- cDNA synthesis kits
- Reverse transcriptases
- RACE kits
- Purified cDNA & genomic DNA
- Purified total RNA and mRNA
- cDNA synthesis accessories
The One-Step RNA PCR Kit (AMV) offers the convenience and time savings of performing reverse transcription and PCR in a single tube via one-step RT-PCR. This kit thus eliminates the need to change buffers or add reagents and minimizes the risk of contamination. The AMV Reverse Transcriptase XL used for cDNA synthesis is more stable than other reverse transcriptases, allowing reactions to retain activity over a wide range of temperatures. The kit also contains AMV-Optimized Taq DNA Polymerase for efficient amplification of cDNA synthesized via AMV Reverse Transcriptase XL.
- One-step RT-PCR
- cDNA synthesis
|AMV Reverse Transcriptase XL* (5 U/µl)||50 µl|
|AMV-Optimized Taq DNA Polymerase (5 U/µl)||50 µl|
|RNase Inhibitor (40 U/µl)||60 µl|
|RNase-Free dH2O (DEPC-treated)||2 x 1 ml|
|10X One-Step RNA PCR Buffer||250 µl|
|dNTP Mixture (10 mM each)||250 µl|
|MgCl2 (25 mM)||500 µl|
|Control F-1 Primer (20 pmol/µl)||25 µl|
|Control R-1 Primer (20 pmol/µl)||25 µl|
|Positive control RNA (2 x 105 copies/µl)||25 µl|
*Manufactured by Life Science Co.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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