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Endotoxin-free plasmid DNA purification—NucleoBond EF and RS buffers

Buffers needed for endotoxin-free plasmid isolation using NucleoBond Xtra Midi/Maxi*, NucleoBond AX†, or NucleoBond RS columns. Please click "More" for listings of the required and recommended buffers for each column type.

*These columns are used in the NucleoBond Xtra EF kits.
†These columns are used in the NucleoBond PC kits.

Buffers needed for endotoxin-free plasmid isolation or post-IVT RNA cleanup using NucleoBond Xtra Midi/Maxi*, NucleoBond AX†, or NucleoBond RS columns. Please click "More" for listings of the required and recommended buffers for each column type.

*These columns are used in the NucleoBond Xtra EF kits.
†These columns are used in the NucleoBond PC kits.

NucleoBond Xtra Midi/Maxi column buffers

Product name Size Specification Cat. #
Buffer H2O-EF 1,000 mL Water for reconstituting DNA 740798.1
Buffer TE-EF 1,000 mL Slightly alkaline, low-salt buffer for reconstituting DNA 740797.1
Buffer ELU-EF 900 mL Elution buffer 740393.900
Buffer WASH-EF 1,000 mL Wash buffer 740392.1000
Buffer ENDO-EF 1,000 mL Wash buffer ENDO 740391.1000
Buffer FIL-EF 600 mL Filter wash buffer 740390.600
Buffer EQU-EF 1,000 mL Equilibration buffer 740389.1000
Buffer NEU-EF 1,000 mL Neutralization buffer 740388.1000
Buffer LYS-EF 1,000 mL Lysis buffer 740387.1000
Buffer RES-EF 1,000 mL Resuspension buffer, without RNase A 740386.1000

NucleoBond AX column buffers

Product name Size Specification Cat. #
Buffer S1-EF 1,000 mL Resuspension buffer, without RNase A 740790.1
Buffer S2-EF 1,000 mL Lysis buffer 740791.1
Buffer S3-EF 1,000 mL Neutralization buffer 740792.1
Buffer N2-EF 1,000 mL Equilibration buffer 740793.1
Buffer N3-EF 1,000 mL Wash buffer 740794.1
Buffer N4-EF 1,000 mL Wash buffer 740795.1
Buffer N5-EF 600 mL Elution buffer 740796.1

NucleoBond RS column buffers

Product name Size Specification Cat. #
Buffer LYS-EF 1,000 mL Lysis buffer 740387.1000
Buffer NEU-EF 1,000 mL Neutralization buffer 740388.1000
Buffer EQ-EF 1,000 mL
3,000 mL		 Equilibration buffer 740380.1000
740380.3000
Buffer ENTO-EF 1,000 mL
3,000 mL		 Endotoxin removal buffer 740381.1000
740381.3000
Buffer WASH-EF 1,000 mL Wash buffer 740392.1000
Buffer ELU-EF 900 mL Elution buffer 740393.900
Buffer BMC-EF 1,000 mL
3,000 mL		 Binding mediating reconstitution buffer for RNAse-free plasmid purification  740382.1000
740382.3000
Buffer NRB 1,000 mL
3,000 mL		 Binding buffer for RNA cleanup 740371.1000
740371.3000
Buffer EQU* 1,000 mL Equilibration buffer for RNA cleanup 740317.1000
Buffer NRW 1,000 mL
3,000 mL		 Wash buffer for RNA cleanup 740372.1000
740372.3000
Buffer NRE 1,000 mL
3,000 mL		 Elution buffer for RNA cleanup 740373.1000
740373.3000
Buffer RES-EF 1,000 mL Resuspension buffer, without RNase A 740386.1000
Buffer TE-EF 1,000 mL Slightly alkaline, low-salt buffer for reconstituting DNA 740797.1
Buffer H2O-EF 1,000 mL Water for reconstituting DNA 740798.1

*To purchase Buffer EQU, please refer to the NucleoBond buffers product page.

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Cat. # Product Size Price License Quantity Details
740798.1 Buffer H2O-EF 1,000 mL USD $64.00

Endotoxin-free water for reconstituting plasmid DNA. 

