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  • ‹ Back to Tet-On 3G systems
  • Tet-On 3G systems
  • Tet-On 3G—lentiviral
  • Tet-On 3G—retroviral
  • Adeno-X Tet-On 3G inducible expression system
  • Tet-On 3G cell lines
  • Tet-On 3G systems—bidirectional
  • Tet-On 3G systems—bicistronic
  • Tet-On 3G systems—EF1-alpha promoter
Selection guides Tet selection guide
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Home › Products › Gene function › Tet-inducible expression systems › Tet-On 3G systems › Tet-On 3G systems

Tet-inducible expression systems

  • Tet-On 3G systems
    • Tet-On 3G systems
    • Tet-On 3G—lentiviral
    • Tet-On 3G—retroviral
    • Adeno-X Tet-On 3G inducible expression system
    • Tet-On 3G cell lines
    • Tet-On 3G systems—bidirectional
    • Tet-On 3G systems—bicistronic
    • Tet-On 3G systems—EF1-alpha promoter
  • Tet systems legacy products
    • Tet-On and Tet-Off—1st generation
    • Tet-Off cell lines
    • Tet-On and Tet-Off—2nd generation
    • Tet-tTS transcriptional silencer
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Selection guides Tet selection guide
Overviews Tet-One overview

Tet-On 3G tetracycline-inducible expression systems

Tet-On 3G inducible gene expression systems

Tet-On 3G tetracycline-inducible gene expression systems are the third generation of the most powerful, versatile, and widely cited inducible mammalian expression systems available. The Tet-On 3G system offers a significant improvement over Tet-On and Tet-On Advanced systems with significantly reduced basal expression and increased sensitivity to doxycycline, a tetracycline analogue.

Tet-On 3G tetracycline-inducible gene expression systems are the third generation of the most powerful, versatile, and widely cited inducible mammalian expression systems available. The Tet-On 3G system offers a significant improvement over Tet-On and Tet-On Advanced systems with significantly reduced basal expression and increased sensitivity to doxycycline, a tetracycline analogue.

Learn more about the high sensitivity of the transactivator, and the low background from the tight promoter in the Tet system learning center.

Choice of Tet-On 3G vector formats

There is a range of available Tet-On 3G vector formats:

  • Tet-One systems (listed elsewhere) provide a complete inducible expression system on a single vector.
  • The Tet-On 3G Inducible Expression System (EF1alpha Version), our EF1-alpha promoter version of the Tet-On 3G system, provides consistent long-term expression of the Tet-On 3G transactivator, even in cell types known for their tendency to silence a CMV promoter over time, such as hematopoietic cells and stem cells.
  • Bidirectional promoter versions of the system allow you to induce expression of two genes from the same promoter—either two transgenes of your choosing, or a single transgene and a fluorescent protein. If your cells turn red or green after adding Dox, this confirms that your gene has been turned on and that you have selected a high-performing inducible clone.
  • Using IRES technology, we've married very tight gene expression control to a bright red fluorescent protein (mCherry) in the Tet-On 3G Inducible Expression System (with mCherry), and a bright green fluorescent protein (ZsGreen1) in the Tet-On 3G Inducible Expression System (with ZsGreen1). 
  • Retroviral and lentiviral formats include the PTRE3GV promoter, which is optimized for use in retroviral and lentiviral vectors. They are supplied with complete viral packaging systems.

Use tetracycline-approved serum for optimal results

With the greatly increased sensitivity of the Tet-On 3G system, it is more important than ever that the serum you use for your studies is guaranteed to be tetracycline-free. Only Takara Bio performs actual inducibility tests on a sensitive Tet-inducible cell line in order to provide an absolute guarantee that our serum will not affect basal expression in your Tet-On 3G experiments. Each Tet-On 3G system is supplied with 50 ml of our premium Tet System Approved FBS.

 More  Less
Cat. # Product Size Price License Quantity Details
631337 Tet-On® 3G Bidirectional Inducible Expression System Each USD $993.00

License Statement

ID Number  
42 Use of the Tetracycline controllable expression systems (the "Tet Technology") is covered by a series of patents including U.S. Patent # 7541446, # 8383364, # 9181556 , European patents EP # 1200607, # 1954811, #2352833 and corresponding patent claims outside these regions which are proprietary to TET Systems GmbH & Co. KG. Academic research institutions are granted an automatic license with the purchase of this product to use the Tet Technology only for internal, academic research purposes, which license specifically excludes the right to sell, or otherwise transfer, the Tet Technology or its component parts to third parties. Notwithstanding the above, academic and not-for profit research institutions whose research using the Tet Technology is sponsored by for profit organizations, which shall receive ownership to any data and results stemming from the sponsored research, shall need a commercial license agreement from TET Systems in order to use the Tet Technology. In accepting this license, all users acknowledge that the Tet Technology is experimental in nature. TET Systems GmbH & Co. KG makes no warranties, express or implied or of any kind, and hereby disclaims any warranties, representations, or guarantees of any kind as to the Tet Technology, patents, or products. All others are invited to request a license from TET Systems GmbH & Co. KG prior to purchasing these reagents or using them for any purpose. Takara Bio USA, Inc. is required by its licensing agreement to submit a report of all purchasers of the Tet-controllable expression system to TET Systems.

For license information, please contact:
GSF/CEO
TET Systems GmbH & Co. KG,
Im Neuenheimer Feld 582
69120 Heidelberg
Germany
Tel: +49 6221 5880400
Fax: +49 6221 5880404
email: info@tetsystems.com
or use the electronic licensing request form via http://www.tetsystems.com/ip-licensing/licensing/for-profit-research
63 Use of this product is covered by one or more of the following U.S. Patent Nos. and corresponding patent claims outside the U.S.: 6,998,115; 7,427,394. This product is intended for research purposes only. It may not be used for (i) any human or veterinary use, including without limitation therapeutic and prophylactic use, (ii) any clinical use, including without limitation diagnostic use, (iii) screening of chemical and/or biological compounds for the identification of pharmaceutically active agents (including but not limited to screening of small molecules), target validation, preclinical testing services, or drug development. Any use of this product for any of the above mentioned purposes requires a license from the Massachusetts Institute of Technology.
*

The Tet-On 3G Bidirectional Inducible Expression System is a powerful, tightly regulated, tetracycline-inducible mammalian expression system that is controlled by the addition of doxycycline to the culture medium. This system allows the simultaneous expression of two genes of interest from a bidirectional, Tet-responsive promoter that binds the Tet-On 3G transactivator protein in the presence of doxycycline. It includes the pTRE3G-BI Vector Set, consisting of a bidirectional vector into which both genes of interest can be cloned and simultaneously expressed, a bidirectional control vector that contains the luciferase gene in one of the two possible cloning sites, and two linear selection markers for hygromycin and puromycin resistance—and the pCMV-Tet3G Vector, which constitutively expresses the Tet-On 3G transactivator protein from a CMV promoter. This system also includes our highly efficient Xfect Transfection Reagent and Tet System Approved FBS.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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The Tet-On 3G systems allow inducible gene expression only in the presence of doxycycline (Dox)

The Tet-On 3G systems allow inducible gene expression only in the presence of doxycycline (Dox)

The Tet-On 3G systems allow inducible gene expression only in the presence of doxycycline (Dox). When Dox binds, the transactivator undergoes a conformational change allowing it to bind tet operator (tetO) repeats within the TRE3G promoter.

