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Next-generation sequencing

RiboGone - Mammalian—low-input ribosomal RNA removal kit for human, mouse, and rat samples

The RiboGone - Mammalian kit specifically removes ribosomal RNA (rRNA) and mitochondrial RNA (mtRNA) from human, mouse, or rat total RNA samples. RiboGone - Mammalian uses oligo hybridization and RNase H digestion to specifically deplete 5S, 5.8S, 18S, and 28S nuclear rRNA, as well as 12S mtRNA. The kit is designed to work with input RNA of any quality, including degraded material (e.g., FFPE samples). RiboGone - Mammalian is highly suited for sample preparation prior to random-primed cDNA synthesis and RNA-seq or transcriptome analysis applications. When used in tandem with the SMARTer Stranded RNA-Seq Kit or the SMARTer Universal Low Input RNA Library Prep Kit, RiboGone - Mammalian reduces the frequency of rRNA sequences to <5% of total reads.

Cat. #

Product

Size

License

Quantity

Details

634847

RiboGone™ - Mammalian

24 Rxns

USD $1377.00

License Statement

ID Number
246	This product is protected by U.S. Patent 9,428,794, and additional pending US and foreign patents. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

RiboGone - Mammalian allows for the specific removal of rRNA sequences (5S, 5.8S, 18S, and 28S), as well as mitochondrial rRNA sequences (12S), from human, mouse, or rat total RNA. This kit is designed for use with limited sample amounts (10–100 ng of total RNA), and works with high- or low-quality RNA. Samples processed using the RiboGone kit are ready for cDNA synthesis with random primers.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

