DNA Fragmentation Kit
The DNA Fragmentation Kit is designed to perform random genomic DNA fragmentation and other long-chain dsDNA fragmentation via enzymatic treatment followed by blunting of the resulting DNA fragments. The kit does not require an additional physical shearing step using an apparatus such as a sonicator. Blunt-end DNA fragments may be ligated with blunt-end vectors. If blunting is not required, the reaction may be stopped after DNA fragmentation is complete. The kit may also be used to pretreat samples enriched with methylated DNA and to prepare DNA samples for sequencing.
More Information
Applications
- Genomic DNA fragmentation
- dsDNA fragmentation
Components
| 20 preparations | |
|---|---|
| Enzyme-1 | 20 µl |
| Dilution Buffer-1 | 1,040 µl |
| A Solution | 20 µl |
| B Solution | 50 µl |
| Stop Solution | 400 µl |
| 150 mM MgCl2 | 40 µl |
| Dilution Buffer-2 | 200 µl |
| Enzyme-2 | 20 µl |
| 0.5 M EDTA | 50 µl |
| dH2O | 10 × 1 ml |
Storage
–20°C. Solution A, Solution B, 150 mM MgCl2, 0.5 M EDTA and dH2O may be stored at 4°C.
Materials required but not provided
- Thermal cycler (at least 1 cycler is required; 2 cyclers are preferred)
- Electrophoresis loading buffer
We recommend using a loading buffer that contains a dye (e.g., Orange G) that does not overlap with dsDNA fragmentation bands ranging from 100 bp to 1,000 bp in size on the electrophoresis gel. When using BPB or xylene cyanol, please be aware that these dyes overlap with dsDNA fragments in the 100 bp to 1,000 bp size range.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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