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RNA-seq

Legacy RNA-seq kits

We offer multiple generations of transcriptome analysis kits. The core SMART (Switching Mechanism at 5' end of RNA Template) technology employed by these legacy kits provides full-length transcript information, enabling analysis of transcript isoforms, gene fusions, point mutations, and more. While our newest generation of kits provide advantages over these legacy kits, we advise finishing ongoing projects with the same kit. For new experiments, we recommend using our most advanced solutions for single-cell RNA-seq or RNA-seq.

Cat. #

Product

Size

License

Quantity

Details

634852

SMARTer® Ultra® Low Input RNA Kit for Sequencing - v3

192 Rxns

Inquire for Quotation

The SMARTer Ultra Low Input RNA Kit for Sequencing - v3 allows direct synthesis of high-quality cDNA when starting with 1–1,000 cells or 10 pg–10 ng of total RNA. The kit has been designed and validated to prepare cDNA samples for library preparation and sequencing using the Ion Torrent or Illumina sequencing platforms. SMART (Switching Mechanism At 5' End of RNA Template) technology enriches for full-length transcripts and maintains the true representation of the original mRNA transcripts; these factors are critical for transcriptome sequencing and gene expression analysis. This kit improves on previous generations of SMARTer Ultra Low kits by simplifying the workflow, identifying more genes, and increasing the representation of GC-rich genes. It is a complete kit that supplies all required reagents, including SeqAmp DNA polymerase, an optimized PCR enzyme that has been verified to perform well with this kit.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

Required Products

Cat. #	Product	Size	Price
639207	Advantage® 2 PCR Kit	30 Rxns	USD $181.00
This kit is based on Takara's Advantage 2 Polymerase Mix. It contains all reagents needed for a hot-start PCR, including control template and primer mix. This kit is ideal for PCR applications that require high fidelity, such as cDNA amplification or library construction. Enough reagents are supplied for 30 PCR reactions of 50 μl each. Documents Components Image Data Back Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix *Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq* polymerase mix.** 5 μl of PCR products were run on a 1.1% agarose/EtBr gel. Lane 1: The 0.4-kb TNFR II fragment is readily obtained with Advantage 2. Lane 2: No product is seen with Taq polymerase. Lane M: DNA size marker Back Amplification of various large templates using Advantage 2 Polymerase Mix Amplification of various large templates using Advantage 2 Polymerase Mix. 1–3 μl of each PCR product was run on a 1.1% agarose/EtBr gel. Lane 1: 2.5-kb E. coli DNA polymerase gene amplified from genomic DNA. Lane 2: 3.5-kb bovine pancreatic trypsin inhibitor gene amplified from calf thymus genomic DNA. Lane 3: 5.9-kb human IL-1β gene amplified from human genomic DNA. Lane 4: 8.5-kb human titin cDNA amplified from a SMART Human Skeletal Muscle cDNA library. Lane 5: 18.5-kb λ insert amplified from a λ clone. Lane M: λ/Hind III DNA size marker. Back 639207: Advantage 2 PCR Kit
638504	SeqAmp™ DNA Polymerase	50 Rxns	USD $147.00
SeqAmp DNA Polymerase is a high-fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Documents Components Image Data Back cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain poly(A)⁺ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back 638504: SeqAmp DNA Polymerase
638509	SeqAmp™ DNA Polymerase	200 Rxns	USD $408.00
SeqAmp DNA Polymerase is a high fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Documents Components Image Data Back 638509: SeqAmp DNA Polymerase

