Buffers for isolation of genomic DNA

Choose lysis, wash, and binding buffers for genomic DNA isolation using NucleoSpin kits.

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In accordance with Macherey-Nagel's Quality Management System, each lot of product is tested against predetermined specifications to ensure consistent product quality.

Buffer sets for genomic DNA isolation

Product name	Size	Specification	Cat. #
NucleoBond Buffer Set III	1 set	For isolation of genomic DNA from bacteria & yeast. Only applicable to NucleoBond AXG columns. Set includes RNase A.	740603
NucleoBond Buffer Set IV	1 set	For isolation of genomic DNA from tissue. Only applicable to NucleoBond AXG columns. Set includes Proteinase K & RNase A.	740604
NucleoSpin DNA Trace Bone Buffer Set	25 Preps	For isolation of DNA from bones. Only applicable to NucleoSpin DNA Trace kits, sufficient for 25 preparations.	740943.25
Buffer Set C2/C3	1 set	Binding buffer (for 125 ml of Buffer C4).	740935
Buffer Set PL2/PL3	1 set	Lysis buffer. 100 ml Buffer PL2 and 25 ml Buffer PL3.	740919

Buffers for processing FFPE samples

Product name	Size	Specification	Cat. #
Decrosslink Buffer D-Link	30 ml	Decrosslinking buffer	740979.30
Paraffin Dissolver	25 ml	Deparaffinization buffer	740968.25
Paraffin Dissolver (blue)	60 ml	Deparaffinization buffer	740343.60

Elution buffers and additives for genomic DNA isolation

Product name	Size	Specification	Cat. #
Buffer BE	100 ml	Elution buffer	740306.100
Buffer MC6	1,000 ml	Elution buffer	744861.1
Enhancer SX	50 ml	Additive	740784.50

Binding buffers for genomic DNA isolation

Product name	Size	Specification	Cat. #
Buffer C4	250 ml	Binding buffer	740366.250
Buffer MC2	1,000 ml	Binding buffer	744857.1
Buffer PC	125 ml	Binding buffer	740937
Buffer SB	50 ml	Binding buffer	740785.50
Buffer MB2	100 ml	Binding buffer	744851.80
Buffer BB	22 ml	Binding buffer to be used with NucleoSpin cfDNA XS kits	740394.22
Buffer BB	110 ml	Binding buffer to be used with NucleoSpin cfDNA XS kits	740394.110

Lysis buffers for genomic DNA isolation

Product name	Size	Specification	Cat. #
Buffer B3	100 ml	Lysis buffer	740920
Buffer BQ1	125 ml	Lysis buffer	740923
Buffer C1	100 ml	Lysis buffer	740930
Buffer CF	1,000 ml	Lysis buffer	740946
Buffer MC1	1,000 ml	Lysis buffer	744856.1
Buffer PL1	125 ml	Lysis buffer	740918
Buffer PL2	1,000 ml	Lysis buffer	740351.1000
Buffer SL1	30 ml	Lysis buffer	740781.30
Buffer SL2	30 ml	Lysis buffer	740782.30
Buffer SL3	50 ml	Lysis buffer	740783.50
Buffer T1	25 ml	Lysis buffer	740940.25
Buffer T1	1,000 ml	Lysis buffer	740940.1000
Buffer VL	200 ml	Lysis buffer	740833.200

Precipitation buffers for genomic DNA isolation

Product name	Size	Specification	Cat. #
Buffer PL3	1,000 ml	Precipitation buffer for isolation of genomic DNA from plant samples	740352.1000
Buffer PREC	50 ml	Precipitation of eDNA from water samples; for use with the NucleoSpin eDNA Water kit	740568

Wash buffers for genomic DNA isolation

Product name	Size	Specification	Cat. #
Buffer B5 Concentrate	20 ml	Wash buffer concentrate (for 100 ml of Buffer B5)	740921
Buffer B5 Concentrate	100 ml	Wash buffer concentrate (for 500 ml of Buffer B5)	740921.100
Buffer BW	100 ml	Wash buffer	740922
Buffer BW	500 ml	Wash buffer	740922.500
Buffer CW	100 ml	Wash buffer	740932
Buffer MC3	1,000 ml	Wash buffer	744858.1
Buffer MC4	1,000 ml	Wash buffer	744859.1
Buffer MC5	1,000 ml	Wash buffer	744860.1
Buffer PW1	125 ml	Wash buffer	740938
Buffer PW2 Concentrate	50 ml	Wash buffer concentrate (for 250 ml of Buffer PW2)	740939

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the product User Manual for protocols. Certificates of Analysis and User Manuals are located under the Documents tab.

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