Enrichment of ThruPLEX libraries with Agilent SureSelect platforms is easily performed. The chart below details the reagents necessary for this SureSelectXT2 protocol. The modules marked in red are not required when integrating with ThruPLEX kits. This target enrichment protocol is compatible with ThruPLEX DNA-Seq FLEX, ThruPLEX Tag-Seq FLEX, and the ThruPLEX DNA-Seq Kit.
Integration of SureSelectXT2 with ThruPLEX kits | |||
---|---|---|---|
Additional reagents | Primers | Required | Illumina P5 and P7 primers |
Blocking oligos | Required | xGen Universal Blocking Oligos (TS HT-i5 and TS HT-i7) | |
Agilent Herculase II Fusion DNA Polymerase | Required | Agilent Cat. # 600677, 600679 (with dNTPs) | |
Agilent SureSelectXT2 Reagent Kit | SureSelectXT2 Library Prep Kit, ILM | Not used |
Replace with ThruPLEX DNA-Seq FLEX, ThruPLEX Tag-Seq or the ThruPLEX DNA-Seq Kit. Contact Agilent to purchase a Reagent Kit without this component. |
SureSelectXT2 Pre-capture Indexes, ILM | Not used | Indexes are included in the ThruPLEX kit | |
SureSelectXT2 Pre-capture Box #1 | Required | ||
SureSelectXT2 Pre-capture Box #2 | Required |