Back

Tet-On 3G inducible expression systems with CMV regulator vectors

Tet-On 3G inducible expression systems with CMV regulator vectors
Tet-On 3G inducible expression systems with CMV regulator vectors.

Back

Doxycycline-regulated expression of two genes

Doxycycline-regulated expression of two genes

Doxycycline-regulated expression of two genes. ZsGreen1 and firefly luciferase were cloned into separate multiple cloning sites that flank the inducible bidirectional promoter in the pTRE3G-BI vector. The plasmid was then cotransfected into HEK 293 cells with pCMV-Tet3G (which expresses the Tet-On 3G transactivator). Cells were treated with the indicated doses of doxycycline, and induced gene expression was measured 24 hr later. Induced expression of ZsGreen1 fluorescent protein was determined by fluorescent microscopy and luciferase activity was measured using a luminometer (RLU = Relative Light Units).

Back

PTRE3G-BI inducible bidirectional promoter shows equivalent induced expression of two genes

PTRE3G-BI inducible bidirectional promoter shows equivalent induced expression of two genes

PTRE3G-BI inducible bidirectional promoter shows equivalent induced expression of two genes. ZsGreen1 and mCherry fluorescent proteins were cloned into separate multiple cloning sites that flank the inducible bidirectional promoter in the pTRE3G-BI vector. The plasmid was then cotransfected into HEK 293 cells with pEF1a-Tet3G (which expresses the Tet-On 3G transactivator). Cells were treated with 100 ng/ml doxycycline and induced expression of the fluorescent proteins was observed 24 hr later. Cells in the mixed population that expressed high levels of ZsGreen1 also showed similarly high levels of mCherry expression, indicating equivalent expression from both sides of the bidirectional promoter.

Required Products

Cat. # Product Size Price License Quantity Details
631106 Tet System Approved FBS 500 mL USD $865.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or its derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems.

Documents Components Image Data

Back

Fetal bovine serum (FBS) approved for use with tetracycline-inducible expression systems

Fetal bovine serum (FBS) approved for use with tetracycline-inducible expression systems

Fetal bovine serum (FBS) approved for use with tetracycline inducible expression systems. Only Takara Bio USA functionally tests FBS to be sure that no contaminating tetracycline derivatives interfere with induced expression levels.

Back

Our fetal bovine serum is functionally tested to guarantee consistent results with tetracycline-inducible expression systems

Our fetal bovine serum is functionally tested to guarantee consistent results with tetracycline-inducible expression systems

Fetal bovine serum from Clontech is functionally tested to guarantee consistent results with tetracycline-inducible expression systems. Serum from other vendors that have not been functionally tested may contain tetracyclines that affect the maximum expression in Tet-Off systems, or generate background in Tet-On systems. Data here shows induction of luciferase expression from our CHO-AA8-Luc Tet-Off Control Cell Line using different sources of FBS.

631101 Tet System Approved FBS, US-Sourced 500 mL USD $875.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. This FBS has been processed in the United States.

Documents Components Image Data

Back

Fetal bovine serum (FBS) approved for use with tetracycline-inducible expression systems

Fetal bovine serum (FBS) approved for use with tetracycline-inducible expression systems

Fetal bovine serum (FBS) approved for use with tetracycline inducible expression systems. Only Takara Bio USA functionally tests FBS to be sure that no contaminating tetracycline derivatives interfere with induced expression levels.

Back

Our fetal bovine serum is functionally tested to guarantee consistent results with tetracycline-inducible expression systems

Our fetal bovine serum is functionally tested to guarantee consistent results with tetracycline-inducible expression systems

Fetal bovine serum from Clontech is functionally tested to guarantee consistent results with tetracycline-inducible expression systems. Serum from other vendors that have not been functionally tested may contain tetracyclines that affect the maximum expression in Tet-Off systems, or generate background in Tet-On systems. Data here shows induction of luciferase expression from our CHO-AA8-Luc Tet-Off Control Cell Line using different sources of FBS.

631338 Tet-On® 3G Bidirectional Inducible Expression System (with mCherry) Each USD $1095.00

License Statement

ID Number  
42 Use of the Tetracycline controllable expression systems (the "Tet Technology") is covered by a series of patents including U.S. Patent # 7541446, # 8383364, # 9181556 , European patents EP # 1200607, # 1954811, #2352833 and corresponding patent claims outside these regions which are proprietary to TET Systems GmbH & Co. KG. Academic research institutions are granted an automatic license with the purchase of this product to use the Tet Technology only for internal, academic research purposes, which license specifically excludes the right to sell, or otherwise transfer, the Tet Technology or its component parts to third parties. Notwithstanding the above, academic and not-for profit research institutions whose research using the Tet Technology is sponsored by for profit organizations, which shall receive ownership to any data and results stemming from the sponsored research, shall need a commercial license agreement from TET Systems in order to use the Tet Technology. In accepting this license, all users acknowledge that the Tet Technology is experimental in nature. TET Systems GmbH & Co. KG makes no warranties, express or implied or of any kind, and hereby disclaims any warranties, representations, or guarantees of any kind as to the Tet Technology, patents, or products. All others are invited to request a license from TET Systems GmbH & Co. KG prior to purchasing these reagents or using them for any purpose. Takara Bio USA, Inc. is required by its licensing agreement to submit a report of all purchasers of the Tet-controllable expression system to TET Systems.

For license information, please contact:
GSF/CEO
TET Systems GmbH & Co. KG,
Im Neuenheimer Feld 582
69120 Heidelberg
Germany
Tel: +49 6221 5880400
Fax: +49 6221 5880404
email: info@tetsystems.com
or use the electronic licensing request form via http://www.tetsystems.com/ip-licensing/licensing/for-profit-research
44 The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,005,511; 7,157,566; 7,393,923 and 7,250,298.
63 Use of this product is covered by one or more of the following U.S. Patent Nos. and corresponding patent claims outside the U.S.: 6,998,115; 7,427,394. This product is intended for research purposes only. It may not be used for (i) any human or veterinary use, including without limitation therapeutic and prophylactic use, (ii) any clinical use, including without limitation diagnostic use, (iii) screening of chemical and/or biological compounds for the identification of pharmaceutically active agents (including but not limited to screening of small molecules), target validation, preclinical testing services, or drug development. Any use of this product for any of the above mentioned purposes requires a license from the Massachusetts Institute of Technology.
72 Living Colors Fluorescent Protein Products:

Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

Not-For-Profit Non-Commercial Use License:
A copy of the Tet-On® 3G Bidirectional Inducible Expression System (with mCherry) product License Agreement can be found by clicking here.
*

The Tet-On 3G Inducible Expression System (with mCherry) is a powerful, tightly regulated, tetracycline-inducible mammalian expression system that is controlled by the addition of doxycycline to the culture medium. This system allows the simultaneous expression of a gene of interest and a red fluorescent protein (mCherry) marker from a bidirectional, Tet-responsive promoter that binds the Tet-On 3G transactivator protein in the presence of doxycycline. It includes the pTRE3G-BI-mCherry Vector Set, consisting of a bidirectional vector into which the gene of interest can be cloned and simultaneously expressed with the mCherry marker, a bidirectional control vector that contains the luciferase gene in one of the two possible cloning sites, and two linear selection markers for hygromycin and puromycin resistance—and the pCMV-Tet3G Vector, which constitutively expresses the Tet-On 3G transactivator protein from a CMV promoter. This system also includes our highly efficient Xfect Transfection Reagent and Tet System Approved FBS.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Back

Tet-On 3G inducible expression systems with CMV regulator vectors

Tet-On 3G inducible expression systems with CMV regulator vectors
Tet-On 3G inducible expression systems with CMV regulator vectors.