634850

SMARTer® Ultra® Low Input RNA Kit for Sequencing - v3

48 Rxns

USD $4802.00

Notice to purchaser

Components

Required Products

Cat. #	Product	Size	Price
639207	Advantage® 2 PCR Kit	30 Rxns	USD $181.00
This kit is based on Takara's Advantage 2 Polymerase Mix. It contains all reagents needed for a hot-start PCR, including control template and primer mix. This kit is ideal for PCR applications that require high fidelity, such as cDNA amplification or library construction. Enough reagents are supplied for 30 PCR reactions of 50 μl each. Documents Components Image Data Back Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix *Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq* polymerase mix.** 5 μl of PCR products were run on a 1.1% agarose/EtBr gel. Lane 1: The 0.4-kb TNFR II fragment is readily obtained with Advantage 2. Lane 2: No product is seen with Taq polymerase. Lane M: DNA size marker Back Amplification of various large templates using Advantage 2 Polymerase Mix Amplification of various large templates using Advantage 2 Polymerase Mix. 1–3 μl of each PCR product was run on a 1.1% agarose/EtBr gel. Lane 1: 2.5-kb E. coli DNA polymerase gene amplified from genomic DNA. Lane 2: 3.5-kb bovine pancreatic trypsin inhibitor gene amplified from calf thymus genomic DNA. Lane 3: 5.9-kb human IL-1β gene amplified from human genomic DNA. Lane 4: 8.5-kb human titin cDNA amplified from a SMART Human Skeletal Muscle cDNA library. Lane 5: 18.5-kb λ insert amplified from a λ clone. Lane M: λ/Hind III DNA size marker. Back 639207: Advantage 2 PCR Kit
638504	SeqAmp™ DNA Polymerase	50 Rxns	USD $147.00
SeqAmp DNA Polymerase is a high-fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Documents Components Image Data Back cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain poly(A)⁺ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back 638504: SeqAmp DNA Polymerase
638509	SeqAmp™ DNA Polymerase	200 Rxns	USD $408.00
SeqAmp DNA Polymerase is a high fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Documents Components Image Data Back 638509: SeqAmp DNA Polymerase

634848

SMARTer® Ultra® Low Input RNA Kit for Sequencing - v3

12 Rxns

USD $1714.00

Notice to purchaser

Components

Required Products

Cat. #	Product	Size	Price
639207	Advantage® 2 PCR Kit	30 Rxns	USD $181.00
This kit is based on Takara's Advantage 2 Polymerase Mix. It contains all reagents needed for a hot-start PCR, including control template and primer mix. This kit is ideal for PCR applications that require high fidelity, such as cDNA amplification or library construction. Enough reagents are supplied for 30 PCR reactions of 50 μl each. Documents Components Image Data Back Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix *Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq* polymerase mix.** 5 μl of PCR products were run on a 1.1% agarose/EtBr gel. Lane 1: The 0.4-kb TNFR II fragment is readily obtained with Advantage 2. Lane 2: No product is seen with Taq polymerase. Lane M: DNA size marker Back Amplification of various large templates using Advantage 2 Polymerase Mix Amplification of various large templates using Advantage 2 Polymerase Mix. 1–3 μl of each PCR product was run on a 1.1% agarose/EtBr gel. Lane 1: 2.5-kb E. coli DNA polymerase gene amplified from genomic DNA. Lane 2: 3.5-kb bovine pancreatic trypsin inhibitor gene amplified from calf thymus genomic DNA. Lane 3: 5.9-kb human IL-1β gene amplified from human genomic DNA. Lane 4: 8.5-kb human titin cDNA amplified from a SMART Human Skeletal Muscle cDNA library. Lane 5: 18.5-kb λ insert amplified from a λ clone. Lane M: λ/Hind III DNA size marker. Back 639207: Advantage 2 PCR Kit
638504	SeqAmp™ DNA Polymerase	50 Rxns	USD $147.00
SeqAmp DNA Polymerase is a high-fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Documents Components Image Data Back cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain poly(A)⁺ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back 638504: SeqAmp DNA Polymerase
638509	SeqAmp™ DNA Polymerase	200 Rxns	USD $408.00
SeqAmp DNA Polymerase is a high fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Documents Components Image Data Back 638509: SeqAmp DNA Polymerase

634828

SMARTer® Ultra® Low Input RNA for Illumina® Sequencing - HV

96 Rxns

USD $8601.00

License Statement

ID Number
330	This product is the subject of a technology license agreement for internal research use only. Use of this product other than for research use may require additional licenses. Information on license restrictions or for uses other than research may be obtained by contacting licensing@takarabio.com.

SMARTer Ultra Low Input RNA for Illumina Sequencing - HV allows high-quality cDNA synthesis when starting with very low input amounts of total RNA. The kit is regularly tested with 100 pg of total RNA, and has been shown to perform robust cDNA synthesis with RNA from a single cell (10 pg equivalent). It includes the Advantage 2 PCR Kit for PCR amplification and validation. The kit has been designed and validated to prepare cDNA samples for sequencing and quantitation with the Illumina HiSeq, MiSeq, and Genome Analyzersequencing instruments. SMART technology offers unparalleled sensitivity and unbiased amplification of cDNA transcripts, enabling a direct start from your sample. Most importantly, SMART technology enriches for full-length transcripts and maintains the true representation of the original mRNA transcripts; these factors are critical for transcriptome sequencing and gene expression analysis. This kit accommodates an input volume of up to 9 μl.

