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  • ‹ Back to Reporter systems
  • Fluorescent protein promoter reporters
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  • Beta galactosidase and LacZ vectors
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  • In Vivo Luciferase Imaging Kit
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Home › Products › Cell biology assays › Reporter systems › Beta galactosidase and LacZ vectors

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Beta-galactosidase assay and LacZ vectors

The beta-galactosidase assay is commonly used as a reporter, to monitor transfection efficiency, and for promoter/enhancer studies. We offer two ways to detect LacZ gene activity: a colorimetric beta-galactosidase staining kit and a chemiluminescent beta-galactosidase assay.

The beta-galactosidase assay is commonly used as a reporter, to monitor transfection efficiency, and for promoter/enhancer studies. We offer two ways to detect LacZ gene activity: a colorimetric beta-galactosidase staining kit and a chemiluminescent beta-galactosidase assay.

  • Colorimetric assay: The Beta-Galactosidase Staining Kit is a complete system for colorimetric detection of beta-galactosidase expression in mammalian cells. In this kit, beta-galactosidase (the product of the LacZ gene) catalyzes the hydrolysis of X-gal to produce a blue color that is easily visualized. Simply fix, wash, and incubate your cells with the X-Gal Staining Mix. Then use a light microscope to look for the blue stain.
  • Chemiluminescent assay: The Luminescent Beta-Galactosidase Reporter System 3 (which includes vectors; see below) and Detection Kit II (reagents only) provide quantitative measurement of beta-gal activity using a chemiluminescent substrate. The chemiluminescent signal is stable over several hours and can be detected by a scintillation counter, tube and plate luminometers, or by x-ray film exposure.

LacZ vectors

Beta-galactosidase is the product of the LacZ gene. We offer three beta-gal mammalian reporter vectors containing the LacZ gene. The vectors provide maximal flexibility in analyzing the effects of cis-regulatory elements on gene expression. They can also be used to normalize transfection efficiencies.

  • pBeta-Gal-Basic (sold separately as pLacZ-Basic) lacks eukaryotic promoter and enhancer sequences and can serve as a negative control or to clone promoters for characterization.
  • pBeta-Gal-Control (sold separately as pLacZ-Control) contains the SV40 promoter and enhancer sequences and can be used as a positive control or as a reference for comparing the activities of promoters and enhancers.
  • pCMV-LacZ is a constitutive mammalian reporter vector which expresses extremely high levels of beta-galactosidase from the human cytomegalovirus immediate early promoter (PCMV IE) and can be used as a reference or control plasmid. Beta-galactosidase activity can be detected with the Luminescent Beta-galactosidase Detection Kit II.

Complete beta-gal reporter system

The Luminescent Beta-galactosidase Reporter System 3 includes the chemiluminescent beta-galactosidase assay reagents plus the pBeta-Gal-Basic and pBeta-Gal-Control vectors.

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Cat. # Product Size Price License Quantity Details
631780 Beta-Galactosidase Staining Kit 120 Rxns $224.00

The Beta-Galactosidase Staining Kit is a complete system for X-gal detection of beta-galactosidase expression in mammalian cells. The kit contains sufficient reagents to perform 120 beta-galactosidase staining assays in 35 mm dishes.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components
631712 Luminescent Beta-galactosidase Detection Kit II 300 Rxns $487.00

The Luminescent β-galactosidase Detection Kit II is a complete system for the chemiluminescent detection of β-galactosidase enzyme activity. The kit contains all the reagents necessary for 300 chemiluminescent assays to detect enzyme activity. The protocol describes chemiluminescent detection of β-galactosidase via a scintillation counter, tube or plate luminometer, or x-ray film.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Flow chart of a chemiluminescent beta-galactosidase assay

Flow chart of a chemiluminescent beta-galactosidase assay
Flow chart of a chemiluminescent beta-galactosidase assay.

Back

The pBeta gal mammalian reporter vectors

The pBeta gal mammalian reporter vectors
The pBeta gal mammalian reporter vectors.

