 pg. Lane 8: 0.1 pg. SMARTScribe RT exhibited superior sensitivity at all of the template concentrations tested. Back Comparison of SMARTScribe RT and SMART MMLV RT Comparison of SMARTScribe RT and SMART MMLV RT. Back 639537: SMARTScribe Reverse Transcriptase
639536	SMARTScribe™ Reverse Transcriptase	40 Rxns	USD $105.00
SMARTScribe Reverse Transcriptase is a modified Moloney Murine Leukemia Virus Reverse Transcriptase that synthesizes full-length cDNA from rare or long transcripts. SMARTScribe RT preparations are exceptionally pure, with all contaminating nucleases removed. The enzyme is useful for a wide range of applications, and has been specially formulated for use with all of our SMART Kits. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 639536: SMARTScribe Reverse Transcriptase Back SMARTScribe RT exhibits exceptional sensitivity SMARTScribe RT exhibits exceptional sensitivity. RT-PCR assays were performed using SMARTScribe RT and synthetic RNA. The synthetic RNA was serially diluted 10X to obtain 105–110 copies per sample; diluted template was then spiked into 50 ng of total RNA from HeLa cells to increase complexity. First-strand cDNA was synthesized with SMARTScribe RT, then amplified with Advantage® 2 DNA Polymerase Mix in 36 cycles of PCR using primers specific for a 350-bp amplicon. The PCR reactions were then analyzed by agarose gel electrophoresis. SMARTScribe was able to generate single-stranded cDNA from as few as 10 copies of synthetic RNA. Lane M: 100 bp DNA size marker. Back SMARTScribe RT is more sensitive than the competition SMARTScribe RT is more sensitive than the competition. Human Universal Total RNA was serially diluted 10X (from 1 μg to 0.1 pg), then reverse transcribed using either SMARTScribe RT (Panel A), Competitor P (Panel B) or Competitor Q (Panel C). Each RT reaction was performed according to the enzyme manufacturer’s specifications. One microliter of each RT reaction was then used in PCR reactions with primers specific for a 300-bp portion of the 5' end of the beta-actin gene. The samples were analyzed by agarose gel electrophoresis. The amount of template RNA used in each reaction is as follows: Lane 1: 1 μg. Lane 2: 100 ng. Lane 3: 10 ng. Lane 4: 1 ng. Lane 5: 100 pg. Lane 6: 10 pg. Lane 7: 1 pg. Lane 8: 0.1 pg. SMARTScribe RT exhibited superior sensitivity at all of the template concentrations tested. Back Comparison of SMARTScribe RT and SMART MMLV RT Comparison of SMARTScribe RT and SMART MMLV RT.

Overview

Synthesize long, full-length cDNA up to 14.7 kb
Amplify rare transcripts
Maintain the complexity of the original RNA
Assay the 5' end of transcripts

More Information

Applications

RT-PCR
Real-time PCR/qPCR
Gene expression analysis
Cloning
Library construction
Microarray

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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SMARTScribe Reverse Transcriptase

Notice to purchaser

SMARTScribe RT exhibits exceptional sensitivity

SMARTScribe RT is more sensitive than the competition

Comparison of SMARTScribe RT and SMART MMLV RT