Notice to purchaser

Components

Required Products

Cat. #	Product	Size	Price
639207	Advantage® 2 PCR Kit	30 Rxns	USD $181.00
This kit is based on Takara's Advantage 2 Polymerase Mix. It contains all reagents needed for a hot-start PCR, including control template and primer mix. This kit is ideal for PCR applications that require high fidelity, such as cDNA amplification or library construction. Enough reagents are supplied for 30 PCR reactions of 50 μl each. Documents Components Image Data Back Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix *Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq* polymerase mix.** 5 μl of PCR products were run on a 1.1% agarose/EtBr gel. Lane 1: The 0.4-kb TNFR II fragment is readily obtained with Advantage 2. Lane 2: No product is seen with Taq polymerase. Lane M: DNA size marker Back Amplification of various large templates using Advantage 2 Polymerase Mix Amplification of various large templates using Advantage 2 Polymerase Mix. 1–3 μl of each PCR product was run on a 1.1% agarose/EtBr gel. Lane 1: 2.5-kb E. coli DNA polymerase gene amplified from genomic DNA. Lane 2: 3.5-kb bovine pancreatic trypsin inhibitor gene amplified from calf thymus genomic DNA. Lane 3: 5.9-kb human IL-1β gene amplified from human genomic DNA. Lane 4: 8.5-kb human titin cDNA amplified from a SMART Human Skeletal Muscle cDNA library. Lane 5: 18.5-kb λ insert amplified from a λ clone. Lane M: λ/Hind III DNA size marker. Back 639207: Advantage 2 PCR Kit
638504	SeqAmp™ DNA Polymerase	50 Rxns	USD $147.00
SeqAmp DNA Polymerase is a high-fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Documents Components Image Data Back cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain poly(A)⁺ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back 638504: SeqAmp DNA Polymerase
638509	SeqAmp™ DNA Polymerase	200 Rxns	USD $408.00
SeqAmp DNA Polymerase is a high fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Documents Components Image Data Back 638509: SeqAmp DNA Polymerase

634823

SMARTer® Ultra® Low Input RNA for Illumina® Sequencing - HV

24 Rxns

USD $3550.00

License Statement

ID Number
330	This product is the subject of a technology license agreement for internal research use only. Use of this product other than for research use may require additional licenses. Information on license restrictions or for uses other than research may be obtained by contacting licensing@takarabio.com.

Notice to purchaser

Components

Required Products

Cat. #	Product	Size	Price
639207	Advantage® 2 PCR Kit	30 Rxns	USD $181.00
This kit is based on Takara's Advantage 2 Polymerase Mix. It contains all reagents needed for a hot-start PCR, including control template and primer mix. This kit is ideal for PCR applications that require high fidelity, such as cDNA amplification or library construction. Enough reagents are supplied for 30 PCR reactions of 50 μl each. Documents Components Image Data Back Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix *Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq* polymerase mix.** 5 μl of PCR products were run on a 1.1% agarose/EtBr gel. Lane 1: The 0.4-kb TNFR II fragment is readily obtained with Advantage 2. Lane 2: No product is seen with Taq polymerase. Lane M: DNA size marker Back Amplification of various large templates using Advantage 2 Polymerase Mix Amplification of various large templates using Advantage 2 Polymerase Mix. 1–3 μl of each PCR product was run on a 1.1% agarose/EtBr gel. Lane 1: 2.5-kb E. coli DNA polymerase gene amplified from genomic DNA. Lane 2: 3.5-kb bovine pancreatic trypsin inhibitor gene amplified from calf thymus genomic DNA. Lane 3: 5.9-kb human IL-1β gene amplified from human genomic DNA. Lane 4: 8.5-kb human titin cDNA amplified from a SMART Human Skeletal Muscle cDNA library. Lane 5: 18.5-kb λ insert amplified from a λ clone. Lane M: λ/Hind III DNA size marker. Back 639207: Advantage 2 PCR Kit
638504	SeqAmp™ DNA Polymerase	50 Rxns	USD $147.00
SeqAmp DNA Polymerase is a high-fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Documents Components Image Data Back cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain poly(A)⁺ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back 638504: SeqAmp DNA Polymerase
638509	SeqAmp™ DNA Polymerase	200 Rxns	USD $408.00
SeqAmp DNA Polymerase is a high fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Documents Components Image Data Back 638509: SeqAmp DNA Polymerase

