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  • ‹ Back to In-Fusion Cloning general information
  • In-Fusion Cloning overview
  • In-Fusion Cloning guide
  • In-Fusion Cloning and competition
  • In-Fusion Cloning citations
  • Stellar Competent Cells product overview and performance data
  • EcoDry reagents and sustainability
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Home › Learning centers › Cloning › In-Fusion Cloning general information › In-Fusion Cloning overview

Cloning

  • Automated In-Fusion Cloning
  • In-Fusion Cloning general information
    • In-Fusion Cloning overview
    • In-Fusion Cloning guide
    • In-Fusion Cloning and competition
      • In-Fusion Snap Assembly vs. GeneArt Gibson Assembly HiFi
      • In-Fusion Snap Assembly vs. NEBuilder HiFi
      • Sequence accuracy in seamless cloning
      • Choosing a seamless cloning method
      • Improving background over Gibson Assembly
      • A successful alternative to ligation cloning
      • Single- and multiple-insert cloning
      • Easy cloning into lentiviral vectors
      • Outperforming TOPO cloning
    • In-Fusion Cloning citations
    • Stellar Competent Cells product overview and performance data
    • EcoDry reagents and sustainability
  • Primer design and other tools
    • Seamless cloning primer design
    • In-Fusion Cloning tutorials
      • Cloning one or more fragments
      • Deleting a sequence
      • Inserting a sequence
      • Deleting and replacing a sequence
    • In-Fusion molar ratio calculator
    • Simulate your construct
  • In‑Fusion Cloning tips and FAQs
  • Applications and technical notes
    • In-Fusion Cloning applications collection
    • Efficient multiple-fragment cloning
    • Mutagenesis with In-Fusion Cloning
    • Rapid, high-throughput cloning for antibody development
    • Solve a synthesis challenge with easy multiple-insert cloning
    • Direct cloning into large vectors
    • Simplified insertion of a GFP-encoding cassette into a 100-kb plasmid
    • Efficient cloning for sgRNA/Cas9 plasmids
    • In-Fusion Cloning of sgRNAs
    • De novo insertion of small fusion protein tags
  • Sign up to stay updated
  • Traditional molecular cloning
    • Restriction enzyme overview
      • General information about restriction enzymes
      • Star activity of restriction enzymes
      • Inactivation of restriction enzymes
      • Buffer activity with restriction enzymes
      • Universal buffers for double digestion with restriction enzymes
      • Restriction enzymes affected by methylation
      • Methylation-sensitive restriction enzymes
      • QC of restriction enzymes
    • Ligation cloning overview
    • Ligation product guide
  • In-Fusion Cloning webinars
New products
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Overview

In-Fusion Cloning

  • Exceptional accuracy minimizes screening and saves time
  • Unique mechanism prevents junction errors and empty vector background
  • Unparalleled versatility for a variety of applications
  • Quick, scalable workflow that can be easily automated
The right clone every time One product, multiple applications A beautifully simple workflow

The right clone every time  

In-Fusion Cloning kits are unique in their ability to allow easy and accurate seamless cloning without the use of ligase. In just one quick reaction, you can clone any insert into any vector at any locus, without the hassle of subcloning, unwanted extra bases, or inconvenient restriction sites. Watch our videos to learn more »

streamlined seamless cloning workflow

Figure 1. In-Fusion Cloning protocol. The key to In-Fusion Cloning is 15 bp of homology between your insert(s) and linearized vector backbone.

One product, multiple applications  

In-Fusion Cloning is beautifully versatile. With an easy-to-follow protocol that provides high efficiency, you can successfully perform a wide array of applications, all with the same kit. Clone your insert specifically and directionally, and get an exceptionally high rate of cloning accuracy in every experiment.