Back

Doxycycline-regulated expression of two genes

Doxycycline-regulated expression of two genes

Doxycycline-regulated expression of two genes. ZsGreen1 and firefly luciferase were cloned into separate multiple cloning sites that flank the inducible bidirectional promoter in the pTRE3G-BI vector. The plasmid was then cotransfected into HEK 293 cells with pCMV-Tet3G (which expresses the Tet-On 3G transactivator). Cells were treated with the indicated doses of doxycycline, and induced gene expression was measured 24 hr later. Induced expression of ZsGreen1 fluorescent protein was determined by fluorescent microscopy and luciferase activity was measured using a luminometer (RLU = Relative Light Units).

Back

PTRE3G-BI inducible bidirectional promoter shows equivalent induced expression of two genes

PTRE3G-BI inducible bidirectional promoter shows equivalent induced expression of two genes

PTRE3G-BI inducible bidirectional promoter shows equivalent induced expression of two genes. ZsGreen1 and mCherry fluorescent proteins were cloned into separate multiple cloning sites that flank the inducible bidirectional promoter in the pTRE3G-BI vector. The plasmid was then cotransfected into HEK 293 cells with pEF1a-Tet3G (which expresses the Tet-On 3G transactivator). Cells were treated with 100 ng/ml doxycycline and induced expression of the fluorescent proteins was observed 24 hr later. Cells in the mixed population that expressed high levels of ZsGreen1 also showed similarly high levels of mCherry expression, indicating equivalent expression from both sides of the bidirectional promoter.

Required Products

Cat. # Product Size Price License Quantity Details
631106 Tet System Approved FBS 500 mL USD $865.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or its derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems.

Documents Components Image Data

Back

Fetal bovine serum (FBS) approved for use with tetracycline-inducible expression systems

Fetal bovine serum (FBS) approved for use with tetracycline-inducible expression systems

Fetal bovine serum (FBS) approved for use with tetracycline inducible expression systems. Only Takara Bio USA functionally tests FBS to be sure that no contaminating tetracycline derivatives interfere with induced expression levels.

Back

Our fetal bovine serum is functionally tested to guarantee consistent results with tetracycline-inducible expression systems

Our fetal bovine serum is functionally tested to guarantee consistent results with tetracycline-inducible expression systems

Fetal bovine serum from Clontech is functionally tested to guarantee consistent results with tetracycline-inducible expression systems. Serum from other vendors that have not been functionally tested may contain tetracyclines that affect the maximum expression in Tet-Off systems, or generate background in Tet-On systems. Data here shows induction of luciferase expression from our CHO-AA8-Luc Tet-Off Control Cell Line using different sources of FBS.

631101 Tet System Approved FBS, US-Sourced 500 mL USD $875.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. This FBS has been processed in the United States.

Documents Components Image Data

Back

Fetal bovine serum (FBS) approved for use with tetracycline-inducible expression systems

Fetal bovine serum (FBS) approved for use with tetracycline-inducible expression systems

Fetal bovine serum (FBS) approved for use with tetracycline inducible expression systems. Only Takara Bio USA functionally tests FBS to be sure that no contaminating tetracycline derivatives interfere with induced expression levels.

Back

Our fetal bovine serum is functionally tested to guarantee consistent results with tetracycline-inducible expression systems

Our fetal bovine serum is functionally tested to guarantee consistent results with tetracycline-inducible expression systems

Fetal bovine serum from Clontech is functionally tested to guarantee consistent results with tetracycline-inducible expression systems. Serum from other vendors that have not been functionally tested may contain tetracyclines that affect the maximum expression in Tet-Off systems, or generate background in Tet-On systems. Data here shows induction of luciferase expression from our CHO-AA8-Luc Tet-Off Control Cell Line using different sources of FBS.

631339 Tet-On® 3G Bidirectional Inducible Expression System (with ZsGreen1) Each USD $1095.00

License Statement

ID Number  
42 Use of the Tetracycline controllable expression systems (the "Tet Technology") is covered by a series of patents including U.S. Patent # 7541446, # 8383364, # 9181556 , European patents EP # 1200607, # 1954811, #2352833 and corresponding patent claims outside these regions which are proprietary to TET Systems GmbH & Co. KG. Academic research institutions are granted an automatic license with the purchase of this product to use the Tet Technology only for internal, academic research purposes, which license specifically excludes the right to sell, or otherwise transfer, the Tet Technology or its component parts to third parties. Notwithstanding the above, academic and not-for profit research institutions whose research using the Tet Technology is sponsored by for profit organizations, which shall receive ownership to any data and results stemming from the sponsored research, shall need a commercial license agreement from TET Systems in order to use the Tet Technology. In accepting this license, all users acknowledge that the Tet Technology is experimental in nature. TET Systems GmbH & Co. KG makes no warranties, express or implied or of any kind, and hereby disclaims any warranties, representations, or guarantees of any kind as to the Tet Technology, patents, or products. All others are invited to request a license from TET Systems GmbH & Co. KG prior to purchasing these reagents or using them for any purpose. Takara Bio USA, Inc. is required by its licensing agreement to submit a report of all purchasers of the Tet-controllable expression system to TET Systems.

For license information, please contact:
GSF/CEO
TET Systems GmbH & Co. KG,
Im Neuenheimer Feld 582
69120 Heidelberg
Germany
Tel: +49 6221 5880400
Fax: +49 6221 5880404
email: info@tetsystems.com
or use the electronic licensing request form via http://www.tetsystems.com/ip-licensing/licensing/for-profit-research
63 Use of this product is covered by one or more of the following U.S. Patent Nos. and corresponding patent claims outside the U.S.: 6,998,115; 7,427,394. This product is intended for research purposes only. It may not be used for (i) any human or veterinary use, including without limitation therapeutic and prophylactic use, (ii) any clinical use, including without limitation diagnostic use, (iii) screening of chemical and/or biological compounds for the identification of pharmaceutically active agents (including but not limited to screening of small molecules), target validation, preclinical testing services, or drug development. Any use of this product for any of the above mentioned purposes requires a license from the Massachusetts Institute of Technology.
*

The Tet-On 3G Inducible Expression System (with ZsGreen1) is a powerful, tightly regulated, tetracycline-inducible mammalian expression system that is controlled by the addition of doxycycline to the culture medium. This system allows the simultaneous expression of a gene of interest and a green fluorescent protein (ZsGreen1) marker from a bidirectional, Tet-responsive promoter that binds the Tet-On 3G transactivator protein in the presence of doxycycline. It includes the pTRE3G-BI-ZsGreen1 Vector Set, consisting of a bidirectional vector into which the gene of interest can be cloned and simultaneously expressed with the ZsGreen1 marker, a bidirectional control vector that contains the luciferase gene in one of the two possible cloning sites, and two linear selection markers for hygromycin and puromycin resistance—and the pCMV-Tet3G Vector, which constitutively expresses the Tet-On 3G transactivator protein from a CMV promoter. This system also includes our highly efficient Xfect Transfection Reagent and Tet System Approved FBS.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Back

Tet-On 3G inducible expression systems with CMV regulator vectors

Tet-On 3G inducible expression systems with CMV regulator vectors
Tet-On 3G inducible expression systems with CMV regulator vectors.

