Download supplemental files for SMART-Seq mRNA Long Read data analysis

Thank you for your interest in SMART-Seq mRNA Long Read!

Download the supporting files to process your data

Click on the icon to download the additional files required to demultiplex your SMART-Seq mRNA LR data.

Linux configuration files for Dorado

Please refer to the directions in the Protocol-At-A-Glance for how to use these files in your analysis efforts.

Supported operating systems & hardware guidelines

  • These instructions have been tested on Linux RedHat 9.5
  • For more information about hardware requirements, refer to the official Dorado documentation

Third-party software dependencies*

  • dorado 0.8.3+98456f7, 0.9.0+9dc15a8, 0.9.1+c8c2c9f, or higher (Github link)
  • Optional, but recommended: Restrander (Github link)

*Not included with the downloads; must be downloaded and installed separately.


Generate high-quality, long-read, barcoded cDNA from 1–1,000 intact cells or 10 pg–100 ng of total RNA.

Facilitating the discovery of complex isoforms and novel structural variants from 10 pg–100 ng of total RNA