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In-Fusion molar ratio calculator
The tool below will help you to calculate optimal amounts of vector and insert for the In-Fusion Cloning reaction. All you need to do is enter your vector and insert sizes in base pairs.
- We recommend using at least 50 ng of a DNA insert in your cloning reactions.
- The molar ratio calculator is optimized for use with standard-sized vectors and inserts. Typically, In-Fusion Cloning reactions work well with 100 ng of a 4- to 5-kb linearized vector plus 50 ng of a 1-kb PCR product. If your vector and PCR product differ greatly in size (e.g., you are using a 12-kb vector and 60-bp insert), the molar ratio calculator may recommend using less than 50 ng of insert. If this is the case, you will need to adjust the amount of DNA to ensure that a minimum of 50 ng of insert is used. Please refer to In-Fusion Cloning User Manual for more detailed recommendations.
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