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Lyophilized high-performing enzymes for enhanced stability
Designing and developing clinical assays is often a complicated process with numerous variables. On top of the critical needs for accuracy, sensitivity, and sample reproducibility are requirements for stability and consistency during shipping and storage across testing sites and regions. Stability during shipping and storage can be especially important due to freezer storage availability or costs associated with shipping on dry ice. Lyophilization of polymerases, mixes, and complete molecular assays addresses some of these challenging requirements and can lower some of the barriers of entry into new and emerging markets that don't have significant storage at –20°C or below.
However, many conventional enzyme formats are incompatible with lyophilization—primarily owing to the presence of high concentrations of glycerol in the buffer. While glycerol is an excellent preservative of liquids, it is highly resistant to freeze-drying protocols required to produce a lyophilized product. As such, applications necessitating either low-glycerol or glycerol-free enzymes and mixes, such as point-of-care-testing, demand specialized formulations to be successful.
At Takara Bio, we don’t just supply great enzymes, we supply them in a format that works for your assay requirements. We have capabilities to provide low-glycerol and glycerol-free versions of high-performing reverse transcriptases and polymerases. We can quickly provide samples of these enzymes in lyo-ready liquid formats or already lyophilized strips. Final products can be custom packaged in tubes, bottles, strips, or plates and produced with custom assays premixed.
|Sample Cat. #||Product Name||Recommended Application|
|XA0260||PrimeScript III Reverse Transcriptase (3,000 U/µl, 5% Glycerol)||cDNA synthesis for qPCR|
|XA0261||SpeedSTAR HS DNA Polymerase (25 U/µl, Glycerol-Free)||Amplification of large DNA fragments and fast PCR|
|XA0262||Recombinant RNase Inhibitor (300 U/µl, 5% Glycerol)||cDNA synthesis and in vitro applications such as transcription and translation|
|XA0259||PrimeSTAR GXL DNA Polymerase (25 U/µl, Glycerol-Free)||High-fidelity PCR and amplification of cDNA libraries|
|639589||SMART MMLV Reverse Transcriptase (25 U/μl, Glycerol-Free)||Synthesis of long, full-length cDNA|
|XA0263||TaKaRa Taq HS (25 U/µl, Glycerol-Free)||Economical routine PCR|
|XA0264||TaKaRa Ex Taq HS (30 U/µl, Glycerol-Free)||Routine PCR or probe-based qPCR from diverse sample types|
Table I. Ready-to-ship samples of some of our best-selling enzymes in lyo-ready liquid or already lyophilized formats.
Learn more about some of our most sought-after enzymes for use in clinical development or request more information to try a sample by completing the form on the left or using the contact us page.
Need a sample of one of our enzymes?
You can request a sample of one of our high-concentration or glycerol-free enzymes by contacting us. We have ready-to-ship samples of many of our catalog products. Alternatively, you can request a sample by contacting your local Territory Manager.Request a sample Contact us
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