634820

SMARTer® Ultra® Low Input RNA for Illumina® Sequencing - HV

12 Rxns

USD $1895.00

License Statement

ID Number
330	This product is the subject of a technology license agreement for internal research use only. Use of this product other than for research use may require additional licenses. Information on license restrictions or for uses other than research may be obtained by contacting licensing@takarabio.com.

Notice to purchaser

Components

Required Products

Cat. #	Product	Size	Price
639207	Advantage® 2 PCR Kit	30 Rxns	USD $181.00
This kit is based on Takara's Advantage 2 Polymerase Mix. It contains all reagents needed for a hot-start PCR, including control template and primer mix. This kit is ideal for PCR applications that require high fidelity, such as cDNA amplification or library construction. Enough reagents are supplied for 30 PCR reactions of 50 μl each. Documents Components Image Data Back Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix *Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq* polymerase mix.** 5 μl of PCR products were run on a 1.1% agarose/EtBr gel. Lane 1: The 0.4-kb TNFR II fragment is readily obtained with Advantage 2. Lane 2: No product is seen with Taq polymerase. Lane M: DNA size marker Back Amplification of various large templates using Advantage 2 Polymerase Mix Amplification of various large templates using Advantage 2 Polymerase Mix. 1–3 μl of each PCR product was run on a 1.1% agarose/EtBr gel. Lane 1: 2.5-kb E. coli DNA polymerase gene amplified from genomic DNA. Lane 2: 3.5-kb bovine pancreatic trypsin inhibitor gene amplified from calf thymus genomic DNA. Lane 3: 5.9-kb human IL-1β gene amplified from human genomic DNA. Lane 4: 8.5-kb human titin cDNA amplified from a SMART Human Skeletal Muscle cDNA library. Lane 5: 18.5-kb λ insert amplified from a λ clone. Lane M: λ/Hind III DNA size marker. Back 639207: Advantage 2 PCR Kit
638504	SeqAmp™ DNA Polymerase	50 Rxns	USD $147.00
SeqAmp DNA Polymerase is a high-fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Documents Components Image Data Back cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain poly(A)⁺ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back 638504: SeqAmp DNA Polymerase
638509	SeqAmp™ DNA Polymerase	200 Rxns	USD $408.00
SeqAmp DNA Polymerase is a high fidelity PCR enzyme with hot start capabilities that is especially well-suited for use with specific SMARTer kits for transcriptome analysis (NGS). This optimized PCR enzyme has been shown to perform well even with challenging templates containing GC-rich and AT-rich regions. DNA Polymerase is sold as part of the SMARTer Stranded RNA-Seq Kit. Documents Components Image Data Back 638509: SeqAmp DNA Polymerase

