AMV Reverse Transcriptase XL
AMV Reverse Transcriptase XL synthesizes complementary full-length cDNA from single-stranded RNA, DNA, or RNA-DNA hybrid templates. The enzyme requires a primer to initiate synthesis and Mg2+ or Mn2+ for activity. AMV Reverse Transcriptase XL is positive for 5→3' ribonuclease H activity and can be used for a number of applications, including reverse transcription. AMV Reverse Transcriptase XL is highly purified, free of extraneous nucleases and is qualified for use in full-length cDNA synthesis and reverse transcription using RNA templates that are up to 10 kb in length. It can be used over a temperature range of 37–55°C, but maximal yield of full-length cDNA products is achieved between 41–44°C.
- Reverse transcription
- Full-length cDNA synthesis
- Primer extension
- Preparation of DNA probes
Avian Myeloblastosis Virus (Manufactured by Life Science Co.).
–80°C. Avoid repeated freeze-thaw. Can be stored at –20° for up to 6 months.
One unit is defined as the amount of AMV RT XL, required to catalyze the incorporation of one nmol of [3H] dTMP into acid-insoluble products in 10 minutes at 37°C using poly (rA) • oligo(dT)12–18 as the template-primer.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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