Single-insert cloning

cloning a single insert

Streamlined multi-fragment cloning

Site-directed mutagenesis

High-throughput cloning

Cloning of antibody variable regions

Synthetic gene assembly

Large-insert cloning

Direct cloning into large vectors

Lentiviral cloning

A beautifully simple workflow  

The homologous ends between insert(s) and vector are designed into your primers, and the two are annealed during the In-Fusion Cloning reaction. Because there is no need to digest the insert(s) prior to incubation with the vector, the In-Fusion Cloning workflow is significantly shorter than that of traditional cloning by restriction enzymes. This technology does not require a ligation step either, saving even more time and getting you to the transformation step sooner. With empty vector background levels approaching zero, you can pick just a few colonies and be confident that you have the right clone. Cloning shouldn't be work; it should just work!

In-Fusion workflow comparison

Figure 2. In-Fusion Cloning and traditional cloning workflows. The In-Fusion Cloning protocol is simple and elegant. Just four simple steps guarantee cloning accuracy above 95%.

In-Fusion Cloning kits and sizes

Cat. # Product Size Price License Quantity Details
638943 In-Fusion® Snap Assembly Master Mix 500 Rxns Inquire for Quotation *

In-Fusion Snap Assembly Master Mix is designed for fast, directional cloning of one or more fragments of DNA into any vector. This proprietary master mix fuses DNA fragments (e.g., PCR-generated sequences and linearized vectors) efficiently and precisely by recognizing a 15-bp overlap at their ends. This 15-bp overlap can be engineered into the primers designed for PCR amplification of the desired sequences. In Fusion Snap Assembly Master Mix offers high efficiency, even for applications that can be challenging, including the cloning of long fragments, short oligonucleotides, and multiple fragments.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

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638943: In-Fusion Snap Assembly Master Mix

638943: In-Fusion Snap Assembly Master Mix

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Performance comparison between In-Fusion Snap Assembly and NEBuilder HiFi using inverse PCR.

Performance comparison between In-Fusion Snap Assembly and NEBuilder HiFi using inverse PCR.

Performance comparison between In-Fusion Snap Assembly and NEBuilder HiFi using inverse PCR. A single 3.8-kb insert (Panel A) or a 34.2-kb adenovirus insert (Panel B) was cloned into a 2.7-kb vector which was linearized via inverse PCR. These cloning reactions were performed in triplicate with both In-Fusion Snap Assembly and NEBuilder HiFi. Primers were designed according to the manufacturers' specifications. After transformation and plating, 20 colonies from each replicate were analyzed by Sanger sequencing (for the 3.8-kb insert) or colony PCR (for the adenovirus insert) to determine the cloning accuracy. In-Fusion Snap Assembly yielded 2X more colonies than NEBuilder HiFi.

Back

The In-Fusion cloning protocol

The In-Fusion cloning protocol

The In-Fusion cloning protocol.

638944 In-Fusion® Snap Assembly Master Mix 1,000 Rxns Inquire for Quotation *

In-Fusion Snap Assembly Master Mix is designed for fast, directional cloning of one or more fragments of DNA into any vector. This proprietary master mix fuses DNA fragments (e.g., PCR-generated sequences and linearized vectors) efficiently and precisely by recognizing a 15-bp overlap at their ends. This 15-bp overlap can be engineered into the primers designed for PCR amplification of the desired sequences. In Fusion Snap Assembly Master Mix offers high efficiency, even for applications that can be challenging, including the cloning of long fragments, short oligonucleotides, and multiple fragments.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

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Performance comparison between In-Fusion Snap Assembly and NEBuilder HiFi using inverse PCR.

Performance comparison between In-Fusion Snap Assembly and NEBuilder HiFi using inverse PCR.

Performance comparison between In-Fusion Snap Assembly and NEBuilder HiFi using inverse PCR. A single 3.8-kb insert (Panel A) or a 34.2-kb adenovirus insert (Panel B) was cloned into a 2.7-kb vector which was linearized via inverse PCR. These cloning reactions were performed in triplicate with both In-Fusion Snap Assembly and NEBuilder HiFi. Primers were designed according to the manufacturers' specifications. After transformation and plating, 20 colonies from each replicate were analyzed by Sanger sequencing (for the 3.8-kb insert) or colony PCR (for the adenovirus insert) to determine the cloning accuracy. In-Fusion Snap Assembly yielded 2X more colonies than NEBuilder HiFi.