635007

SMARTer® Stranded Total RNA-Seq Kit - Pico Input Mammalian

96 Rxns

USD $5330.00

License Statement

ID Number
275	SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com.
425	LIMITED USE LABEL LICENSE: RESEARCH USE ONLY Notice to Purchaser: This product is the subject to a license granted to Takara Bio USA, Inc. and its Affiliates from Caribou Biosciences, Inc., and this product is transferred to the end-user purchaser (“Purchaser”) subject to a “Limited Use Label License” conveying to the Purchaser a limited, nontransferable right to use the product, solely as provided to Purchaser, together with (i) progeny or derivatives of the product generated by the Purchaser (including but not limited to cells), and (ii) biological material extracted or derived from the product or its corresponding progeny or derivatives (including but not limited to cells) (collectively, the product, and (i) and (ii) are referred to as “Material”) only to perform internal research for the sole benefit of the Purchaser. The Purchaser cannot sell or otherwise transfer Material to a third party or otherwise use the Material for any Excluded Use. “Excluded Use” means any and all: (a) commercial activity including, but not limited to, any use in manufacturing (including but not limited to cell line development for purposes of bioproduction), product testing, or quality control; (b) preclinical or clinical testing or other activity directed toward the submission of data to the U.S. Food and Drug Administration, or any other regulatory agency in any country or jurisdiction where the active agent in such studies comprises the Material; (c) use to provide a service, information, or data to a third party with the sole exception of using the Material to conduct in vitro sample preparation, i.e., selectively depleting target cDNAs from a sample either by cleaving or selectively separating such target cDNAs from the sample through the use of the Materials; (d) use for human or animal therapeutic, diagnostic, or prophylactic purposes or as a product for therapeutics, diagnostics, or prophylaxis; (e) activity in an agricultural field trial or any activity directed toward the submission of data to the U.S. Department of Agriculture or any other agriculture regulatory agency; (f) high throughput screening drug discovery purposes (i.e., the screening of more than 10,000 experiments per day) as well as scale-up production activities for commercialization; (g) modification of human germline, including editing of human embryo genomes (with the sole exception of editing human embryonic stem (ES) cell lines for research purposes) or reproductive cells; (h) self-editing; and/or (i) stimulation of biased inheritance of a particular gene or trait or set of genes or traits (“gene drive”). It is the Purchaser’s responsibility to use the Material in accordance with all applicable laws and regulations. For information on obtaining additional rights, including commercial rights, please contact licensing@cariboubio.com or Caribou Biosciences, Inc., 2929 7th Street, Suite 105, Berkeley, CA 94710 USA, Attn: Licensing
395	This Product is protected by one or more patents from the family consisting of: US10150985, CA2939621, People's Republic of China Patent: ZL201480077658.0, US10988796, DE602014058059.9, EP3105325, FR3105325, UK3105325, JP6416939 and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing common priority with the same family. Additional information may be found at https://www.takarabio.com/patents.
450	This Product is sold under license from JumpCode Genomics, Inc., and is covered by one or more of the following US patents and foreign counterparts as well as pending US and foreign patent applications: 10,604,802; 11,708,606; 11,761,039; PCT/US2015/014242; CA2938669; EP 20192599.7; HK402021031164.3.

The SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian is used to generate strand-specific RNA-seq libraries for Illumina sequencing using purified total RNA input ranging from 250 pg–10 ng. This kit takes advantage of SMARTer Stranded RNA-seq technology, based on our proprietary SMART (Switching Mechanism at the 5' end of RNA Template) technology, with the added benefits of locked nucleic acid (LNA) technology as in the SMART-Seq v4 kit. The integrated post-cDNA synthesis removal of abundant molecules originating from rRNA makes the workflow extremely sensitive with excellent reproducibility and low mapping to rRNA. This method was developed to work with either high- or low-quality total RNA and does not require additional rRNA removal methods or kits. Final libraries retain strand of origin information.

Notice to purchaser

Components

Required Products

Cat. #	Product	Size	Price	License	Quantity	Details
740815.50	MN Bead Tubes Type E	50 Preps	USD $131.00
MN Bead Tubes are 2-ml screw cap plastic tubes containing different types of beads depending on the application. They are intended for the disruption of biological samples and subsequent nucleic acid purification. MN Bead Tubes Type E consists of a mixture of 40–400 µm glass and 3-mm steel beads for use with hard-to-lyse bacteria within tissue or insect samples using the NucleoSpin Lipid Tissue or NucleoSpin DNA Insect kits. The beads should be used in combination with the MN Bead Tube Holder (Cat. # 740469) and an appropriate vortexer (e.g., Vortex-Genie 2 from Scientific Industries), or with a mixer mill (e.g., from Retsch GmbH). Documents Image Data Back 740815.50: NucleoSpin Bead Tubes Type E

