NucleoSpin Gel and PCR Clean-up (50) 50 preps for PCR clean-up and gel extraction - NucleoSpin Gel and PCR Clean-up Columns, Collection Tubes, buffers

Stellar Competent Cells are an E. coli HST08 strain that provides high transformation efficiency. These cells can be used in a wide variety of applications—from preparation of cDNA and genomic libraries, to construction of longer-length genomic libraries, to subcloning, and even methylated DNA cloning. Stellar Competent Cells lack the gene cluster for cutting foreign methylated DNA (mrr-hsdRMS-mcrBC and mcrA), and are therefore useful for cloning methylated DNA. The cells can also be used for blue/white screening (i.e., α-complementation) when transformed with vectors containing the lacZα gene. A pUC19 vector is provided as well as SOC Medium. Stellar Competent Cells are recommended for use with Clontech's In-Fusion PCR Cloning Kits.

PrimeSTAR Max DNA Polymerase is a B-family polymerase blend that delivers exceptional fidelity and ultra-fast extension speed, making it ideal for applications such as cloning, mutagenesis, and high-throughput PCR. Includes a 2X premix that contains all necessary components (enzyme, buffer, dNTP mix) for assay setup. Primers and template not included.