- Cellartis Cardiomyocytes (from ChiPSC22) Kit (Takara Bio, Cat. # Y10075)
- Cellartis Cardiomyocytes (from ChiPSC22)
- Cellartis CM Thawing Base
- Cellartis CM Culture Base
- Fetal Bovine Serum (FBS) (Fisher Scientific, Cat. # 16140)
- Fibronectin (Sigma-Aldrich, Cat. # F0895)
- 384-well plate (Corning Costar, Cat. # 3712)
- MicroClime lid (Labcyte, Cat.# LLS-0310)
- PBS Dulbecco's with Ca2+ & Mg2+ (D-PBS +/+)
- FLIPR Calcium 5 Assay Kit (Molecular Devices, Cat # R8185, R8186, or R8187)
- FLIPR Tetra High-Throughput Cellular Screening System (Molecular Devices)
- General cell culture equipment used in cell culture laboratory
- Technical notes
- Cardiomyocytes in FLIPR 384-well plate format
- Cardiomyocytes on the Patchliner system
- Cardiomyocytes on the Maestro MEA system
- Cardiomyocytes on the MED64 MEA system
- Cardiomyocytes on the CardioExcyte 96 system
- Cardiomyocytes on the xCELLigence RTCA CardioECR system
- Reprogramming fibroblasts
- Reprogramming PBMCs
- Spin embryoid body formation
- Transferring iPSCs from other media to DEF-CS
- Transferring iPSCs on MEFs to DEF-CS
- Selection guides
STEM CELL APPLICATION PROTOCOL
Cellartis cardiomyocytes in FLIPR 384-well plate format
Cellartis cardiomyocytes are derived from human induced pluripotent stem cells and provide a promising physiologically-relevant, human model for preclinical safety evaluation and drug screening. The FLIPR Tetra High-Throughput Cellular Screening System is a real-time kinetic cellular assay screening system that allows for detection of intracellular Ca2+ flux and cardiac beating in a high-throughput format. Cellartis cardiomyocytes used in combination with this technology demonstrate the potential to predict cardiotoxic responses accurately and to screen compound efficacy.
A highly homogeneous population of cardiomyocytes derived from human induced pluripotent stem cells.
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