635006

SMARTer® Stranded Total RNA-Seq Kit - Pico Input Mammalian

48 Rxns

USD $4130.00

License Statement

ID Number
275	SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com.
425	LIMITED USE LABEL LICENSE: RESEARCH USE ONLY Notice to Purchaser: This product is the subject to a license granted to Takara Bio USA, Inc. and its Affiliates from Caribou Biosciences, Inc., and this product is transferred to the end-user purchaser (“Purchaser”) subject to a “Limited Use Label License” conveying to the Purchaser a limited, nontransferable right to use the product, solely as provided to Purchaser, together with (i) progeny or derivatives of the product generated by the Purchaser (including but not limited to cells), and (ii) biological material extracted or derived from the product or its corresponding progeny or derivatives (including but not limited to cells) (collectively, the product, and (i) and (ii) are referred to as “Material”) only to perform internal research for the sole benefit of the Purchaser. The Purchaser cannot sell or otherwise transfer Material to a third party or otherwise use the Material for any Excluded Use. “Excluded Use” means any and all: (a) commercial activity including, but not limited to, any use in manufacturing (including but not limited to cell line development for purposes of bioproduction), product testing, or quality control; (b) preclinical or clinical testing or other activity directed toward the submission of data to the U.S. Food and Drug Administration, or any other regulatory agency in any country or jurisdiction where the active agent in such studies comprises the Material; (c) use to provide a service, information, or data to a third party with the sole exception of using the Material to conduct in vitro sample preparation, i.e., selectively depleting target cDNAs from a sample either by cleaving or selectively separating such target cDNAs from the sample through the use of the Materials; (d) use for human or animal therapeutic, diagnostic, or prophylactic purposes or as a product for therapeutics, diagnostics, or prophylaxis; (e) activity in an agricultural field trial or any activity directed toward the submission of data to the U.S. Department of Agriculture or any other agriculture regulatory agency; (f) high throughput screening drug discovery purposes (i.e., the screening of more than 10,000 experiments per day) as well as scale-up production activities for commercialization; (g) modification of human germline, including editing of human embryo genomes (with the sole exception of editing human embryonic stem (ES) cell lines for research purposes) or reproductive cells; (h) self-editing; and/or (i) stimulation of biased inheritance of a particular gene or trait or set of genes or traits (“gene drive”). It is the Purchaser’s responsibility to use the Material in accordance with all applicable laws and regulations. For information on obtaining additional rights, including commercial rights, please contact licensing@cariboubio.com or Caribou Biosciences, Inc., 2929 7th Street, Suite 105, Berkeley, CA 94710 USA, Attn: Licensing
395	This Product is protected by one or more patents from the family consisting of: US10150985, CA2939621, People's Republic of China Patent: ZL201480077658.0, US10988796, DE602014058059.9, EP3105325, FR3105325, UK3105325, JP6416939 and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing common priority with the same family. Additional information may be found at https://www.takarabio.com/patents.
450	This Product is sold under license from JumpCode Genomics, Inc., and is covered by one or more of the following US patents and foreign counterparts as well as pending US and foreign patent applications: 10,604,802; 11,708,606; 11,761,039; PCT/US2015/014242; CA2938669; EP 20192599.7; HK402021031164.3.

Notice to purchaser

Components

635005

SMARTer® Stranded Total RNA-Seq Kit - Pico Input Mammalian

12 Rxns

USD $1442.00

License Statement

ID Number
275	SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by e-mail at licensing@takarabio.com.
425	LIMITED USE LABEL LICENSE: RESEARCH USE ONLY Notice to Purchaser: This product is the subject to a license granted to Takara Bio USA, Inc. and its Affiliates from Caribou Biosciences, Inc., and this product is transferred to the end-user purchaser (“Purchaser”) subject to a “Limited Use Label License” conveying to the Purchaser a limited, nontransferable right to use the product, solely as provided to Purchaser, together with (i) progeny or derivatives of the product generated by the Purchaser (including but not limited to cells), and (ii) biological material extracted or derived from the product or its corresponding progeny or derivatives (including but not limited to cells) (collectively, the product, and (i) and (ii) are referred to as “Material”) only to perform internal research for the sole benefit of the Purchaser. The Purchaser cannot sell or otherwise transfer Material to a third party or otherwise use the Material for any Excluded Use. “Excluded Use” means any and all: (a) commercial activity including, but not limited to, any use in manufacturing (including but not limited to cell line development for purposes of bioproduction), product testing, or quality control; (b) preclinical or clinical testing or other activity directed toward the submission of data to the U.S. Food and Drug Administration, or any other regulatory agency in any country or jurisdiction where the active agent in such studies comprises the Material; (c) use to provide a service, information, or data to a third party with the sole exception of using the Material to conduct in vitro sample preparation, i.e., selectively depleting target cDNAs from a sample either by cleaving or selectively separating such target cDNAs from the sample through the use of the Materials; (d) use for human or animal therapeutic, diagnostic, or prophylactic purposes or as a product for therapeutics, diagnostics, or prophylaxis; (e) activity in an agricultural field trial or any activity directed toward the submission of data to the U.S. Department of Agriculture or any other agriculture regulatory agency; (f) high throughput screening drug discovery purposes (i.e., the screening of more than 10,000 experiments per day) as well as scale-up production activities for commercialization; (g) modification of human germline, including editing of human embryo genomes (with the sole exception of editing human embryonic stem (ES) cell lines for research purposes) or reproductive cells; (h) self-editing; and/or (i) stimulation of biased inheritance of a particular gene or trait or set of genes or traits (“gene drive”). It is the Purchaser’s responsibility to use the Material in accordance with all applicable laws and regulations. For information on obtaining additional rights, including commercial rights, please contact licensing@cariboubio.com or Caribou Biosciences, Inc., 2929 7th Street, Suite 105, Berkeley, CA 94710 USA, Attn: Licensing
395	This Product is protected by one or more patents from the family consisting of: US10150985, CA2939621, People's Republic of China Patent: ZL201480077658.0, US10988796, DE602014058059.9, EP3105325, FR3105325, UK3105325, JP6416939 and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing common priority with the same family. Additional information may be found at https://www.takarabio.com/patents.
450	This Product is sold under license from JumpCode Genomics, Inc., and is covered by one or more of the following US patents and foreign counterparts as well as pending US and foreign patent applications: 10,604,802; 11,708,606; 11,761,039; PCT/US2015/014242; CA2938669; EP 20192599.7; HK402021031164.3.

