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Reference RNA for real-time qPCR

Universal reference total RNA is control total RNA derived from whole-tissue sources. Each species-specific reference total RNA is made by pooling the total RNA extracts from a collection of different tissues, thus providing the broadest coverage of expressed genes. With each of reference total RNA sample, you can easily compare microarray or real-time PCR data generated from different experiments. For microarray experiments, simply hybridize a reference total RNA probe to a microarray each time you perform an experiment, and then use the control signal to normalize your results. We provide enough reference total RNA for up to 80 microarray experiments depending on the labeling technique. Since these RNA mixes are prepared on an industrial scale, there is minimal lot-to-lot variability. Our reference total RNA is the best approach for building gene expression databases to compare expression profiles from different tissues or cell lines.

Universal reference total RNA is control total RNA derived from whole-tissue sources. Each species-specific reference total RNA is made by pooling the total RNA extracts from a collection of different tissues, thus providing the broadest coverage of expressed genes. With each of reference total RNA sample, you can easily compare microarray or real-time PCR data generated from different experiments. For microarray experiments, simply hybridize a reference total RNA probe to a microarray each time you perform an experiment, and then use the control signal to normalize your results. We provide enough reference total RNA for up to 80 microarray experiments depending on the labeling technique. Since these RNA mixes are prepared on an industrial scale, there is minimal lot-to-lot variability. Our reference total RNA is the best approach for building gene expression databases to compare expression profiles from different tissues or cell lines.

Additionally, the qPCR Human Reference Total RNA can be used in gene expression analysis to compare data from a variety of qPCR experiments. qPCR Human Reference Total RNA is prepared by pooling the total RNA from a collection of different human tissues, so it provides broader gene coverage than reference RNA mixtures prepared from cell lines.

Optimized gene representation

In developing these RNA standards, we compared results for a number of different tissue combinations to identify the most representative mix. In comparison to a competitor’s RNA standard made from cell lines, we found that using pooled RNA extracted from whole tissues provided better gene representation and more homogenous signal intensities across all genes. Additionally, pooled-tissue RNA samples have very stable expression profiles, providing a high level of reproducibility between different lots. As a result, our reference total RNA consistently outperforms the competition in terms of gene coverage and hybridization consistency.

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Cat. # Product Size License Quantity Details
636690 qPCR Human Reference Total RNA 25 ug USD $267.00

A mixture of total RNAs from a collection of adult human tissues, chosen to represent a broad range of expressed genes. Both male and female donors are represented.

qPCR Reference Total RNA serves as a standard for the accurate and reproducible comparison of gene expression data using real-time quantitative PCR (qPCR). It may also be used as a source of positive control templates for validating qPCR primer designs.

Total RNA was isolated by a modified guanidinium thiocyanate method.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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High-, medium- and low-abundance gene targets are easily detected in qPCR Human Reference cDNA

High-, medium- and low-abundance gene targets are easily detected in qPCR Human Reference cDNA
High-, medium- and low-abundance gene targets are easily detected in qPCR Human Reference cDNA. Oligo dT-primed (Panels A–C) and random oligo-primed (Panels D–F) qPCR Human Reference cDNA samples were each serially diluted fivefold such that the final quantities of cDNA template in the qPCR reactions were 20 ng, 4 ng, 800 pg, 160 pg, 32 pg,and 6.4 pg. Data were obtained on a Stratagene Mx3000P real-time PCR instrument. The primer sets for all three genes demonstrate the ability to detect a wide range of signals from the serially diluted samples. PPIA = peptidylprolyl isomerase A (cyclophilin A; high-abundance); HPRT = hypoxanthine phosphoribosyltransferase I (medium-abundance); PBGD = porphobilinogen deaminase (low-abundance). Slope and R2 values refer to the line determinedby plotting Ct values versus template quantity

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636690: qPCR Human Reference Total RNA

636690: qPCR Human Reference Total RNA
636658 Rat Universal Reference Total RNA 2 x 200 ug USD $733.00

Mixture of total RNAs from a collection of adult rat tissues, chosen to represent a broad range
of expressed genes. Both male and female animals are represented. Rat Universal Reference Total RNA serves as a standard for the comparison of gene expression data and is useful as an internal control for microarray expression profiling experiments.

Total RNA was isolated by a modified guanidinium thiocyanate method.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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Clontech Rat Universal Reference Total RNA demonstrates more than 90% gene coverage

Clontech Rat Universal Reference Total RNA demonstrates more than 90% gene coverage
Clontech Rat Universal Reference Total RNA demonstrates more than 90% gene coverage. Cy3 labeled probes were generated using our Rat Reference Total RNA (Row A), another vendor’s reference total RNA (Row B), a random RNA mixture (Row C), and an individual RNA sample (Row D). Probes were hybridized to a glass rat array containing 3,766 long oligonucleotides. Expression results were analyzed using GeneSpring software (version 5.0) to cluster genes according to their expression patterns. Varying colors reflect the ratio of the intensity of any gene on each array to its median intensity across all arrays. The red and blue colors reflect high and low ratios, respectively. Our results indicate that the Reference Total RNA has more than 90% gene coverage with even distribution and outperforms another vendor’s universal RNA mixture.

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636658: Rat Universal Reference Total RNA

636658: Rat Universal Reference Total RNA
636657 Mouse Universal Reference Total RNA 2 x 200 ug USD $733.00

Mixture of total RNAs from a collection of adult mouse tissues, chosen to represent a broad range of expressed genes. Both male and female animals are represented. Universal Reference Total RNA serves as a standard for comparing gene expression data and is useful as an internal control for microarray expression profiling experiments.

Total RNA was isolated by a modified guanidinium thiocyanate method.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Clontech Rat Universal Reference Total RNA demonstrates more than 90% gene coverage

Clontech Rat Universal Reference Total RNA demonstrates more than 90% gene coverage
Clontech Rat Universal Reference Total RNA demonstrates more than 90% gene coverage. Cy3 labeled probes were generated using our Rat Reference Total RNA (Row A), another vendor’s reference total RNA (Row B), a random RNA mixture (Row C), and an individual RNA sample (Row D). Probes were hybridized to a glass rat array containing 3,766 long oligonucleotides. Expression results were analyzed using GeneSpring software (version 5.0) to cluster genes according to their expression patterns. Varying colors reflect the ratio of the intensity of any gene on each array to its median intensity across all arrays. The red and blue colors reflect high and low ratios, respectively. Our results indicate that the Reference Total RNA has more than 90% gene coverage with even distribution and outperforms another vendor’s universal RNA mixture.

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636657: Mouse Universal Reference Total RNA

636657: Mouse Universal Reference Total RNA

Overview

  • Featuring the broadest possible gene representation with minimal lot-to-lot variation

  • Use with any microarray or labeling method

  • Less gene-to-gene signal variation

  • Virtually free of genomic DNA

More Information

Applications

  • Microarray analysis

  • Real-time PCR/qPCR

  • RT-PCR

  • Northern analysis

  • Gene-expression analysis

Source

  • Extracted from a mixture of whole tissues, not cultured cells

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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