Detect feeder cells contaminating stem cell cultures
The Mouse Feeder Cell Quantification Kit is designed to assess stem cell culture quality by measuring mouse feeder cell (MEF) contamination in stem cell cultures. Mouse feeder cells are used to support the growth and cultivation of stem cells such as hESCs (human embryonic stem cells) and hiPSCs (human induced pluripotent stem cells), but they must be removed before use in certain applications, such as regenerative medicine.
The Mouse Feeder Cell Quantification Kit is designed to assess stem cell culture quality by measuring mouse feeder cell (MEF) contamination in stem cell cultures. Mouse feeder cells are used to support the growth and cultivation of stem cells such as hESCs (human embryonic stem cells) and hiPSCs (human induced pluripotent stem cells), but they must be removed before use in certain applications, such as regenerative medicine. This kit provides highly sensitive detection and quantification of genomic DNA derived from residual mouse feeder cells by using real-time PCR to analyze genomic DNA extracted from stem cell cultures.
The Mouse Feeder Cell Quantification Kit includes real-time PCR primers that detect a mouse mitochondrial (mt) DNA sequence, providing highly sensitive detection of mouse feeder cells. The kit also includes nuclear DNA PCR primers for normalization in order to quantify the mouse mtDNA copy number, which can vary between different mouse feeder cell lots, depending on the cell type, cell line, and differentiation state. Calculating the mouse mtDNA copy number makes it possible to compare the levels of mouse cell contamination in stem cell cultures grown with different lots of mouse feeder cells.
Overview
- Utilizes real-time PCR to detect and quantify residual mouse feeder cells
- Provides mtDNA PCR primers to make mouse mtDNA sequence detection easy and sensitive
- Includes nuclear DNA PCR primers for normalization to quantify mouse mtDNA copy number
More Information
Applications
- Assess the level of mouse cell contamination in stem cell cultures grown using mouse feeder cells
- Compare the levels of mouse cell contamination in stem cell cultures grown with different lots of mouse feeder cells
Components
| TB Green Premix Ex Taq II (Tli RNaseH Plus) (2X Conc.) | 625 μl x 4 |
| Mouse_Mt_primer (10 μM each) | 100 μl |
| Mouse_genome_primer (10 μM each) | 100 μl |
| Human_primer_Type I (CHR3) (10 μM each) | 100 μl |
| Human_primer_Type II (CHR7) (10 μM each) | 100 μl |
| Human_primer_Type III (CHR15) (10 μM each) | 100 μl |
| dH2O | 1 ml x 2 |
| EASY Dilution (for Real Time PCR) | 1 ml x 2 |
| ROX Reference Dye (50X Conc.) | 50 μl |
| ROX Reference Dye II (50X Conc.) | 50 μl |
| Human Genomic DNA (100 ng/μl) | 20 μl |
Storage
- –20°C (all components except Premix Ex Taq II (Tli RNaseH Plus))
- –80°C (Premix Ex Taq II (Tli RNaseH Plus))
- Product is stable for up to 6 months when stored at 4°C. Protect from light and avoid contamination. This kit is shipped in a dry ice-packed container. For long-term storage, keep item at –80°C.
- Do not store at –20°C. Store thawed product at 4°C and use within 6 months.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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