Undifferentiated human iPS cells maintained on MEF feeder cells can be readily transferred to the feeder-free DEF-CS culture system. Cryopreserved, MEF-cultured human iPS cells can be thawed directly using the DEF-CS culture system. Fresh cultures should be transferred on days when they would normally be passaged.
- Technical notes
- Getting started with hepatocyte differentiation
- Cardiomyocytes in FLIPR 384-well plate format
- Cardiomyocytes on the Patchliner system
- Cardiomyocytes on the Maestro MEA system
- Cardiomyocytes on the MED64 MEA system
- Cardiomyocytes on the CardioExcyte 96 system
- Cardiomyocytes on the xCELLigence RTCA CardioECR system
- Reprogramming fibroblasts
- Reprogramming PBMCs
- Spin embryoid body formation
- Transferring iPSCs from other media to DEF-CS
- Transferring iPSCs on MEFs to DEF-CS
- Selection guides
Transferring iPSCs on MEFs to the DEF-CS culture system
- Cellartis DEF-CS 500 Culture System (Takara Bio, Cat. # Y30010)
(includes COAT-1, Basal Medium, GF-1, GF-2, and GF-3)
- TrypLE Select Enzyme (1X), no phenol red
(Thermo Fisher Scientific, Cat. # 12563011)
- PBS Dulbecco's with Ca2+ & Mg2+ (D-PBS +/+)
- PBS Dulbecco's w/o Ca2+ & Mg2+ (D-PBS –/–)
- Cell culture vessels, tissue-culture-treated polystyrene surface
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