Notice to purchaser

Components

634833

SMARTer® Ultra® Low RNA Kit for the Fluidigm® C1™ System, 10 IFCs

Each

USD $4233.00

License Statement

ID Number
330	This product is the subject of a technology license agreement for internal research use only. Use of this product other than for research use may require additional licenses. Information on license restrictions or for uses other than research may be obtained by contacting licensing@takarabio.com.

The SMARTer Ultra Low RNA Kit for the Fluidigm C1 System allows high-quality cDNA synthesis starting from 96 single cells that have been isolated and processed with the Fluidigm C1 Single-Cell Auto Prep System. The kit utilizes SMART technology to enrich for full-length transcripts and maintain the true representation of the original mRNA transcripts (critical factors for transcriptome sequencing and gene expression analysis), and includes the Advantage 2 PCR Kit for PCR amplification and validation. The kit has been designed to prepare cDNA samples for transcriptome analysis on any Illumina Genome Analyzer, HiSeq, HiScan or MiSeq sequencing instrument.

Notice to purchaser

Components

634832

SMARTer® Ultra® Low RNA Kit for the Fluidigm® C1™ System, 2 IFCs

Each

USD $1170.00

License Statement

ID Number
330	This product is the subject of a technology license agreement for internal research use only. Use of this product other than for research use may require additional licenses. Information on license restrictions or for uses other than research may be obtained by contacting licensing@takarabio.com.

Notice to purchaser

Components

More Information

Applications

cDNA synthesis or Illumina-compatible library preparation from small quantities of cells or total RNA for transcriptome sequencing
cDNA outputs from these kits can be used with either our SMARTer ThruPLEX DNA-Seq Kit or with the Nextera® XT library prep kits
- cDNA from the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 is also compatible with the Ion Xpress Plus Fragment Library Kit for Ion Torrent sequencing

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2023 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

Legacy RNA-seq kits

Notice to purchaser

Comparison of sequencing metrics generated with either the SMARTer Ultra Low Input RNA for Illumina Sequencing kit (UL-HV) or the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3)

Sequencing results for libraries made from 100 pg Human Brain Total RNA using either the SMARTer Ultra Low Input RNA for Illumina Sequencing kit (UL-HV) or the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3)

Individual cells or 1,000 cells (HeLa or Jurkat; Panel A and Panel B, respectively) were used as input for the SMARTer Ultra Low Input RNA Kit for Sequencing - v3

cDNA libraries were made from 1 or 1,000 cells (HeLa or Jurkat) using the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3)

The gene body coverage of the cDNA libraries made from 1 or 1,000 HeLa cells (blue and red lines, respectively), or 1 or 1,000 Jurkat cells (green and purple lines, respectively) using the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 was determined using RSeQC

Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix

Amplification of various large templates using Advantage 2 Polymerase Mix

639207: Advantage 2 PCR Kit

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

638504: SeqAmp DNA Polymerase

638509: SeqAmp DNA Polymerase

Notice to purchaser

Comparison of sequencing metrics generated with either the SMARTer Ultra Low Input RNA for Illumina Sequencing kit (UL-HV) or the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3)

Sequencing results for libraries made from 100 pg Human Brain Total RNA using either the SMARTer Ultra Low Input RNA for Illumina Sequencing kit (UL-HV) or the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3)

Individual cells or 1,000 cells (HeLa or Jurkat; Panel A and Panel B, respectively) were used as input for the SMARTer Ultra Low Input RNA Kit for Sequencing - v3

cDNA libraries were made from 1 or 1,000 cells (HeLa or Jurkat) using the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3)

The gene body coverage of the cDNA libraries made from 1 or 1,000 HeLa cells (blue and red lines, respectively), or 1 or 1,000 Jurkat cells (green and purple lines, respectively) using the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 was determined using RSeQC

Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix

Amplification of various large templates using Advantage 2 Polymerase Mix

639207: Advantage 2 PCR Kit

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

638504: SeqAmp DNA Polymerase

638509: SeqAmp DNA Polymerase

Notice to purchaser

Comparison of sequencing metrics generated with either the SMARTer Ultra Low Input RNA for Illumina Sequencing kit (UL-HV) or the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3)

Sequencing results for libraries made from 100 pg Human Brain Total RNA using either the SMARTer Ultra Low Input RNA for Illumina Sequencing kit (UL-HV) or the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3)

Individual cells or 1,000 cells (HeLa or Jurkat; Panel A and Panel B, respectively) were used as input for the SMARTer Ultra Low Input RNA Kit for Sequencing - v3

cDNA libraries were made from 1 or 1,000 cells (HeLa or Jurkat) using the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 (UL-v3)

The gene body coverage of the cDNA libraries made from 1 or 1,000 HeLa cells (blue and red lines, respectively), or 1 or 1,000 Jurkat cells (green and purple lines, respectively) using the SMARTer Ultra Low Input RNA Kit for Sequencing - v3 was determined using RSeQC

Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix

Amplification of various large templates using Advantage 2 Polymerase Mix

639207: Advantage 2 PCR Kit

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

638504: SeqAmp DNA Polymerase

638509: SeqAmp DNA Polymerase

Notice to purchaser

Comparison of transcript coverage with different amounts of input RNA

Electropherogram of amplified SMARTer cDNA

Gene expression data obtained from very low amounts of RNA correlate well with data obtained by qPCR

Overview of SMARTer Ultra Low RNA Kit sample preparation for Illumina sequencing

634828: SMARTer Ultra Low Input RNA for Illumina Sequencing - HV

Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix

Amplification of various large templates using Advantage 2 Polymerase Mix

639207: Advantage 2 PCR Kit

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

638504: SeqAmp DNA Polymerase

638509: SeqAmp DNA Polymerase

Notice to purchaser

Comparison of transcript coverage with different amounts of input RNA

Electropherogram of amplified SMARTer cDNA

Gene expression data obtained from very low amounts of RNA correlate well with data obtained by qPCR

Overview of SMARTer Ultra Low RNA Kit sample preparation for Illumina sequencing

Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix

Amplification of various large templates using Advantage 2 Polymerase Mix

639207: Advantage 2 PCR Kit

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

638504: SeqAmp DNA Polymerase

638509: SeqAmp DNA Polymerase

Notice to purchaser

Comparison of transcript coverage with different amounts of input RNA

Electropherogram of amplified SMARTer cDNA

Gene expression data obtained from very low amounts of RNA correlate well with data obtained by qPCR

Overview of SMARTer Ultra Low RNA Kit sample preparation for Illumina sequencing

Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix

Amplification of various large templates using Advantage 2 Polymerase Mix

639207: Advantage 2 PCR Kit

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

638504: SeqAmp DNA Polymerase

638509: SeqAmp DNA Polymerase

Notice to purchaser

High reproducibility across the recommended input range

Schematic of the technologies in the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian

Improved exon mapping and transcript identification from human tissues

Improved exon mapping and transcript identification from rodent tissues

High reproducibility between libraries with or without ribosomal cDNA depletion

Efficient capture of degraded RNA

635007: SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian

740815.50: NucleoSpin Bead Tubes Type E