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The pCMV Beta mammalian reporter vector

The pCMV Beta mammalian reporter vector
The pCMV Beta mammalian reporter vector.
631713 Luminescent Beta-galactosidase Reporter System 3 Each $768.00

The Luminescent β-galactosidase Reporter System 3 is a complete system for the analysis of transcriptional regulation using the reporter enzyme β-galactosidase. The kit contains two pLacZ reporter vectors and reagents necessary for 100 chemiluminescent assays to detect enzyme activity. The protocol describes chemiluminescent detection of β-galactosidase via a scintillation counter, tube or plate luminometer, or x-ray film.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Flow chart of a chemiluminescent beta-galactosidase assay

Flow chart of a chemiluminescent beta-galactosidase assay
Flow chart of a chemiluminescent beta-galactosidase assay.

Back

The pBeta gal mammalian reporter vectors

The pBeta gal mammalian reporter vectors
The pBeta gal mammalian reporter vectors.

Back

The pCMV Beta mammalian reporter vector

The pCMV Beta mammalian reporter vector
The pCMV Beta mammalian reporter vector.
631719 pCMV-LacZ Vector 25 ug $539.00

pCMV-LacZ is a mammalian expression vector designed for the expression of β-galactosidase in mammalian cells from the human cytomegalovirus immediate early gene promoter. The β-gal gene is located within a pair of NotI restriction sites for excision and replacement with another gene of interest. pCMV-LacZ may be used for optimizing transfection conditions, as a control plasmid for other reporter gene constructs, or for expressing genes inserted in place of β-gal.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Flow chart of a chemiluminescent beta-galactosidase assay

Flow chart of a chemiluminescent beta-galactosidase assay
Flow chart of a chemiluminescent beta-galactosidase assay.

Back

The pBeta gal mammalian reporter vectors

The pBeta gal mammalian reporter vectors
The pBeta gal mammalian reporter vectors.

Back

The pCMV Beta mammalian reporter vector

The pCMV Beta mammalian reporter vector
The pCMV Beta mammalian reporter vector.
631707 pLacZ-Basic Vector 20 ug $514.00

pLacZ-Basic uses the E. coli β-galactosidase gene (lacZ) as a reporter gene for analysis of promoter and enhancer sequences.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Flow chart of a chemiluminescent beta-galactosidase assay

Flow chart of a chemiluminescent beta-galactosidase assay
Flow chart of a chemiluminescent beta-galactosidase assay.

Back

The pBeta gal mammalian reporter vectors

The pBeta gal mammalian reporter vectors
The pBeta gal mammalian reporter vectors.

Back

The pCMV Beta mammalian reporter vector

The pCMV Beta mammalian reporter vector
The pCMV Beta mammalian reporter vector.
631709 pLacZ-Control Vector 20 ug $514.00

pLacZ-Control is designed to provide a reference for comparing the activities of promoter and enhancer elements that use the E. coli β-galactosidase gene (lacZ) as a reporter gene. This vector can also be used to normalize transfection efficiencies.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Flow chart of a chemiluminescent beta-galactosidase assay

Flow chart of a chemiluminescent beta-galactosidase assay
Flow chart of a chemiluminescent beta-galactosidase assay.

Back

The pBeta gal mammalian reporter vectors

The pBeta gal mammalian reporter vectors
The pBeta gal mammalian reporter vectors.

Back

The pCMV Beta mammalian reporter vector

The pCMV Beta mammalian reporter vector
The pCMV Beta mammalian reporter vector.

Overview

  • LacZ vectors for transfection and normalization controls
  • Fast, simple colorimetric and chemiluminescent beta-galactosidase assays
  • Complete system includes vectors plus the chemiluminescent beta-galactosidase assay

More Information

Applications

  • Reporter gene assays with the beta-galactosidase reporter
  • Monitoring transfection efficiencies & normalizing transfections
  • Promoter/enhancer studies

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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