Back

Doxycycline-regulated expression of two genes

Doxycycline-regulated expression of two genes

Doxycycline-regulated expression of two genes. ZsGreen1 and firefly luciferase were cloned into separate multiple cloning sites that flank the inducible bidirectional promoter in the pTRE3G-BI vector. The plasmid was then cotransfected into HEK 293 cells with pCMV-Tet3G (which expresses the Tet-On 3G transactivator). Cells were treated with the indicated doses of doxycycline, and induced gene expression was measured 24 hr later. Induced expression of ZsGreen1 fluorescent protein was determined by fluorescent microscopy and luciferase activity was measured using a luminometer (RLU = Relative Light Units).

Back

PTRE3G-BI inducible bidirectional promoter shows equivalent induced expression of two genes

PTRE3G-BI inducible bidirectional promoter shows equivalent induced expression of two genes

PTRE3G-BI inducible bidirectional promoter shows equivalent induced expression of two genes. ZsGreen1 and mCherry fluorescent proteins were cloned into separate multiple cloning sites that flank the inducible bidirectional promoter in the pTRE3G-BI vector. The plasmid was then cotransfected into HEK 293 cells with pEF1a-Tet3G (which expresses the Tet-On 3G transactivator). Cells were treated with 100 ng/ml doxycycline and induced expression of the fluorescent proteins was observed 24 hr later. Cells in the mixed population that expressed high levels of ZsGreen1 also showed similarly high levels of mCherry expression, indicating equivalent expression from both sides of the bidirectional promoter.

Required Products

Cat. # Product Size Price License Quantity Details
631106 Tet System Approved FBS 500 mL USD $865.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or its derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems.

Documents Components Image Data

Back

Fetal bovine serum (FBS) approved for use with tetracycline-inducible expression systems

Fetal bovine serum (FBS) approved for use with tetracycline-inducible expression systems

Fetal bovine serum (FBS) approved for use with tetracycline inducible expression systems. Only Takara Bio USA functionally tests FBS to be sure that no contaminating tetracycline derivatives interfere with induced expression levels.

Back

Our fetal bovine serum is functionally tested to guarantee consistent results with tetracycline-inducible expression systems

Our fetal bovine serum is functionally tested to guarantee consistent results with tetracycline-inducible expression systems

Fetal bovine serum from Clontech is functionally tested to guarantee consistent results with tetracycline-inducible expression systems. Serum from other vendors that have not been functionally tested may contain tetracyclines that affect the maximum expression in Tet-Off systems, or generate background in Tet-On systems. Data here shows induction of luciferase expression from our CHO-AA8-Luc Tet-Off Control Cell Line using different sources of FBS.

631101 Tet System Approved FBS, US-Sourced 500 mL USD $875.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. This FBS has been processed in the United States.

Documents Components Image Data

Back

Fetal bovine serum (FBS) approved for use with tetracycline-inducible expression systems

Fetal bovine serum (FBS) approved for use with tetracycline-inducible expression systems

Fetal bovine serum (FBS) approved for use with tetracycline inducible expression systems. Only Takara Bio USA functionally tests FBS to be sure that no contaminating tetracycline derivatives interfere with induced expression levels.

Back

Our fetal bovine serum is functionally tested to guarantee consistent results with tetracycline-inducible expression systems

Our fetal bovine serum is functionally tested to guarantee consistent results with tetracycline-inducible expression systems

Fetal bovine serum from Clontech is functionally tested to guarantee consistent results with tetracycline-inducible expression systems. Serum from other vendors that have not been functionally tested may contain tetracyclines that affect the maximum expression in Tet-Off systems, or generate background in Tet-On systems. Data here shows induction of luciferase expression from our CHO-AA8-Luc Tet-Off Control Cell Line using different sources of FBS.

631168 Tet-On® 3G Inducible Expression System Each USD $993.00

License Statement

ID Number  
42 Use of the Tetracycline controllable expression systems (the "Tet Technology") is covered by a series of patents including U.S. Patent # 7541446, # 8383364, # 9181556 , European patents EP # 1200607, # 1954811, #2352833 and corresponding patent claims outside these regions which are proprietary to TET Systems GmbH & Co. KG. Academic research institutions are granted an automatic license with the purchase of this product to use the Tet Technology only for internal, academic research purposes, which license specifically excludes the right to sell, or otherwise transfer, the Tet Technology or its component parts to third parties. Notwithstanding the above, academic and not-for profit research institutions whose research using the Tet Technology is sponsored by for profit organizations, which shall receive ownership to any data and results stemming from the sponsored research, shall need a commercial license agreement from TET Systems in order to use the Tet Technology. In accepting this license, all users acknowledge that the Tet Technology is experimental in nature. TET Systems GmbH & Co. KG makes no warranties, express or implied or of any kind, and hereby disclaims any warranties, representations, or guarantees of any kind as to the Tet Technology, patents, or products. All others are invited to request a license from TET Systems GmbH & Co. KG prior to purchasing these reagents or using them for any purpose. Takara Bio USA, Inc. is required by its licensing agreement to submit a report of all purchasers of the Tet-controllable expression system to TET Systems.

For license information, please contact:
GSF/CEO
TET Systems GmbH & Co. KG,
Im Neuenheimer Feld 582
69120 Heidelberg
Germany
Tel: +49 6221 5880400
Fax: +49 6221 5880404
email: info@tetsystems.com
or use the electronic licensing request form via http://www.tetsystems.com/ip-licensing/licensing/for-profit-research
63 Use of this product is covered by one or more of the following U.S. Patent Nos. and corresponding patent claims outside the U.S.: 6,998,115; 7,427,394. This product is intended for research purposes only. It may not be used for (i) any human or veterinary use, including without limitation therapeutic and prophylactic use, (ii) any clinical use, including without limitation diagnostic use, (iii) screening of chemical and/or biological compounds for the identification of pharmaceutically active agents (including but not limited to screening of small molecules), target validation, preclinical testing services, or drug development. Any use of this product for any of the above mentioned purposes requires a license from the Massachusetts Institute of Technology.
*

The Tet-On 3G Inducible Expression System is a powerful, tightly regulated, tetracycline-inducible mammalian expression system that is controlled by the addition of doxycycline to the culture medium. The system includes all necessary vectors, our highly efficient transfection reagent, Xfect, and Tet System Approved FBS.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Back

The Tet-On 3G systems allow inducible gene expression only in the presence of doxycycline (Dox)

The Tet-On 3G systems allow inducible gene expression only in the presence of doxycycline (Dox)

The Tet-On 3G systems allow inducible gene expression only in the presence of doxycycline (Dox). When Dox binds, the transactivator undergoes a conformational change allowing it to bind tet operator (tetO) repeats within the TRE3G promoter.

Back

The Tet-On 3G system is a highly sensitive tetracycline inducible expression system

The Tet-On 3G system is a highly sensitive tetracycline inducible expression system

The Tet-On 3G system is a highly sensitive tetracycline inducible expression system. Following cotransient transfection of pCMV-Tet3G and pTRE3G-Luc in HeLa cells, increasing levels of Dox were added and expression of luciferase was measured using an anti-luciferase antibody (Panel A) and a luciferase assay (Panel B). Induced expression was very high even with Dox concentrations as low as 10 ng/ml, and was detectable even at 0.1 ng/ml Dox.

Back

The Tet-On 3G system results in the lowest background and as a result provides a much higher fold induction than previous generations of the Tet-On tetracycline inducible expression system

The Tet-On 3G system results in the lowest background and as a result provides a much higher fold induction than previous generations of the Tet-On tetracycline inducible expression system

Tet-On 3G results in the lowest background and as a result provides a much higher fold-induction than previous generations of the Tet-On tetracycline-inducible expression system.