Required Products

Cat. #	Product	Size	Price
634938	SMARTer® Universal Low Input RNA Kit for Sequencing	10 Rxns	USD $1104.00
The SMARTer Universal Low Input RNA Kit for Sequencing contains the components needed to synthesize high-quality cDNA from as little as 200 pg of RNA, and includes the Advantage 2 PCR Kit for PCR amplification and validation. The kit utilizes both SMART technology and random priming, and has been designed and validated to prepare cDNA samples for sequencing and quantification with next-generation sequencing platforms. While SMART technology offers unparalleled sensitivity and unbiased amplification of cDNA transcripts, random (universal) priming allows for amplification of damaged RNA and maintains the true representation of the original mRNA transcripts. Both of these factors are critical for transcriptome sequencing and gene expression analysis. Documents Components You May Also Like Image Data Back 634938: SMARTer Universal Low Input RNA Kit for Sequencing
634940	SMARTer® Universal Low Input RNA Kit for Sequencing	25 Rxns	USD $2365.00
The SMARTer Universal Low Input RNA Kit for Sequencing contains the components needed to synthesize high-quality cDNA from as little as 200 pg of RNA, and includes the Advantage 2 PCR Kit for PCR amplification and validation. The kit utilizes both SMART technology and random priming, and has been designed and validated to prepare cDNA samples for sequencing and quantification with next-generation sequencing platforms. While SMART technology offers unparalleled sensitivity and unbiased amplification of cDNA transcripts, random (universal) priming allows for amplification of damaged RNA and maintains the true representation of the original mRNA transcripts. Both of these factors are critical for transcriptome sequencing and gene expression analysis. Documents Components You May Also Like Image Data Back 634940: SMARTer Universal Low Input RNA Kit for Sequencing
634836	SMARTer® Stranded RNA-Seq Kit	12 Rxns	USD $1048.00
The SMARTer Stranded RNA-Seq Kit includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of polyA-purified or ribosomal RNA-depleted RNA. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the llumina Indexing Primer Set (PCR primers for the amplification of indexed, paired-end Illumina-compatible sequencing libraries, which enable multiplexing of NGS library analysis). The SMARTer Stranded RNA-Seq Kit utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic clean-up or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA. Documents Components You May Also Like Image Data Resources Back cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain poly(A)⁺ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a 1,000-fold range of input RNA levels The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a 1,000-fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain poly(A)⁺ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log₁₀ scale. Insets indicate the Pearson coefficient of correlation between replicates (R). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log₁₀ scale. Insets indicate the Pearson coefficient of correlation between replicates (R). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back Even at the lowest levels tested [100 pg poly(A)+ input RNA], the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments The SMARTer Stranded RNA-Seq Kit maintains high accuracy even with low input volume. ~15,000 genes were detected using strand-specific alignments, even with only 100 pg poly(A)⁺ input RNA. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R² of 0.9725. Axes are plotted on a log₂ scale. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Takara Bio) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Takara Bio), while retaining more noncoding reads. Back 634836: SMARTer Stranded RNA-Seq Kit
634837	SMARTer® Stranded RNA-Seq Kit	24 Rxns	USD $1887.00
The SMARTer Stranded RNA-Seq Kit includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of polyA-purified or ribosomal RNA-depleted RNA. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the llumina Indexing Primer Set (PCR primers for the amplification of indexed, paired-end Illumina-compatible sequencing libraries, which enable multiplexing of NGS library analysis). The SMARTer Stranded RNA-Seq Kit utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic clean-up or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA. Documents Components You May Also Like Image Data Resources Back cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain poly(A)⁺ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a 1,000-fold range of input RNA levels The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a 1,000-fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain poly(A)⁺ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log₁₀ scale. Insets indicate the Pearson coefficient of correlation between replicates (R). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log₁₀ scale. Insets indicate the Pearson coefficient of correlation between replicates (R). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back Even at the lowest levels tested [100 pg poly(A)+ input RNA], the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments The SMARTer Stranded RNA-Seq Kit maintains high accuracy even with low input volume. ~15,000 genes were detected using strand-specific alignments, even with only 100 pg poly(A)⁺ input RNA. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R² of 0.9725. Axes are plotted on a log₂ scale. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Takara Bio) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Takara Bio), while retaining more noncoding reads. Back 634837: SMARTer Stranded RNA-Seq Kit
634838	SMARTer® Stranded RNA-Seq Kit	48 Rxns	USD $3188.00
The SMARTer Stranded RNA-Seq Kit includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of polyA-purified or ribosomal RNA-depleted RNA. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the llumina Indexing Primer Set (PCR primers for the amplification of indexed, paired-end Illumina-compatible sequencing libraries, which enable multiplexing of NGS library analysis). The SMARTer Stranded RNA-Seq Kit utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic clean-up or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA. Documents Components You May Also Like Image Data Resources Back cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain poly(A)⁺ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a 1,000-fold range of input RNA levels The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a 1,000-fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain poly(A)⁺ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log₁₀ scale. Insets indicate the Pearson coefficient of correlation between replicates (R). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log₁₀ scale. Insets indicate the Pearson coefficient of correlation between replicates (R). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back Even at the lowest levels tested [100 pg poly(A)+ input RNA], the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments The SMARTer Stranded RNA-Seq Kit maintains high accuracy even with low input volume. ~15,000 genes were detected using strand-specific alignments, even with only 100 pg poly(A)⁺ input RNA. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R² of 0.9725. Axes are plotted on a log₂ scale. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Takara Bio) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Takara Bio), while retaining more noncoding reads. Back 634838: SMARTer Stranded RNA-Seq Kit
634839	SMARTer® Stranded RNA-Seq Kit	96 Rxns	USD $4105.00
The SMARTer Stranded RNA-Seq Kit includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of polyA-purified or ribosomal RNA-depleted RNA. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the llumina Indexing Primer Set (PCR primers for the amplification of indexed, paired-end Illumina-compatible sequencing libraries, which enable multiplexing of NGS library analysis). The SMARTer Stranded RNA-Seq Kit utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic clean-up or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA. Documents Components You May Also Like Image Data Resources Back cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain poly(A)⁺ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a 1,000-fold range of input RNA levels The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a 1,000-fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain poly(A)⁺ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log₁₀ scale. Insets indicate the Pearson coefficient of correlation between replicates (R). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log₁₀ scale. Insets indicate the Pearson coefficient of correlation between replicates (R). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back Even at the lowest levels tested [100 pg poly(A)+ input RNA], the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments The SMARTer Stranded RNA-Seq Kit maintains high accuracy even with low input volume. ~15,000 genes were detected using strand-specific alignments, even with only 100 pg poly(A)⁺ input RNA. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R² of 0.9725. Axes are plotted on a log₂ scale. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Takara Bio) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Takara Bio), while retaining more noncoding reads. Back 634839: SMARTer Stranded RNA-Seq Kit

634846

RiboGone™ - Mammalian

6 Rxns

USD $394.00

License Statement

ID Number
246	This product is protected by U.S. Patent 9,428,794, and additional pending US and foreign patents. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.