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638944: In-Fusion Snap Assembly Master Mix

638944: In-Fusion Snap Assembly Master Mix

Back

The In-Fusion cloning protocol

The In-Fusion cloning protocol

The In-Fusion cloning protocol.

638945 In-Fusion® Snap Assembly Starter Bundle 10 Rxns USD $312.00

License Statement

ID Number  
M54 This product is covered by the claims of U.S. Patent Nos. 7,704,713 and its foreign counterparts. 

In-Fusion Snap Assembly Master Mix enables high-efficiency, high-fidelity, directional cloning of one or more PCR fragments into any vector. In addition to the cloning kit, this package includes:

- A NucleoSpin Gel and PCR Clean-Up kit: This kit is suitable for gel extraction as well as PCR purification. Kits are provided with individual purification columns.

- Stellar Competent Cells: High-efficiency competent cells are essential to the success of In-Fusion Cloning. An E. coli HST08 strain is included that provides high transformation efficiency (greater than 5 x 10^8 cfu/µg) and complements the efficiency of all In-Fusion Snap Assembly kits. Cells are provided in 100-μl aliquots in individual tubes.

- PrimeSTAR Max DNA Polymerase: This convenient 2X liquid master mix offers exceptionally accurate, efficient, and fast DNA amplification. The premix contains dNTPs and an optimized buffer, allows rapid setup of PCR reactions, and facilitates successful cloning. The polymerase master mix is provided in 625-μl aliquots.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Performance comparison between In-Fusion Snap Assembly and NEBuilder HiFi using inverse PCR.

Performance comparison between In-Fusion Snap Assembly and NEBuilder HiFi using inverse PCR.

Performance comparison between In-Fusion Snap Assembly and NEBuilder HiFi using inverse PCR. A single 3.8-kb insert (Panel A) or a 34.2-kb adenovirus insert (Panel B) was cloned into a 2.7-kb vector which was linearized via inverse PCR. These cloning reactions were performed in triplicate with both In-Fusion Snap Assembly and NEBuilder HiFi. Primers were designed according to the manufacturers' specifications. After transformation and plating, 20 colonies from each replicate were analyzed by Sanger sequencing (for the 3.8-kb insert) or colony PCR (for the adenovirus insert) to determine the cloning accuracy. In-Fusion Snap Assembly yielded 2X more colonies than NEBuilder HiFi.

Back

The In-Fusion cloning protocol

The In-Fusion cloning protocol

The In-Fusion cloning protocol.

Back

638945: In-Fusion Snap Assembly Starter Bundle

638945: In-Fusion Snap Assembly Starter Bundle
638946 In-Fusion® Snap Assembly Value Bundle 50 Rxns USD $1157.00

License Statement

ID Number  
M54 This product is covered by the claims of U.S. Patent Nos. 7,704,713 and its foreign counterparts. 

In-Fusion Snap Assembly Master Mix enables high-efficiency, high-fidelity, directional cloning of one or more PCR fragments into any vector. In addition to the cloning kit, this package includes:

- A NucleoSpin Gel and PCR Clean-Up kit: This kit is suitable for gel extraction as well as PCR purification. Kits are provided with individual purification columns.

- Stellar Competent Cells: High-efficiency competent cells are essential to the success of In-Fusion Cloning. An E. coli HST08 strain is included that provides high transformation efficiency (greater than 5 x 10^8 cfu/µg) and complements the efficiency of all In-Fusion Snap Assembly kits. Cells are provided in 100-μl aliquots in individual tubes.