Back

The Tet-On 3G system demonstrates higher sensitivity to doxycycline than Tet-On Advanced system

The Tet-On 3G system demonstrates higher sensitivity to doxycycline than Tet-On Advanced system

The Tet-On 3G system demonstrates higher sensitivity to doxycycline than Tet-On Advanced system. Tet-On 3G and Tet-On Advanced genes were integrated at the same locus in a stable HLF33 cell line expressing luciferase from a TRE promoter. For each of these two double-stable cell lines, induced luciferase expression was measured in response to a range of doxycycline (Dox) concentrations. At 5–10 ng/ml Dox, induced expression was 100– to 150-fold higher for the Tet-On 3G cell line, and at 50 ng/ml, expression was 4.6-fold higher (data kindly provided by Professor W. Hillen, and Dr. C. Berens, University of Erlangen). .

Back

The PTRE3G promoter results in significantly reduced basal expression

The PTRE3G promoter results in significantly reduced basal expression

The PTRE3G promoter results in significantly reduced basal expression. HEK 293 cells were transiently cotranstected with both the response vectors (containing luciferase) and regulator vectors from each of the Tet-On 3G and Tet-On advanced inducible expression systems. The cells were cultured in the presence and absence of Dox, and after 24 hr, luciferase expression was measured. Although both systems provided strong expression in the presence of Dox, the Tet-On 3G system produces far lower background expression in the absence of Dox (inset).

Back

Three generations of Tet-On System vectors

Three generations of Tet-On System vectors
Three generations of Tet-On System vectors. The Tet-On 3G inducible expression system offers a significant improvement over Tet-On and Tet-On Advanced by further reducing basal expression and increasing sensitivity to doxycycline.

Back

Tet-On 3G inducible expression systems with CMV regulator vectors

Tet-On 3G inducible expression systems with CMV regulator vectors
Tet-On 3G inducible expression systems with CMV regulator vectors.

Required Products

Cat. # Product Size Price License Quantity Details
631106 Tet System Approved FBS 500 mL USD $865.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or its derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems.

Documents Components Image Data

Back

Fetal bovine serum (FBS) approved for use with tetracycline-inducible expression systems

Fetal bovine serum (FBS) approved for use with tetracycline-inducible expression systems

Fetal bovine serum (FBS) approved for use with tetracycline inducible expression systems. Only Takara Bio USA functionally tests FBS to be sure that no contaminating tetracycline derivatives interfere with induced expression levels.

Back

Our fetal bovine serum is functionally tested to guarantee consistent results with tetracycline-inducible expression systems

Our fetal bovine serum is functionally tested to guarantee consistent results with tetracycline-inducible expression systems

Fetal bovine serum from Clontech is functionally tested to guarantee consistent results with tetracycline-inducible expression systems. Serum from other vendors that have not been functionally tested may contain tetracyclines that affect the maximum expression in Tet-Off systems, or generate background in Tet-On systems. Data here shows induction of luciferase expression from our CHO-AA8-Luc Tet-Off Control Cell Line using different sources of FBS.

631101 Tet System Approved FBS, US-Sourced 500 mL USD $875.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. This FBS has been processed in the United States.

Documents Components Image Data

Back

Fetal bovine serum (FBS) approved for use with tetracycline-inducible expression systems

Fetal bovine serum (FBS) approved for use with tetracycline-inducible expression systems

Fetal bovine serum (FBS) approved for use with tetracycline inducible expression systems. Only Takara Bio USA functionally tests FBS to be sure that no contaminating tetracycline derivatives interfere with induced expression levels.

Back

Our fetal bovine serum is functionally tested to guarantee consistent results with tetracycline-inducible expression systems

Our fetal bovine serum is functionally tested to guarantee consistent results with tetracycline-inducible expression systems

Fetal bovine serum from Clontech is functionally tested to guarantee consistent results with tetracycline-inducible expression systems. Serum from other vendors that have not been functionally tested may contain tetracyclines that affect the maximum expression in Tet-Off systems, or generate background in Tet-On systems. Data here shows induction of luciferase expression from our CHO-AA8-Luc Tet-Off Control Cell Line using different sources of FBS.

631166 Tet-On® 3G Inducible Expression System (Bicistronic Version) Each USD $993.00

License Statement

ID Number  
63 Use of this product is covered by one or more of the following U.S. Patent Nos. and corresponding patent claims outside the U.S.: 6,998,115; 7,427,394. This product is intended for research purposes only. It may not be used for (i) any human or veterinary use, including without limitation therapeutic and prophylactic use, (ii) any clinical use, including without limitation diagnostic use, (iii) screening of chemical and/or biological compounds for the identification of pharmaceutically active agents (including but not limited to screening of small molecules), target validation, preclinical testing services, or drug development. Any use of this product for any of the above mentioned purposes requires a license from the Massachusetts Institute of Technology.
42 Use of the Tetracycline controllable expression systems (the "Tet Technology") is covered by a series of patents including U.S. Patent # 7541446, # 8383364, # 9181556 , European patents EP # 1200607, # 1954811, #2352833 and corresponding patent claims outside these regions which are proprietary to TET Systems GmbH & Co. KG. Academic research institutions are granted an automatic license with the purchase of this product to use the Tet Technology only for internal, academic research purposes, which license specifically excludes the right to sell, or otherwise transfer, the Tet Technology or its component parts to third parties. Notwithstanding the above, academic and not-for profit research institutions whose research using the Tet Technology is sponsored by for profit organizations, which shall receive ownership to any data and results stemming from the sponsored research, shall need a commercial license agreement from TET Systems in order to use the Tet Technology. In accepting this license, all users acknowledge that the Tet Technology is experimental in nature. TET Systems GmbH & Co. KG makes no warranties, express or implied or of any kind, and hereby disclaims any warranties, representations, or guarantees of any kind as to the Tet Technology, patents, or products. All others are invited to request a license from TET Systems GmbH & Co. KG prior to purchasing these reagents or using them for any purpose. Takara Bio USA, Inc. is required by its licensing agreement to submit a report of all purchasers of the Tet-controllable expression system to TET Systems.

For license information, please contact:
GSF/CEO
TET Systems GmbH & Co. KG,
Im Neuenheimer Feld 582
69120 Heidelberg
Germany
Tel: +49 6221 5880400
Fax: +49 6221 5880404
email: info@tetsystems.com
or use the electronic licensing request form via http://www.tetsystems.com/ip-licensing/licensing/for-profit-research
*

The Tet-On 3G Inducible Expression System is a powerful, tightly regulated, tetracycline-inducible mammalian expression system that is controlled by the addition of doxycycline to the culture medium. The Bicistronic Version allows the simultaneous expression of two genes of interest. The system includes a vector set, our highly efficient transfection reagent, Xfect, and Tet System Approved FBS.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Back

The Tet-On 3G systems allow inducible gene expression only in the presence of doxycycline (Dox)

The Tet-On 3G systems allow inducible gene expression only in the presence of doxycycline (Dox)

The Tet-On 3G systems allow inducible gene expression only in the presence of doxycycline (Dox). When Dox binds, the transactivator undergoes a conformational change allowing it to bind tet operator (tetO) repeats within the TRE3G promoter.