Notice to purchaser

Components

Required Products

Cat. #	Product	Size	Price
634938	SMARTer® Universal Low Input RNA Kit for Sequencing	10 Rxns	USD $1104.00
The SMARTer Universal Low Input RNA Kit for Sequencing contains the components needed to synthesize high-quality cDNA from as little as 200 pg of RNA, and includes the Advantage 2 PCR Kit for PCR amplification and validation. The kit utilizes both SMART technology and random priming, and has been designed and validated to prepare cDNA samples for sequencing and quantification with next-generation sequencing platforms. While SMART technology offers unparalleled sensitivity and unbiased amplification of cDNA transcripts, random (universal) priming allows for amplification of damaged RNA and maintains the true representation of the original mRNA transcripts. Both of these factors are critical for transcriptome sequencing and gene expression analysis. Documents Components You May Also Like Image Data Back 634938: SMARTer Universal Low Input RNA Kit for Sequencing
634940	SMARTer® Universal Low Input RNA Kit for Sequencing	25 Rxns	USD $2365.00
The SMARTer Universal Low Input RNA Kit for Sequencing contains the components needed to synthesize high-quality cDNA from as little as 200 pg of RNA, and includes the Advantage 2 PCR Kit for PCR amplification and validation. The kit utilizes both SMART technology and random priming, and has been designed and validated to prepare cDNA samples for sequencing and quantification with next-generation sequencing platforms. While SMART technology offers unparalleled sensitivity and unbiased amplification of cDNA transcripts, random (universal) priming allows for amplification of damaged RNA and maintains the true representation of the original mRNA transcripts. Both of these factors are critical for transcriptome sequencing and gene expression analysis. Documents Components You May Also Like Image Data Back 634940: SMARTer Universal Low Input RNA Kit for Sequencing
634836	SMARTer® Stranded RNA-Seq Kit	12 Rxns	USD $1048.00
The SMARTer Stranded RNA-Seq Kit includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of polyA-purified or ribosomal RNA-depleted RNA. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the llumina Indexing Primer Set (PCR primers for the amplification of indexed, paired-end Illumina-compatible sequencing libraries, which enable multiplexing of NGS library analysis). The SMARTer Stranded RNA-Seq Kit utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic clean-up or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA. Documents Components You May Also Like Image Data Resources Back cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain poly(A)⁺ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a 1,000-fold range of input RNA levels The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a 1,000-fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain poly(A)⁺ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log₁₀ scale. Insets indicate the Pearson coefficient of correlation between replicates (R). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log₁₀ scale. Insets indicate the Pearson coefficient of correlation between replicates (R). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back Even at the lowest levels tested [100 pg poly(A)+ input RNA], the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments The SMARTer Stranded RNA-Seq Kit maintains high accuracy even with low input volume. ~15,000 genes were detected using strand-specific alignments, even with only 100 pg poly(A)⁺ input RNA. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R² of 0.9725. Axes are plotted on a log₂ scale. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Takara Bio) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Takara Bio), while retaining more noncoding reads. Back 634836: SMARTer Stranded RNA-Seq Kit
634837	SMARTer® Stranded RNA-Seq Kit	24 Rxns	USD $1887.00
The SMARTer Stranded RNA-Seq Kit includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of polyA-purified or ribosomal RNA-depleted RNA. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the llumina Indexing Primer Set (PCR primers for the amplification of indexed, paired-end Illumina-compatible sequencing libraries, which enable multiplexing of NGS library analysis). The SMARTer Stranded RNA-Seq Kit utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic clean-up or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA. Documents Components You May Also Like Image Data Resources Back cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain poly(A)⁺ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a 1,000-fold range of input RNA levels The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a 1,000-fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain poly(A)⁺ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log₁₀ scale. Insets indicate the Pearson coefficient of correlation between replicates (R). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log₁₀ scale. Insets indicate the Pearson coefficient of correlation between replicates (R). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back Even at the lowest levels tested [100 pg poly(A)+ input RNA], the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments The SMARTer Stranded RNA-Seq Kit maintains high accuracy even with low input volume. ~15,000 genes were detected using strand-specific alignments, even with only 100 pg poly(A)⁺ input RNA. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R² of 0.9725. Axes are plotted on a log₂ scale. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Takara Bio) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Takara Bio), while retaining more noncoding reads. Back 634837: SMARTer Stranded RNA-Seq Kit
634838	SMARTer® Stranded RNA-Seq Kit	48 Rxns	USD $3188.00
The SMARTer Stranded RNA-Seq Kit includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of polyA-purified or ribosomal RNA-depleted RNA. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the llumina Indexing Primer Set (PCR primers for the amplification of indexed, paired-end Illumina-compatible sequencing libraries, which enable multiplexing of NGS library analysis). The SMARTer Stranded RNA-Seq Kit utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic clean-up or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA. Documents Components You May Also Like Image Data Resources Back cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain poly(A)⁺ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a 1,000-fold range of input RNA levels The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a 1,000-fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain poly(A)⁺ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log₁₀ scale. Insets indicate the Pearson coefficient of correlation between replicates (R). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log₁₀ scale. Insets indicate the Pearson coefficient of correlation between replicates (R). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back Even at the lowest levels tested [100 pg poly(A)+ input RNA], the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments The SMARTer Stranded RNA-Seq Kit maintains high accuracy even with low input volume. ~15,000 genes were detected using strand-specific alignments, even with only 100 pg poly(A)⁺ input RNA. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R² of 0.9725. Axes are plotted on a log₂ scale. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Takara Bio) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Takara Bio), while retaining more noncoding reads. Back 634838: SMARTer Stranded RNA-Seq Kit
634839	SMARTer® Stranded RNA-Seq Kit	96 Rxns	USD $4105.00
The SMARTer Stranded RNA-Seq Kit includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of polyA-purified or ribosomal RNA-depleted RNA. The kit consists of the SMARTer Stranded RNA-Seq Components, SeqAmp DNA Polymerase, and the llumina Indexing Primer Set (PCR primers for the amplification of indexed, paired-end Illumina-compatible sequencing libraries, which enable multiplexing of NGS library analysis). The SMARTer Stranded RNA-Seq Kit utilizes our patented SMART (Switching Mechanism At 5' end of RNA Template) technology, coupled with PCR amplification, to generate Illumina-compatible libraries without the need for enzymatic clean-up or adapter ligations. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA. Documents Components You May Also Like Image Data Resources Back cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain poly(A)⁺ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a 1,000-fold range of input RNA levels The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a 1,000-fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain poly(A)⁺ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log₁₀ scale. Insets indicate the Pearson coefficient of correlation between replicates (R). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log₁₀ scale. Insets indicate the Pearson coefficient of correlation between replicates (R). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back Even at the lowest levels tested [100 pg poly(A)+ input RNA], the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments The SMARTer Stranded RNA-Seq Kit maintains high accuracy even with low input volume. ~15,000 genes were detected using strand-specific alignments, even with only 100 pg poly(A)⁺ input RNA. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R² of 0.9725. Axes are plotted on a log₂ scale. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement. Back RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Takara Bio) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Takara Bio), while retaining more noncoding reads. Back 634839: SMARTer Stranded RNA-Seq Kit