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740797.1 Buffer TE-EF 1,000 mL USD $84.00

Endotoxin-free TE buffer reconstituing plasmid DNA.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740393.900 Buffer ELU-EF 900 mL USD $106.00

Elution buffer for use with NucleoBond Xtra Midi/Maxi and NucleoBond RS columns.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740392.1000 Buffer WASH-EF 1,000 mL USD $106.00

Wash buffer for use with NucleoBond Xtra Midi/Maxi and NucleoBond RS columns.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740391.1000 Buffer ENDO-EF 1,000 mL USD $98.00

Wash buffer ENDO for use with NucleoBond Xtra Midi/Maxi columns.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740390.600 Buffer FIL-EF 600 mL USD $68.00

Filter wash buffer for use with NucleoBond Xtra Midi/Maxi columns.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740389.1000 Buffer EQU-EF 1,000 mL USD $106.00

Equilibration buffer for use with NucleoBond Xtra Midi/Maxi columns.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740388.1000 Buffer NEU-EF 1,000 mL USD $98.00

Neutralization buffer for use with NucleoBond Xtra Midi/Maxi or NucleoBond RS columns.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740387.1000 Buffer LYS-EF 1,000 mL USD $98.00

Lysis buffer for use with NucleoBond Xtra Midi/Maxi and NucleoBond RS columns.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740386.1000 Buffer RES-EF 1,000 mL USD $98.00

Resuspension buffer for use with NucleoBond Xtra Midi/Maxi and NucleoBond RS columns.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740790.1 Buffer S1-EF 1,000 mL USD $68.00

Resuspension buffer (without RNase A), for endotoxin-free plasmid purification using NucleoBond AX maxi/mega/giga columns. Cat. # 740790.1 consists of a bottle containing 1,000 ml of buffer.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740791.1 Buffer S2-EF 1,000 mL USD $68.00

Lysis buffer for endotoxin-free plasmid purification using NucleoBond AX maxi/mega/giga columns. Cat. # 740791.1 consists of a bottle containing 1,000 ml of buffer.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data
740792.1 Buffer S3-EF 1,000 mL USD $126.00

Neutralization buffer for endotoxin-free plasmid purification using NucleoBond AX maxi/mega/giga columns. Cat. # 740792.1 consists of a bottle containing 1,000 ml of buffer.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data
740793.1 Buffer N2-EF 1,000 mL USD $127.00

Equilibration buffer for endotoxin-free plasmid purification using NucleoBond AX maxi/mega/giga columns. Cat. # 740793.1 consists of a bottle containing 1,000 ml of buffer.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data
740794.1 Buffer N3-EF 1,000 mL USD $92.00

Wash buffer for endotoxin-free plasmid purification using NucleoBond AX maxi/mega/giga columns. Cat. # 740794.1 consists of a bottle containing 1,000 ml of buffer.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740795.1 Buffer N4-EF 1,000 mL USD $92.00

Wash buffer for endotoxin-free plasmid purification using NucleoBond AX maxi/mega/giga columns. Cat. # 740795.1 consists of a bottle containing 1,000 ml of buffer.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740796.1 Buffer N5-EF 600 mL USD $56.00

Elution buffer for endotoxin-free plasmid purification using NucleoBond AX maxi/mega/giga columns. Cat. # 740796.1 consists of a bottle containing 600 ml of buffer.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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740380.1000 Buffer EQ-EF 1000 ml USD $106.00

Equilibration buffer for use with NucleoBond RS columns.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents
740380.3000 Buffer EQ-EF 3000 ml USD $321.00

Equilibration buffer for use with NucleoBond RS columns.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents
740381.1000 Buffer ENTO-EF 1000 ml USD $106.00

Endotoxin removal buffer for use with NucleoBond RS columns.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents
740381.3000 Buffer ENTO-EF 3000 ml USD $321.00

Endotoxin removal buffer for use with NucleoBond RS columns.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents
740371.1000 Buffer NRB 1,000 mL USD $106.00

Binding buffer for use with NucleoBond RS columns for RNA clean-up.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents
740371.3000 Buffer NRB 3,000 mL USD $321.00

Binding buffer for use with NucleoBond RS columns for RNA clean-up.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents
740372.3000 Buffer NRW 3,000 mL USD $321.00

Wash buffer for use with NucleoBond RS columns for RNA clean-up.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents
740372.1000 Buffer NRW 1,000 mL USD $106.00

Wash buffer for use with NucleoBond RS columns for RNA clean-up.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents
740373.1000 Buffer NRE 1,000 mL USD $106.00

Elution buffer for use with NucleoBond RS columns for RNA clean-up.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents
740373.3000 Buffer NRE 3,000 mL USD $321.00

Elution buffer for use with NucleoBond RS columns for RNA clean-up.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents
740382.1000 Buffer BMC-EF 1000 mL USD $106.00

Binding mediating reconstitution for use with NucleoBond RS columns for the RNase-free plasmid purification.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
740382.3000 Buffer BMC-EF 3000 mL USD $321.00

Binding mediating reconstitution for use with NucleoBond RS columns for the RNase-free plasmid purification.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

More Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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