EASY Dilution (for Real Time PCR) solution
The EASY Dilution (for Real Time PCR) solution may be used for diluting DNA and RNA for qPCR and for preparing serial dilutions of the nucleic acid standard when generating a standard curve in qPCR. The product also serves as an excellent dilution buffer for intercalating green dye or probe detection. When diluting DNA and RNA for qPCR in sterilized water or TE buffer, some template absorption to the inside of the tube may occur, preventing correct dilution. The EASY Dilution (for Real Time PCR) solution helps eliminate this absorption problem, allowing for correct dilution, especially at lower DNA and RNA concentrations. Additionally, this product is RNA-free, eliminating the issue of nonspecific RNA-derived amplification.
- Diluting DNA and RNA for qPCR
- Preparing standard for standard curve
Fukuda, S., Takao, S., Kuwayama, M., Shimazu, Y. & Miyazaki, K. Rapid detection of norovirus from fecal specimens by real-time reverse transcription-loop-mediated isothermal amplification assay. J. Clin. Microbiol. 44, 1376–81 (2006).
Miyahara, M. et al. Potential of aerobic denitrification by Pseudomonas stutzeri TR2 to reduce nitrous oxide emissions from wastewater treatment plants. Appl. Environ. Microbiol. 76, 4619–25 (2010).
Morishima, N., Nakanishi, K. & Nakano, A. Activating transcription factor-6 (ATF6) mediates apoptosis with reduction of myeloid cell leukemia sequence 1 (Mcl-1) protein via induction of WW domain binding protein 1. J. Biol. Chem. 286, 35227–35 (2011).
Taguchi, Y. et al. Impaired insulin secretion from the pancreatic islets of hypothyroidal growth-retarded mice. J. Endocrinol. 206, 195–204 (2010).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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