Overview

Effective rRNA removal method for human, mouse, and rat total RNA samples—depletes ≥95% of ribosomal RNA sequences
Suitable for any quality RNA, including FFPE samples
Low input working range: 10–100 ng starting material
Works seamlessly with downstream random-primed cDNA synthesis kits like the Stranded RNA-Seq Kit or the SMARTer Universal Low Input RNA Library Prep Kit

Interested in more data and FAQs about this product? Visit the NGS Learning Center.

More Information

Applications

Removal of rRNA from low-input total RNA samples prior to random-primed cDNA synthesis

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2023 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

RiboGone - Mammalian—low-input ribosomal RNA removal kit for human, mouse, and rat samples

Notice to purchaser

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA

Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples

RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA

634847: RiboGone - Mammalian

634938: SMARTer Universal Low Input RNA Kit for Sequencing

634940: SMARTer Universal Low Input RNA Kit for Sequencing

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a 1,000-fold range of input RNA levels

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Even at the lowest levels tested [100 pg poly(A)+ input RNA], the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

634836: SMARTer Stranded RNA-Seq Kit

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a 1,000-fold range of input RNA levels

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Even at the lowest levels tested [100 pg poly(A)+ input RNA], the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

634837: SMARTer Stranded RNA-Seq Kit

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a 1,000-fold range of input RNA levels

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Even at the lowest levels tested [100 pg poly(A)+ input RNA], the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

634838: SMARTer Stranded RNA-Seq Kit

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a 1,000-fold range of input RNA levels

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Even at the lowest levels tested [100 pg poly(A)+ input RNA], the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

634839: SMARTer Stranded RNA-Seq Kit

Notice to purchaser

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA

Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples

RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA

634846: RiboGone - Mammalian

634938: SMARTer Universal Low Input RNA Kit for Sequencing

634940: SMARTer Universal Low Input RNA Kit for Sequencing

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a 1,000-fold range of input RNA levels

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Even at the lowest levels tested [100 pg poly(A)+ input RNA], the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

634836: SMARTer Stranded RNA-Seq Kit

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a 1,000-fold range of input RNA levels

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Even at the lowest levels tested [100 pg poly(A)+ input RNA], the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

634837: SMARTer Stranded RNA-Seq Kit

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a 1,000-fold range of input RNA levels

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Even at the lowest levels tested [100 pg poly(A)+ input RNA], the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

634838: SMARTer Stranded RNA-Seq Kit