- PrimeSTAR Max DNA Polymerase: This convenient 2X liquid master mix offers exceptionally accurate, efficient, and fast DNA amplification. The premix contains dNTPs and an optimized buffer, allows rapid setup of PCR reactions, and facilitates successful cloning. The polymerase master mix is provided in 625-μl aliquots.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

638946: In-Fusion Snap Assembly Value Bundle

638946: In-Fusion Snap Assembly Value Bundle

Back

Performance comparison between In-Fusion Snap Assembly and NEBuilder HiFi using inverse PCR.

Performance comparison between In-Fusion Snap Assembly and NEBuilder HiFi using inverse PCR.

Performance comparison between In-Fusion Snap Assembly and NEBuilder HiFi using inverse PCR. A single 3.8-kb insert (Panel A) or a 34.2-kb adenovirus insert (Panel B) was cloned into a 2.7-kb vector which was linearized via inverse PCR. These cloning reactions were performed in triplicate with both In-Fusion Snap Assembly and NEBuilder HiFi. Primers were designed according to the manufacturers' specifications. After transformation and plating, 20 colonies from each replicate were analyzed by Sanger sequencing (for the 3.8-kb insert) or colony PCR (for the adenovirus insert) to determine the cloning accuracy. In-Fusion Snap Assembly yielded 2X more colonies than NEBuilder HiFi.

Back

The In-Fusion cloning protocol

The In-Fusion cloning protocol

The In-Fusion cloning protocol.

638948 In-Fusion® Snap Assembly Master Mix 50 Rxns USD $750.00

In-Fusion Snap Assembly Master Mix is designed for fast, directional cloning of one or more fragments of DNA into any vector. This proprietary master mix fuses DNA fragments (e.g., PCR-generated sequences and linearized vectors) efficiently and precisely by recognizing a 15-bp overlap at their ends. This 15-bp overlap can be engineered into the primers designed for PCR amplification of the desired sequences. In Fusion Snap Assembly Master Mix offers high efficiency, even for applications that can be challenging, including the cloning of long fragments, short oligonucleotides, and multiple fragments.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Back

638948: In-Fusion Snap Assembly Master Mix

638948: In-Fusion Snap Assembly Master Mix

Back

Performance comparison between In-Fusion Snap Assembly and NEBuilder HiFi using inverse PCR.

Performance comparison between In-Fusion Snap Assembly and NEBuilder HiFi using inverse PCR.

Performance comparison between In-Fusion Snap Assembly and NEBuilder HiFi using inverse PCR. A single 3.8-kb insert (Panel A) or a 34.2-kb adenovirus insert (Panel B) was cloned into a 2.7-kb vector which was linearized via inverse PCR. These cloning reactions were performed in triplicate with both In-Fusion Snap Assembly and NEBuilder HiFi. Primers were designed according to the manufacturers' specifications. After transformation and plating, 20 colonies from each replicate were analyzed by Sanger sequencing (for the 3.8-kb insert) or colony PCR (for the adenovirus insert) to determine the cloning accuracy. In-Fusion Snap Assembly yielded 2X more colonies than NEBuilder HiFi.

Back

The In-Fusion cloning protocol

The In-Fusion cloning protocol

The In-Fusion cloning protocol.

638949 In-Fusion® Snap Assembly Master Mix 250 Rxns USD $2979.00

In-Fusion Snap Assembly Master Mix is designed for fast, directional cloning of one or more fragments of DNA into any vector. This proprietary master mix fuses DNA fragments (e.g., PCR-generated sequences and linearized vectors) efficiently and precisely by recognizing a 15-bp overlap at their ends. This 15-bp overlap can be engineered into the primers designed for PCR amplification of the desired sequences. In Fusion Snap Assembly Master Mix offers high efficiency, even for applications that can be challenging, including the cloning of long fragments, short oligonucleotides, and multiple fragments.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Back

Performance comparison between In-Fusion Snap Assembly and NEBuilder HiFi using inverse PCR.

Performance comparison between In-Fusion Snap Assembly and NEBuilder HiFi using inverse PCR.

