Transgene detection primer set
Our Transgene Detection Primer Set for Real Time (Mouse) includes sets of primers designed to amplify sequences corresponding to GFP and lacZ, respectively—two markers commonly inserted into transgenic mice. These primer sets enable screening of transgenic mice by qPCR/real-time PCR.
Reference primer-1 and Reference primer-2 detect the mouse Ywhaz and Raver2 genes, respectively, and are included with the kit as references. They enable comparisons of relative transgene quantities between samples.
- Detection and quantification of mouse genome transgenes
Sufficient for 100 reactions with each primer when using TB Green Premix Ex Taq II with 25 µl reaction volumes.
|GFP primer-11||2 µM each||500 µl||127 bp|
|GFP primer-22||2 µM each||500 µl||162 bp|
|lacZ primer-13||2 µM each||500 µl||96 bp|
|lacZ primer-23||2 µM each||500 µl||141 bp|
|Reference primer-14||2 µM each||500 µl||115 bp|
|Reference primer-25||2 µM each||500 µl||89 bp|
1GFP primer-1 is designed for detection of EGFP and AcGFP1 sequences.
2GFP primer-2 is designed for detection of EGFP sequence. It cannot be used for AcGFP1 detection.
3lacZ primer-1 and -2 are designed for lacZ (beta-galactosidase) gene detection and target different gene sequences.
4Reference primer-1 is designed for a part of the Ywhaz gene on mouse chromosome 15.
5Reference primer-2 is designed for a part of the Raver2 gene on mouse chromosome 4.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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