Back

The Tet-On 3G system is a highly sensitive tetracycline inducible expression system

The Tet-On 3G system is a highly sensitive tetracycline inducible expression system

The Tet-On 3G system is a highly sensitive tetracycline inducible expression system. Following cotransient transfection of pCMV-Tet3G and pTRE3G-Luc in HeLa cells, increasing levels of Dox were added and expression of luciferase was measured using an anti-luciferase antibody (Panel A) and a luciferase assay (Panel B). Induced expression was very high even with Dox concentrations as low as 10 ng/ml, and was detectable even at 0.1 ng/ml Dox.

Back

The Tet-On 3G system results in the lowest background and as a result provides a much higher fold induction than previous generations of the Tet-On tetracycline inducible expression system

The Tet-On 3G system results in the lowest background and as a result provides a much higher fold induction than previous generations of the Tet-On tetracycline inducible expression system

Tet-On 3G results in the lowest background and as a result provides a much higher fold-induction than previous generations of the Tet-On tetracycline-inducible expression system.

Back

The Tet-On 3G system demonstrates higher sensitivity to doxycycline than Tet-On Advanced system

The Tet-On 3G system demonstrates higher sensitivity to doxycycline than Tet-On Advanced system

The Tet-On 3G system demonstrates higher sensitivity to doxycycline than Tet-On Advanced system. Tet-On 3G and Tet-On Advanced genes were integrated at the same locus in a stable HLF33 cell line expressing luciferase from a TRE promoter. For each of these two double-stable cell lines, induced luciferase expression was measured in response to a range of doxycycline (Dox) concentrations. At 5–10 ng/ml Dox, induced expression was 100– to 150-fold higher for the Tet-On 3G cell line, and at 50 ng/ml, expression was 4.6-fold higher (data kindly provided by Professor W. Hillen, and Dr. C. Berens, University of Erlangen). .

Back

The PTRE3G promoter results in significantly reduced basal expression

The PTRE3G promoter results in significantly reduced basal expression

The PTRE3G promoter results in significantly reduced basal expression. HEK 293 cells were transiently cotranstected with both the response vectors (containing luciferase) and regulator vectors from each of the Tet-On 3G and Tet-On advanced inducible expression systems. The cells were cultured in the presence and absence of Dox, and after 24 hr, luciferase expression was measured. Although both systems provided strong expression in the presence of Dox, the Tet-On 3G system produces far lower background expression in the absence of Dox (inset).

Back

Tet-On 3G inducible expression systems with CMV regulator vectors

Tet-On 3G inducible expression systems with CMV regulator vectors
Tet-On 3G inducible expression systems with CMV regulator vectors.

Required Products

Cat. # Product Size Price License Quantity Details
631106 Tet System Approved FBS 500 mL USD $865.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or its derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems.

Documents Components Image Data

Back

Fetal bovine serum (FBS) approved for use with tetracycline-inducible expression systems

Fetal bovine serum (FBS) approved for use with tetracycline-inducible expression systems

Fetal bovine serum (FBS) approved for use with tetracycline inducible expression systems. Only Takara Bio USA functionally tests FBS to be sure that no contaminating tetracycline derivatives interfere with induced expression levels.

Back

Our fetal bovine serum is functionally tested to guarantee consistent results with tetracycline-inducible expression systems

Our fetal bovine serum is functionally tested to guarantee consistent results with tetracycline-inducible expression systems

Fetal bovine serum from Clontech is functionally tested to guarantee consistent results with tetracycline-inducible expression systems. Serum from other vendors that have not been functionally tested may contain tetracyclines that affect the maximum expression in Tet-Off systems, or generate background in Tet-On systems. Data here shows induction of luciferase expression from our CHO-AA8-Luc Tet-Off Control Cell Line using different sources of FBS.

631101 Tet System Approved FBS, US-Sourced 500 mL USD $875.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. This FBS has been processed in the United States.

Documents Components Image Data

Back

Fetal bovine serum (FBS) approved for use with tetracycline-inducible expression systems

Fetal bovine serum (FBS) approved for use with tetracycline-inducible expression systems

Fetal bovine serum (FBS) approved for use with tetracycline inducible expression systems. Only Takara Bio USA functionally tests FBS to be sure that no contaminating tetracycline derivatives interfere with induced expression levels.

Back

Our fetal bovine serum is functionally tested to guarantee consistent results with tetracycline-inducible expression systems

Our fetal bovine serum is functionally tested to guarantee consistent results with tetracycline-inducible expression systems

Fetal bovine serum from Clontech is functionally tested to guarantee consistent results with tetracycline-inducible expression systems. Serum from other vendors that have not been functionally tested may contain tetracyclines that affect the maximum expression in Tet-Off systems, or generate background in Tet-On systems. Data here shows induction of luciferase expression from our CHO-AA8-Luc Tet-Off Control Cell Line using different sources of FBS.

631165 Tet-On® 3G Inducible Expression System (with mCherry) Each USD $1095.00

License Statement

ID Number  
44 The DsRed-Monomer and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. Patents: 7,005,511; 7,157,566; 7,393,923 and 7,250,298.
63 Use of this product is covered by one or more of the following U.S. Patent Nos. and corresponding patent claims outside the U.S.: 6,998,115; 7,427,394. This product is intended for research purposes only. It may not be used for (i) any human or veterinary use, including without limitation therapeutic and prophylactic use, (ii) any clinical use, including without limitation diagnostic use, (iii) screening of chemical and/or biological compounds for the identification of pharmaceutically active agents (including but not limited to screening of small molecules), target validation, preclinical testing services, or drug development. Any use of this product for any of the above mentioned purposes requires a license from the Massachusetts Institute of Technology.
72 Living Colors Fluorescent Protein Products:

Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Customer Service. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Under the Non-Commercial License, Takara Bio USA, Inc. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

For-Profit Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

Not-For-Profit Non-Commercial Use License:
A copy of the Tet-On® 3G Inducible Expression System (with mCherry) product License Agreement can be found by clicking here.
42 Use of the Tetracycline controllable expression systems (the "Tet Technology") is covered by a series of patents including U.S. Patent # 7541446, # 8383364, # 9181556 , European patents EP # 1200607, # 1954811, #2352833 and corresponding patent claims outside these regions which are proprietary to TET Systems GmbH & Co. KG. Academic research institutions are granted an automatic license with the purchase of this product to use the Tet Technology only for internal, academic research purposes, which license specifically excludes the right to sell, or otherwise transfer, the Tet Technology or its component parts to third parties. Notwithstanding the above, academic and not-for profit research institutions whose research using the Tet Technology is sponsored by for profit organizations, which shall receive ownership to any data and results stemming from the sponsored research, shall need a commercial license agreement from TET Systems in order to use the Tet Technology. In accepting this license, all users acknowledge that the Tet Technology is experimental in nature. TET Systems GmbH & Co. KG makes no warranties, express or implied or of any kind, and hereby disclaims any warranties, representations, or guarantees of any kind as to the Tet Technology, patents, or products. All others are invited to request a license from TET Systems GmbH & Co. KG prior to purchasing these reagents or using them for any purpose. Takara Bio USA, Inc. is required by its licensing agreement to submit a report of all purchasers of the Tet-controllable expression system to TET Systems.