Performance comparison between In-Fusion Snap Assembly and NEBuilder HiFi using inverse PCR. A single 3.8-kb insert (Panel A) or a 34.2-kb adenovirus insert (Panel B) was cloned into a 2.7-kb vector which was linearized via inverse PCR. These cloning reactions were performed in triplicate with both In-Fusion Snap Assembly and NEBuilder HiFi. Primers were designed according to the manufacturers' specifications. After transformation and plating, 20 colonies from each replicate were analyzed by Sanger sequencing (for the 3.8-kb insert) or colony PCR (for the adenovirus insert) to determine the cloning accuracy. In-Fusion Snap Assembly yielded 2X more colonies than NEBuilder HiFi.

Back

638949: In-Fusion Snap Assembly Master Mix

638949: In-Fusion Snap Assembly Master Mix

Back

The In-Fusion cloning protocol

The In-Fusion cloning protocol

The In-Fusion cloning protocol.

638951 In-Fusion® Snap Assembly Master Mix with Competent Cells 10 Rxns USD $258.00

In-Fusion Snap Assembly Master Mix with Competent Cells enables high-efficiency, high-fidelity, directional cloning of one or more PCR fragments into any vector. The In-Fusion Snap Assembly Master Mix offers high cloning efficiency, especially for longer fragments, short oligonucleotides, and multiple fragments. In addition to the cloning kit, this package includes Stellar™ Competent Cells, an E. coli HST08 strain that provides high transformation efficiency paired with blue-white screening capability when used with pUC plasmid vectors.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

The In-Fusion cloning protocol

The In-Fusion cloning protocol

The In-Fusion cloning protocol.

638952 In-Fusion® Snap Assembly Master Mix with Competent Cells 50 Rxns USD $987.00

In-Fusion Snap Assembly Master Mix with Competent Cells enables high-efficiency, high-fidelity, directional cloning of one or more PCR fragments into any vector. The In-Fusion Snap Assembly Master Mix offers high cloning efficiency, especially for longer fragments, short oligonucleotides, and multiple fragments. In addition to the cloning kit, this package includes Stellar™ Competent Cells, an E. coli HST08 strain that provides high transformation efficiency paired with blue-white screening capability when used with pUC plasmid vectors.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

The In-Fusion cloning protocol

The In-Fusion cloning protocol

The In-Fusion cloning protocol.

638953 In-Fusion® Snap Assembly EcoDry™ Master Mix with Competent Cells 96 Rxns USD $1633.00

The In-Fusion Snap Assembly EcoDry Master Mix with Competent Cells enables high-efficiency, high-fidelity, directional cloning of one or more PCR fragments into any vector. The cornerstone of In-Fusion Cloning technology is Takara Bio’s proprietary In-Fusion master mix, which fuses DNA fragments (e.g., PCR-generated sequences and linearized vectors) efficiently and precisely by recognizing a 15-bp overlap at their ends. This 15-bp overlap can be engineered by designing primers for amplification of the desired sequences. The In-Fusion Snap Assembly Master Mix offers high cloning efficiency, especially for longer fragments, short oligonucleotides, and multiple fragments. It is provided in a convenient, lyophilized (dry) format. In addition to the cloning kit, this package includes Stellar™ Competent Cells, an E. coli HST08 strain that provides high transformation efficiency paired with blue-white screening capability when used with pUC plasmid vectors.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Back

The In-Fusion cloning protocol

The In-Fusion cloning protocol

The In-Fusion cloning protocol.

Back

638953: In-Fusion Snap Assembly EcoDry Master Mix with Competent Cells

638953: In-Fusion Snap Assembly EcoDry Master Mix with Competent Cells
638954 In-Fusion® Snap Assembly EcoDry™ Master Mix 8 Rxns USD $190.00

The In-Fusion Snap Assembly EcoDry Master Mix enables high-efficiency, high-fidelity, directional cloning of one or more PCR fragments into any vector. The cornerstone of In-Fusion Cloning technology is Takara Bio’s proprietary In-Fusion master mix, which fuses DNA fragments (e.g., PCR-generated sequences and linearized vectors) efficiently and precisely by recognizing a 15-bp overlap at their ends. This 15-bp overlap can be engineered by designing primers for amplification of the desired sequences. The In-Fusion Snap Assembly Master Mix offers high cloning efficiency, especially for longer fragments, short oligonucleotides, and multiple fragments. It is provided in a convenient, lyophilized (dry) format.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Back

638954: In-Fusion Snap Assembly EcoDry Master Mix

638954: In-Fusion Snap Assembly EcoDry Master Mix

Back

The In-Fusion cloning protocol

The In-Fusion cloning protocol

The In-Fusion cloning protocol.

