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In-Fusion Cloning guide
In-Fusion PCR Cloning systems enable directional, seamless cloning of any PCR fragment—or multiple fragments—into any linearized vector with high accuracy and high fidelity. No additional treatment of the PCR fragment—such as restriction digestion, ligation, phosphorylation, or blunt-end polishing—is needed. The efficiency of In-Fusion HD Cloning kits is over 95%, and just one quick reaction allows you to recover your final construct. This versatile system easily outpaces any traditional cloning method, including ligation and TA cloning.
Which kit best fits my needs?
|Features||In-Fusion HD Cloning Plus System||In-Fusion HD EcoDry Cloning Plus System|
|Ability to customize reaction volume and/or plasticware|
|Easily scalable for high-throughput applications|
|Minimize handling with prealiquoted, lyophilized components|
|Store reagents at room temperature|
|Compatible with Cloning Enhancer for fast, enzymatic removal of background DNA and PCR residue when PCR produces a single, clean band|
|Compatible with PCR purification and gel extraction kits (such as NucleoSpin Gel and PCR Clean-up kits) when PCR produces >1 product|
All In-Fusion HD Cloning Plus kits contain CloneAmp HiFi PCR Premix, In-Fusion HD Enzyme Premix, and reagents for control experiments (linearized vector and a 2-kb control insert).
|Save time||Be efficient||Don’t compromise on flexibility|
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