For license information, please contact:
GSF/CEO
TET Systems GmbH & Co. KG,
Im Neuenheimer Feld 582
69120 Heidelberg
Germany
Tel: +49 6221 5880400
Fax: +49 6221 5880404
email: info@tetsystems.com
or use the electronic licensing request form via http://www.tetsystems.com/ip-licensing/licensing/for-profit-research
*

The Tet-On 3G Inducible Expression System (with mCherry) is a powerful, tightly regulated, tetracycline-inducible mammalian expression system that is controlled by the addition of doxycycline to the culture medium. It allows the simultaneous expression of a gene of interest and a red fluorescent protein marker. The system includes all necessary vectors, our highly efficient transfection reagent, Xfect, and Tet System Approved FBS.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Back

The Tet-On 3G systems allow inducible gene expression only in the presence of doxycycline (Dox)

The Tet-On 3G systems allow inducible gene expression only in the presence of doxycycline (Dox)

The Tet-On 3G systems allow inducible gene expression only in the presence of doxycycline (Dox). When Dox binds, the transactivator undergoes a conformational change allowing it to bind tet operator (tetO) repeats within the TRE3G promoter.

Back

The Tet-On 3G system is a highly sensitive tetracycline inducible expression system

The Tet-On 3G system is a highly sensitive tetracycline inducible expression system

The Tet-On 3G system is a highly sensitive tetracycline inducible expression system. Following cotransient transfection of pCMV-Tet3G and pTRE3G-Luc in HeLa cells, increasing levels of Dox were added and expression of luciferase was measured using an anti-luciferase antibody (Panel A) and a luciferase assay (Panel B). Induced expression was very high even with Dox concentrations as low as 10 ng/ml, and was detectable even at 0.1 ng/ml Dox.

Back

The Tet-On 3G system results in the lowest background and as a result provides a much higher fold induction than previous generations of the Tet-On tetracycline inducible expression system

The Tet-On 3G system results in the lowest background and as a result provides a much higher fold induction than previous generations of the Tet-On tetracycline inducible expression system

Tet-On 3G results in the lowest background and as a result provides a much higher fold-induction than previous generations of the Tet-On tetracycline-inducible expression system.

Back

The Tet-On 3G system demonstrates higher sensitivity to doxycycline than Tet-On Advanced system

The Tet-On 3G system demonstrates higher sensitivity to doxycycline than Tet-On Advanced system

The Tet-On 3G system demonstrates higher sensitivity to doxycycline than Tet-On Advanced system. Tet-On 3G and Tet-On Advanced genes were integrated at the same locus in a stable HLF33 cell line expressing luciferase from a TRE promoter. For each of these two double-stable cell lines, induced luciferase expression was measured in response to a range of doxycycline (Dox) concentrations. At 5–10 ng/ml Dox, induced expression was 100– to 150-fold higher for the Tet-On 3G cell line, and at 50 ng/ml, expression was 4.6-fold higher (data kindly provided by Professor W. Hillen, and Dr. C. Berens, University of Erlangen). .

Back

The PTRE3G promoter results in significantly reduced basal expression

The PTRE3G promoter results in significantly reduced basal expression

The PTRE3G promoter results in significantly reduced basal expression. HEK 293 cells were transiently cotranstected with both the response vectors (containing luciferase) and regulator vectors from each of the Tet-On 3G and Tet-On advanced inducible expression systems. The cells were cultured in the presence and absence of Dox, and after 24 hr, luciferase expression was measured. Although both systems provided strong expression in the presence of Dox, the Tet-On 3G system produces far lower background expression in the absence of Dox (inset).

Back

Three generations of Tet-On System vectors

Three generations of Tet-On System vectors
Three generations of Tet-On System vectors. The Tet-On 3G inducible expression system offers a significant improvement over Tet-On and Tet-On Advanced by further reducing basal expression and increasing sensitivity to doxycycline.

Back

Tet-On 3G inducible expression systems with CMV regulator vectors

Tet-On 3G inducible expression systems with CMV regulator vectors
Tet-On 3G inducible expression systems with CMV regulator vectors.

Required Products

Cat. # Product Size Price License Quantity Details
631106 Tet System Approved FBS 500 mL USD $865.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or its derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems.

Documents Components Image Data

Back

Fetal bovine serum (FBS) approved for use with tetracycline-inducible expression systems

Fetal bovine serum (FBS) approved for use with tetracycline-inducible expression systems

Fetal bovine serum (FBS) approved for use with tetracycline inducible expression systems. Only Takara Bio USA functionally tests FBS to be sure that no contaminating tetracycline derivatives interfere with induced expression levels.

Back

Our fetal bovine serum is functionally tested to guarantee consistent results with tetracycline-inducible expression systems

Our fetal bovine serum is functionally tested to guarantee consistent results with tetracycline-inducible expression systems

Fetal bovine serum from Clontech is functionally tested to guarantee consistent results with tetracycline-inducible expression systems. Serum from other vendors that have not been functionally tested may contain tetracyclines that affect the maximum expression in Tet-Off systems, or generate background in Tet-On systems. Data here shows induction of luciferase expression from our CHO-AA8-Luc Tet-Off Control Cell Line using different sources of FBS.

631101 Tet System Approved FBS, US-Sourced 500 mL USD $875.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. This FBS has been processed in the United States.

Documents Components Image Data

Back

Fetal bovine serum (FBS) approved for use with tetracycline-inducible expression systems

Fetal bovine serum (FBS) approved for use with tetracycline-inducible expression systems

Fetal bovine serum (FBS) approved for use with tetracycline inducible expression systems. Only Takara Bio USA functionally tests FBS to be sure that no contaminating tetracycline derivatives interfere with induced expression levels.

Back

Our fetal bovine serum is functionally tested to guarantee consistent results with tetracycline-inducible expression systems

Our fetal bovine serum is functionally tested to guarantee consistent results with tetracycline-inducible expression systems

Fetal bovine serum from Clontech is functionally tested to guarantee consistent results with tetracycline-inducible expression systems. Serum from other vendors that have not been functionally tested may contain tetracyclines that affect the maximum expression in Tet-Off systems, or generate background in Tet-On systems. Data here shows induction of luciferase expression from our CHO-AA8-Luc Tet-Off Control Cell Line using different sources of FBS.

631164 Tet-On® 3G Inducible Expression System (with ZsGreen1) Each USD $1095.00

License Statement

ID Number  
63 Use of this product is covered by one or more of the following U.S. Patent Nos. and corresponding patent claims outside the U.S.: 6,998,115; 7,427,394. This product is intended for research purposes only. It may not be used for (i) any human or veterinary use, including without limitation therapeutic and prophylactic use, (ii) any clinical use, including without limitation diagnostic use, (iii) screening of chemical and/or biological compounds for the identification of pharmaceutically active agents (including but not limited to screening of small molecules), target validation, preclinical testing services, or drug development. Any use of this product for any of the above mentioned purposes requires a license from the Massachusetts Institute of Technology.
42 Use of the Tetracycline controllable expression systems (the "Tet Technology") is covered by a series of patents including U.S. Patent # 7541446, # 8383364, # 9181556 , European patents EP # 1200607, # 1954811, #2352833 and corresponding patent claims outside these regions which are proprietary to TET Systems GmbH & Co. KG. Academic research institutions are granted an automatic license with the purchase of this product to use the Tet Technology only for internal, academic research purposes, which license specifically excludes the right to sell, or otherwise transfer, the Tet Technology or its component parts to third parties. Notwithstanding the above, academic and not-for profit research institutions whose research using the Tet Technology is sponsored by for profit organizations, which shall receive ownership to any data and results stemming from the sponsored research, shall need a commercial license agreement from TET Systems in order to use the Tet Technology. In accepting this license, all users acknowledge that the Tet Technology is experimental in nature. TET Systems GmbH & Co. KG makes no warranties, express or implied or of any kind, and hereby disclaims any warranties, representations, or guarantees of any kind as to the Tet Technology, patents, or products. All others are invited to request a license from TET Systems GmbH & Co. KG prior to purchasing these reagents or using them for any purpose. Takara Bio USA, Inc. is required by its licensing agreement to submit a report of all purchasers of the Tet-controllable expression system to TET Systems.