638955 In-Fusion® Snap Assembly EcoDry™ Master Mix 32 Rxns USD $568.00

The In-Fusion Snap Assembly EcoDry Master Mix enables high-efficiency, high-fidelity, directional cloning of one or more PCR fragments into any vector. The cornerstone of In-Fusion Cloning technology is Takara Bio’s proprietary In-Fusion master mix, which fuses DNA fragments (e.g., PCR-generated sequences and linearized vectors) efficiently and precisely by recognizing a 15-bp overlap at their ends. This 15-bp overlap can be engineered by designing primers for amplification of the desired sequences. The In-Fusion Snap Assembly Master Mix offers high cloning efficiency, especially for longer fragments, short oligonucleotides, and multiple fragments. It is provided in a convenient, lyophilized (dry) format.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Back

The In-Fusion cloning protocol

The In-Fusion cloning protocol

The In-Fusion cloning protocol.

Back

638955: In-Fusion Snap Assembly EcoDry Master Mix

638955: In-Fusion Snap Assembly EcoDry Master Mix
638956 In-Fusion® Snap Assembly EcoDry™ Master Mix 96 Rxns USD $1270.00

The In-Fusion Snap Assembly EcoDry Master Mix enables high-efficiency, high-fidelity, directional cloning of one or more PCR fragments into any vector. The cornerstone of In-Fusion Cloning technology is Takara Bio’s proprietary In-Fusion master mix, which fuses DNA fragments (e.g., PCR-generated sequences and linearized vectors) efficiently and precisely by recognizing a 15-bp overlap at their ends. This 15-bp overlap can be engineered by designing primers for amplification of the desired sequences. The In-Fusion Snap Assembly Master Mix offers high cloning efficiency, especially for longer fragments, short oligonucleotides, and multiple fragments. It is provided in a convenient, lyophilized (dry) format.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Back

The In-Fusion cloning protocol

The In-Fusion cloning protocol

The In-Fusion cloning protocol.

Back

638956: In-Fusion Snap Assembly EcoDry Master Mix

638956: In-Fusion Snap Assembly EcoDry Master Mix

*You must be logged in to a Purchasing Account in order to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@takarabio.com.


In-Fusion Cloning resources

  • In-Fusion Primer Design Tool
  • In-Fusion molar ratio calculator
  • In-Fusion Cloning primer design tutorial
  • Simulate your construct
  • Seamless cloning primer design
  • In‑Fusion Cloning FAQs
  • Choosing a seamless cloning method
  • In-Fusion Cloning tech notes
  • Efficient multiple-fragment cloning
  • Mutagenesis with In-Fusion Cloning

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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2025 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

Takara Bio

Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

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  • Download qPCR resources
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  • Guest webinar: extraction-free SARS-CoV-2 detection
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  • Identifying and cloning protein targets
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  • Pathogen detection
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  • Detection methods
  • Identification and characterization
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  • Antibiotic-resistant bacteria
  • Food crop pathogens
  • Waterborne disease outbreaks
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  • T-cell therapy
  • Antibody therapeutics
  • T-cell receptor profiling
  • TBI initiatives in cancer therapy
  • Cancer research
  • Kickstart your cancer research with long-read sequencing
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer transcriptome analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
  • Alzheimer's disease research
  • Antibody engineering
  • Sample prep from FFPE tissue
  • Single-cell sequencing
  • Reproductive health technologies
  • Embgenix FAQs
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