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Home › Products › Gene function › Tet-inducible expression systems › Tet-On 3G systems › Tet-On 3G systems—bicistronic

Tet-inducible expression systems

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Bicistronic IRES Tet-On 3G inducible expression systems

Use Tet-On 3G for inducible expression of two genes simulataneously

The response vector supplied with these Tet-On 3G tetracycline-inducible expression systems express two transgenes from our premium TRE3G inducible promoter (PTRE3G).

The response vector supplied with these Tet-On 3G tetracycline-inducible expression systems express two transgenes from our premium TRE3G inducible promoter (PTRE3G). You can either co-express two different genes of interest using systems that contain the pTRE3G-IRES Vector, which contains two multiple cloning sites flanking an IRES sequence (Cat. # 631166 and 631346), or you can monitor co-induced expression of your transgene with a fluorescent protein using kits that contain pTRE3G-mCherry (Cat. # 631165 and 631347) or pTRE3G-ZsGreen1 (Cat. # 631164 and 631348).

These IRES-containing bicistronic vectors allow the simultaneous induced expression of two proteins separately but from the same RNA transcript.

How does an IRES work?

The IRES of the encephalomyocarditis virus (ECMV) permits the translation of two open reading frames from one messenger RNA. Although translation initiation of eukaryotic mRNAs occurs almost exclusively at the 5' cap, the IRES allows ribosomes to bind and initiate translation at a second, internal location. Thus, two proteins are expressed simultaneously from the same bicistronic mRNA transcript.

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Cat. # Product Size Price License Quantity Details
631165 Tet-On® 3G Inducible Expression System (with mCherry) Each Inquire for Quotation *

The Tet-On 3G Inducible Expression System (with mCherry) is a powerful, tightly regulated, tetracycline-inducible mammalian expression system that is controlled by the addition of doxycycline to the culture medium. It allows the simultaneous expression of a gene of interest and a red fluorescent protein marker. The system includes all necessary vectors, our highly efficient transfection reagent, Xfect, and Tet System Approved FBS.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Required Products

Cat. # Product Size Price License Quantity Details
631101 Tet System Approved FBS, US-Sourced 500 mL USD $971.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. This FBS has been processed in the United States.

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631106 Tet System Approved FBS 500 mL USD $933.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or its derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems.

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631347 Tet-On® 3G Inducible Expression System (EF1alpha, mCherry) Each Inquire for Quotation *

The Tet-On 3G Inducible Expression System (EF1alpha, mCherry) is a powerful, tightly regulated, tetracycline-inducible mammalian expression system that is controlled by the addition of doxycycline to the culture medium. This system allows the simultaneous expression of a gene of interest and a red fluorescent protein (mCherry) marker under the control of a Tet-responsive promoter that binds the Tet-On 3G transactivator protein in the presence of doxycycline. It includes the pTRE3G-mCherry Vector Set, consisting of an IRES-based vector into which the gene of interest can be cloned and simultaneously expressed with the mCherry marker, a control vector that inducibly expresses luciferase, and two linear selection markers for hygromycin and puromycin resistance—and the pEF1a-Tet3G Vector, which constitutively expresses the Tet-On 3G transactivator protein from a human elongation factor 1 alpha (EF1α) promoter, allowing expression of the protein in a variety of mammalian cells (such as hematopoietic or stem cells) without the transgene silencing associated with CMV promoters. This system also includes our highly efficient Xfect Transfection Reagent and Tet System Approved FBS.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Required Products

Cat. # Product Size Price License Quantity Details
631101 Tet System Approved FBS, US-Sourced 500 mL USD $971.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. This FBS has been processed in the United States.

Documents Components Image Data
631106 Tet System Approved FBS 500 mL USD $933.00

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or its derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems.

Documents Components Image Data

*You must be logged in to a Purchasing Account in order to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@takarabio.com.

Antibodies to GFP variants, mCherry and other fluorescent proteins Fluorescent protein antibodies
Tips and FAQs Learn about Tet systems
Selection guides Tet-On 3G kit options

Overview

  • Tightly controlled inducible expression of two genes of interest from the same bicistronic transcript
  • Monitor induced expression of a single gene using mCherry or ZsGreen1 fluorescent proteins
  • Two transgenes or one transgene and a bright fluorescent protein expressed from a single IRES bicistronic transcript
  • You may alternatively be interested in Tet-On 3G systems that contain a bidirectional promoter

More Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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