For license information, please contact:
GSF/CEO
TET Systems GmbH & Co. KG,
Im Neuenheimer Feld 582
69120 Heidelberg
Germany
Tel: +49 6221 5880400
Fax: +49 6221 5880404
email: info@tetsystems.com
or use the electronic licensing request form via http://www.tetsystems.com/ip-licensing/licensing/for-profit-research
*

The Tet-On 3G Inducible Expression System is a powerful, tightly regulated, tetracycline-inducible mammalian expression system that is controlled by the addition of doxycycline to the culture medium. The Tet-On 3G Vector Set (with ZsGreen1) allows the simultaneous expression of a gene of interest and a green fluorescent protein marker. The system includes a vector set, our highly efficient transfection reagent, Xfect, and Tet System Approved FBS.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Back

The Tet-On 3G systems allow inducible gene expression only in the presence of doxycycline (Dox)

The Tet-On 3G systems allow inducible gene expression only in the presence of doxycycline (Dox)

The Tet-On 3G systems allow inducible gene expression only in the presence of doxycycline (Dox). When Dox binds, the transactivator undergoes a conformational change allowing it to bind tet operator (tetO) repeats within the TRE3G promoter.

Back

The Tet-On 3G system is a highly sensitive tetracycline inducible expression system

The Tet-On 3G system is a highly sensitive tetracycline inducible expression system

The Tet-On 3G system is a highly sensitive tetracycline inducible expression system. Following cotransient transfection of pCMV-Tet3G and pTRE3G-Luc in HeLa cells, increasing levels of Dox were added and expression of luciferase was measured using an anti-luciferase antibody (Panel A) and a luciferase assay (Panel B). Induced expression was very high even with Dox concentrations as low as 10 ng/ml, and was detectable even at 0.1 ng/ml Dox.

Back

The Tet-On 3G system results in the lowest background and as a result provides a much higher fold induction than previous generations of the Tet-On tetracycline inducible expression system

The Tet-On 3G system results in the lowest background and as a result provides a much higher fold induction than previous generations of the Tet-On tetracycline inducible expression system

Tet-On 3G results in the lowest background and as a result provides a much higher fold-induction than previous generations of the Tet-On tetracycline-inducible expression system.

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The Tet-On 3G system demonstrates higher sensitivity to doxycycline than Tet-On Advanced system

The Tet-On 3G system demonstrates higher sensitivity to doxycycline than Tet-On Advanced system

The Tet-On 3G system demonstrates higher sensitivity to doxycycline than Tet-On Advanced system. Tet-On 3G and Tet-On Advanced genes were integrated at the same locus in a stable HLF33 cell line expressing luciferase from a TRE promoter. For each of these two double-stable cell lines, induced luciferase expression was measured in response to a range of doxycycline (Dox) concentrations. At 5–10 ng/ml Dox, induced expression was 100– to 150-fold higher for the Tet-On 3G cell line, and at 50 ng/ml, expression was 4.6-fold higher (data kindly provided by Professor W. Hillen, and Dr. C. Berens, University of Erlangen). .

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The PTRE3G promoter results in significantly reduced basal expression

The PTRE3G promoter results in significantly reduced basal expression

The PTRE3G promoter results in significantly reduced basal expression. HEK 293 cells were transiently cotranstected with both the response vectors (containing luciferase) and regulator vectors from each of the Tet-On 3G and Tet-On advanced inducible expression systems. The cells were cultured in the presence and absence of Dox, and after 24 hr, luciferase expression was measured. Although both systems provided strong expression in the presence of Dox, the Tet-On 3G system produces far lower background expression in the absence of Dox (inset).

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Tet-On 3G inducible expression systems with CMV regulator vectors

Tet-On 3G inducible expression systems with CMV regulator vectors
Tet-On 3G inducible expression systems with CMV regulator vectors.

Required Products

Cat. # Product Size Price License Quantity Details
631106 Tet System Approved FBS 500 mL USD $865.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or its derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems.

Documents Components Image Data

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Fetal bovine serum (FBS) approved for use with tetracycline-inducible expression systems

Fetal bovine serum (FBS) approved for use with tetracycline-inducible expression systems

Fetal bovine serum (FBS) approved for use with tetracycline inducible expression systems. Only Takara Bio USA functionally tests FBS to be sure that no contaminating tetracycline derivatives interfere with induced expression levels.

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Our fetal bovine serum is functionally tested to guarantee consistent results with tetracycline-inducible expression systems

Our fetal bovine serum is functionally tested to guarantee consistent results with tetracycline-inducible expression systems

Fetal bovine serum from Clontech is functionally tested to guarantee consistent results with tetracycline-inducible expression systems. Serum from other vendors that have not been functionally tested may contain tetracyclines that affect the maximum expression in Tet-Off systems, or generate background in Tet-On systems. Data here shows induction of luciferase expression from our CHO-AA8-Luc Tet-Off Control Cell Line using different sources of FBS.

631101 Tet System Approved FBS, US-Sourced 500 mL USD $875.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. This FBS has been processed in the United States.

Documents Components Image Data

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Fetal bovine serum (FBS) approved for use with tetracycline-inducible expression systems

Fetal bovine serum (FBS) approved for use with tetracycline-inducible expression systems

Fetal bovine serum (FBS) approved for use with tetracycline inducible expression systems. Only Takara Bio USA functionally tests FBS to be sure that no contaminating tetracycline derivatives interfere with induced expression levels.

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Our fetal bovine serum is functionally tested to guarantee consistent results with tetracycline-inducible expression systems

Our fetal bovine serum is functionally tested to guarantee consistent results with tetracycline-inducible expression systems

Fetal bovine serum from Clontech is functionally tested to guarantee consistent results with tetracycline-inducible expression systems. Serum from other vendors that have not been functionally tested may contain tetracyclines that affect the maximum expression in Tet-Off systems, or generate background in Tet-On systems. Data here shows induction of luciferase expression from our CHO-AA8-Luc Tet-Off Control Cell Line using different sources of FBS.

*You must be logged in to a Business Account in order to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@takarabio.com.

FBS for Tet system Tet-approved FBS
Tips and FAQs Learn about Tet systems
Selection guides Tet-On 3G kit options

Overview

  • Lowest background and highest sensitivity
  • Highest fold induction
  • 14 kit combinations—click here for available options
  • Monitor induced expression using fluorescent proteins and a bidirectional promoter
  • Ready-made Tet-On 3G cell lines cut your time in half
  • Retroviral (Retro-X), lentiviral (Lenti-X) and adenoviral (Adeno-X) formats are available

More Information

Applications

  • Inducible expression in mammalian cells
  • Inducible expression in stem cells and hematopoietic cells (EF1-alpha promoter version)
  • Co-inducible expression of two genes of interest (bicistronic version)
  • Co-express a fluorescent protein (mCherry and ZsGreen1 versions)

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


Tet-One tetracycline-inducible expression system, technology overview

Tighly controlled expression system that is as easy to use as any constitutive system

Until now, the best inducible mammalian expression systems have all required a two-vector format. Tet-One inducible expression systems are the only commercially available single-vector systems that can deliver a >10,000-fold difference in expression levels between induced and uninduced states.

Tet-One technology overview All inducible systems

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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