Improve limit of detection for low copy number targets
Preamplification is a powerful technique to boost the copy number of targets of interest, allowing the identification of rare or low-level targets or pathogens in complex samples. The key consideration when performing preamplification is ensuring your mix is highly sensitive and minimizes bias.
To maximize PCR efficiency for all targets in a preamplification reaction, we developed Prelude PreAmp Master Mix.
- Optimized polymerase and buffer enable the highest level of unbiased preamplification of >100 targets from as little as 10 pg of cDNA or gDNA
- In-house comparison data show superior preamplification of 162 breast cancer-related genes from FFPE tissue
- Our data show the least-biased preamplification of any commercially available preamp mixes, leading to at least 2–3X fewer outliers
- Our one-step version combines Prelude PreAmp Master Mix with PrimeScript reverse transcriptase to streamline workflows starting with limited amounts of RNA
Detect more without bias using Prelude PreAmp Master Mix
Comparison studies involving 20 ng of cDNA that had not been preamplified versus 100 pg of cDNA that was subjected to 10 cycles of preamplification using Prelude PreAmp Master Mix clearly indicate comparable amounts of cDNA and gene expression levels. (Figure 1).
Figure 1. Uniform representation following preamplification. 10 cycles of preamplification using Prelude PreAmp Master Mix can make 100 pg of starting cDNA detectable using qPCR without introducing bias and maintaining uniform representation. The above graph displays data for all 96 target genes in the PrimerArray Cell Cycle (Human) panel, but only some of the gene names are shown on the x-axis to ensure their readability. Review the full comparison study, including Prelude PreAmp mix’s performance on breast cancer targets in FFPE tissue »
Achieve the most unbiased, target-specific preamplification from limited samples using Prelude PreAmp Master Mix
View data on sensitive, unbiased preamplification from limited samples.
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