Stem cell pluripotency status check kit with PCR pimers

The Pluripotency Check PCR Kit contains all the necessary reagents to confirm that your mouse embryonic stem (ES) or induced pluripotent (iPS) cells are indeed pluripotent. It includes 16 prevalidated forward and reverse primer mixes, based on nine genes expressed in pluripotent stem cells and two control genes. The different amplicon sizes allow you to distinguish between endogenous and exogenous expression.

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Cat. #	Product	Size	Price
631966	Pluripotency Check PCR Primer Set	16 x 25 Rxns	USD $494.00
Validating the pluripotent status (ability to differentiate into specialized cells of all three germ layers) of your culture is a critical step in stem cell research. The Pluripotency Check PCR Primer Set consists of 16 forward and reverse primer mixes, based on nine genes expressed in pluripotent stem cells, plus two control genes. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Confirming the pluripotent status of an mES culture Confirming the pluripotent status of an mES culture. Total RNA was extracted from ES-E14TG2a mouse embryonic stem (mES) cells, followed by cDNA synthesis and amplification according to the protocol outlined in PT5086-2. M: 100 bp DNA Marker. Lane1, Total Oct3/4. Lane 2: Endogenous Oct3/4. Lane 3: Total Nanog. Lane 4: Endogenous Nanog. Lane 5: Total c-Myc. Lane 6: Endogenous c-Myc. Lane 7: Total Sox2. Lane 8: Endogenous Sox2. Lane 9: Total Klf4. Lane 10: Ecat1. Lane 11: ERas. Lane 12: Esg1. Lane 13: Rex1. Lane 14: GAPDH (control). Lane 15: Beta-Actin (control). Endogenous Klf4, data not shown.
631965	Pluripotency Check PCR Kit	Each	USD $788.00
The Pluripotency Check PCR Kit can be used to validate the pluripotent status (ability to differentiate into specialized cells of all three germ layers) of your stem cell culture. It includes 16 forward and reverse primer mixes, based on nine genes expressed in pluripotent stem cells and two control genes, plus the reagents necessary for RNA extraction, first-strand cDNA synthesis, and PCR. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Confirming the pluripotent status of an mES culture Confirming the pluripotent status of an mES culture. Total RNA was extracted from ES-E14TG2a mouse embryonic stem (mES) cells, followed by cDNA synthesis and amplification according to the protocol outlined in PT5086-2. M: 100 bp DNA Marker. Lane1, Total Oct3/4. Lane 2: Endogenous Oct3/4. Lane 3: Total Nanog. Lane 4: Endogenous Nanog. Lane 5: Total c-Myc. Lane 6: Endogenous c-Myc. Lane 7: Total Sox2. Lane 8: Endogenous Sox2. Lane 9: Total Klf4. Lane 10: Ecat1. Lane 11: ERas. Lane 12: Esg1. Lane 13: Rex1. Lane 14: GAPDH (control). Lane 15: Beta-Actin (control). Endogenous Klf4, data not shown.

Overview

Validate the pluripotent status of your ES or iPS cell culture using a simple PCR kit

16 prevalidated PCR primer mixes
Complete kit with RNA extraction, reverse transcription, and PCR reagents
Confirm your iPS cells by differentiating between endogenous and exogenous gene expression

More Information

Applications

Validating mouse embryonic stem cell status
Confirming iPS status

Pluripotency check PCR primers

Primer set	Amplicon size	Primer set	Amplicon size
Oct3/4 (Total)	485 bp	Klf4	739 bp
Oct3/4 (Endogenous)	224 bp	Klf4 (Endogenous)	206 bp
Nanog (Total)	364 bp	Ecat1	164 bp
Nanog (Endogenous)	223 bp	ERas1	210 bp
Sox2 (Total)	193 bp	Esg1	376 bp
Sox2 (Endogenous)	297 bp	Rex1	287 bp
c-Myc (Total)	228 bp	Beta-Actin (Control)	603 bp
c-Myc (Endogenous)	170 bp	GAPDH (Control)	568 bp

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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