Purification of RNA from cells and tissues—NucleoSpin RNA Plus
The NucleoSpin RNA Plus kit is a next-generation RNA purification kit designed for quick, efficient RNA isolation from cells and tissues. This kit integrates a new feature, the NucleoSpin gDNA Removal Column, a mini spin column which quickly and efficiently removes genomic DNA contamination, thus eliminating the need for time-consuming rDNase digestion.
The NucleoSpin RNA Plus kit is a next-generation RNA purification kit designed for quick, efficient RNA isolation from cells and tissues. This kit integrates a new feature, the NucleoSpin gDNA Removal Column, a mini spin column which quickly and efficiently removes genomic DNA contamination, thus eliminating the need for time-consuming rDNase digestion.
Cells and tissues are lysed in a lysis buffer that does not require the addition of beta-mercaptoethanol or TCEP since it contains a chaotropic salt that immediately inactivates any RNases. The lysate is added to a NucleoSpin gDNA Removal Column for clarification and removal of contaminating gDNA. After addition of the binding solution to the flowthrough, the RNA is bound to a NucleoSpin RNA Plus Column. Two washes are performed to remove salts, metabolites, and macromolecular cellular components, and high-quality RNA is eluted with RNase-free water.
If you are unsure which RNA purification kit to choose, try our RNA purification kit finder tool and filter products by sample type, kit format, and application.
Overview
- Next-generation kit—provides quick, efficient RNA isolation from cells and tissues
- NucleoSpin gDNA Column—eliminates the need for time-consuming rDNase digestion
- Convenient handling—one-step lysate clearing and gDNA removal using a single column
- Efficient lysis buffer—no beta-mercaptoethanol or TCEP needed
More Information
| Technology | Two-column silica-membrane system 1. Column for DNA removal 2. Column for RNA isolation |
| Format | Mini spin columns |
| Starting material | <1 x 107 cultured cells, <109 bacterial cells, <108 yeast cells, <30 mg human/animal tissue |
| Fragment size | >200 nt |
| Typical yield | 5 x 106 HeLa cells: 40–60 µg 20 mg mouse liver: 80–100 µg 20 mg mouse kidney: 40–70 µg 5 mg mouse spleen: 30–60 µg |
| A260/A280 | 1.9–2.1 |
| A260/A230 | 1.8–2.5 |
| Typical RIN (RNA Integrity Number) | >9 |
| Elution volume | 30–120 µl |
| Preparation time | 20 min/6 preps |
| Binding capacity | 200 µg |
Applications
- RNA isolation from cultured cells, animal tissues, plant tissues, microorganisms
- Real-time RT-PCR, Northern blotting, primer extension, array technology, and RNase protection assays
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
The better way to isolate RNA
Critical factors when purifying RNA are the complete removal of contaminating DNA (gDNA) and the immediate prevention of degradation of RNA. The NucleoSpin RNA Plus kit introduces the NucleoSpin gDNA Removal Column, a spin column which quickly and completely removes genomic DNA contamination without the need for DNase digestion. To maintain RNA integrity, cells and tissues are first lysed by incubation in a chaotropic ion lysis buffer solution, which immediately inactivates RNases.
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