Transgene detection primer set
Our Transgene Detection Primer Set for Real Time (Mouse) includes sets of primers designed to amplify sequences corresponding to GFP and lacZ, respectively—two markers commonly inserted into transgenic mice. These primer sets enable screening of transgenic mice by qPCR/real-time PCR.
The Reference primer-1 and Reference primer-2 detect the mouse Ywhaz and Raver2 genes, respectively, and are included with the kit as references. These reference primers enable comparisons of relative transgene quantities between samples.
- Detection and quantification of mouse genome transgenes
(for 100 reactions1 in each primer)
|GFP primer-12||2 µM each||500 µl||127 bp|
|GFP primer-23||2 µM each||500 µl||162 bp|
|lacZ primer-14||2 µM each||500 µl||96 bp|
|lacZ primer-24||2 µM each||500 µl||141 bp|
|Reference primer-15||2 µM each||500 µl||115 bp|
|Reference primer-26||2 µM each||500 µl||89 bp|
1 When using SYBR Premix Ex Taq II with 25 µl reaction volume.
2 GFP primer-1 is designed for detection of EGFP and AcGFP1 sequences.
3 GFP primer-2 is designed for detection of EGFP sequence. It can not be used for AcGFP1 detection.
4 acZ primer-1 and -2 are designed for lacZ (beta-galactosidase) gene detection and target different gene sequence sites.
5 Reference primer-1 is for a part of the Ywhaz gene on mouse chromosome 15.
6 Reference primer-2 is for a part of the Raver2 gene on mouse chromosome 4.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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